Electrochemical-Based Biosensors for the Detection and Diagnosis of Common Pathogens

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Traditional methods currently used for the detection of microorganisms are insufficient in situations requiring rapid intervention due to need laboratory equipment and, particularly, long time required results. According World Health Organization (WHO), biosensors characterized as affordable, sensitive, specific, user-friendly, fast, durable, and equipment-free systems represent a major alternative existing methods. For this reason, one most recent technologies have emerged over past few decades. Among types biosensors, electrochemical stand out they simple use, allow with minimal sample amounts, adaptable portable measuring devices, making them suitable field use. Systems developed detect crucial, can identify foodborne pathogens hospital infections, both which require detection. In review, we present sensors sensitive limits significant bacteria causing comparing different

Language: Английский

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Next-Generation Optical Biosensors: Cutting-Edge Advances in Optical Detection Methods

Plasmonics, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Language: Английский

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Nitrogen-Doped Graphene Quantum Dots (N-GQDs): A Promising Material for the Development of Electrochemical Immunosensors

ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

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Ultrasensitive Peptide-Based Electrochemical Biosensor for Universal Diagnostic of Dengue

Biosensors, Journal Year: 2025, Volume and Issue: 15(4), P. 236 - 236

Published: April 8, 2025

Dengue is a neglected disease mainly affecting tropical and subtropical countries. The diagnosis of dengue fever still problem since most it made from whole or recombinant DENV proteins, which present cross-reactions with other members the Flavivirus family. Therefore, there huge demand for new diagnostic methods that provide rapid, low-cost, easy-to-use confirmation. Thus, in this study, we developed an affordable electrochemical biosensor rapidly detecting immunoglobulin G (IgG) serological antibodies sera DENV-infected patients. An identified linear B-cell epitope (DENV/18) specific 1–4 serotypes recognized by IgG patient was selected as target molecule after microarray peptides using SPOT-synthesis methodology. After chemical synthesis, DENV/18-peptide immobilized on surface working electrode commercially available screen-printed gold (SPGE). capture DENV-specific allowed formation immunocomplex measured cyclic voltammetry (CV) differential pulse (DPV) potassium ferrocyanide/ferricyanide ([Fe(CN)6]3−/4−) probe. evaluation biosensor’s performance showed detection limit 100 µg mL−1 synthetic 1.21 ng CV 0.43 DPV human serum, sensitivity 7.21 µA 8.79 DPV. differentiation infected uninfected individuals possible even at high dilution factor reduced required sample volumes to few microliters. final device proved suitable diagnosing analyzing real serum samples, results good agreement molecular biology diagnostics. flexibility conjugate antigenic SPEs suggests technology could be adapted diagnose pathogens.

Language: Английский

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DFT insights into assembling [8]MCPP with [14]pyridine nanobelts for amino acid sensing

Journal of Molecular Graphics and Modelling, Journal Year: 2025, Volume and Issue: unknown, P. 109056 - 109056

Published: April 1, 2025

Language: Английский

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A perspective on the selection and design of antimicrobial peptides for bacteria detection

Sensing and Bio-Sensing Research, Journal Year: 2025, Volume and Issue: unknown, P. 100794 - 100794

Published: April 1, 2025

Language: Английский

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Nanosensor based on HP‐MAP1 and carbon nanotubes for bacteria detection

Biotechnology Progress, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

Abstract Healthcare‐associated infections (HAIs) pose significant challenges to global health due pathogen complexity and antimicrobial resistance. Biosensors utilizing peptides offer innovative solutions. Hylarana picturata Multiple Active Peptide 1 (Hp‐MAP1), derived from Temporin‐PTA, exhibits antibacterial properties sourced the skin secretions of Malaysian fire‐bellied frog. An sensing layer was developed for electrochemical biorecognition diverse pathogens: Pseudomonas aeruginosa , Klebsiella pneumoniae Escherichia coli Staphylococcus aureus . Electrochemical impedance spectroscopy differentiated microorganisms based on distinct responses. The sensor layer, composed functionalized multi‐walled carbon nanotubes (MWCNTs) associated with Hp‐MAP1, exhibited varying levels charge transfer resistance ( R CT ) different microorganisms. Gram‐negative species, especially P. displayed higher values, indicating better impedimetric Excellent LODs were observed (0.60), K. (0.42), E. (0.67), S. (0.59), highlighting efficacy MWCNTs/Hp‐MAP1 biosensor in microbial identification. platform presents a promising effective identification strategy potential healthcare applications mitigate HAIs enhance patient care.

Language: Английский

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Norovirus Detection Technologies: From Conventional Methods to Innovative Biosensors

Analytical Biochemistry, Journal Year: 2024, Volume and Issue: unknown, P. 115750 - 115750

Published: Dec. 1, 2024

Language: Английский

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