Foodborne bacterial pathogens: genome-based approaches for enduring and emerging threats in a complex and changing world

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(9), P. 543 - 555

Published: May 24, 2024

Language: Английский

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High-throughput analysis of microbiomes in a meat processing facility: are food processing facilities an establishment niche for persisting bacterial communities?

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 27, 2025

Microbial spoilage in meat impedes the development of sustainable food systems. However, our understanding origin microbes is limited. Here, we describe a detailed longitudinal study that assesses microbial dynamics processing facility using high-throughput culture-dependent and culture-independent approaches to reveal diversity, dispersal, persistence, biofilm formation spoilage-associated microbes. Culture-dependent revealed large diversity within facility, including 74 undescribed bacterial taxa multiple Ten out 10 reconstituted communities formed biofilms, biomass was generally higher at 4 °C than 25 °C. Isolates obtained different sampling times or from sites differed fewer genome-wide single-nucleotide polymorphisms were considered same (persistent) strains. Strains Carnobacterium maltaromaticum Rahnella rivi persisted over period 6 months across time, stemming floor drains cooler room. Meat isolates divergens, inusitata, Serratia proteamaculans originated contact non-food environments packaging area. isolation, complemented by analyses, essential fully uncover facilities. populations permanently resided serving as source transmission The ability these coexist form biofilms facilitates their persistence. Our data together with prior on persistence Listeria monocytogenes indicates facilities rule rather an exception.

Language: Английский

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Biofilm formation in food industries: Challenges and control strategies for food safety

Food Research International, Journal Year: 2024, Volume and Issue: 190, P. 114650 - 114650

Published: June 15, 2024

Language: Английский

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Repeated biocide treatments cause changes to the microbiome of a food industry floor drain biofilm model

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 14, 2025

There is a concern about the development of microbial tolerance and resistance to biocides due their repeated use within food industry. This study aimed develop floor drain biofilm model test whether biocide treatment would result in increased biocides. Culturomics shotgun metagenomic analysis 14 drains 214 bacterial isolates from three industrial production environments revealed microbiomes with great diversity complexity, but dominance few highly abundant taxa, including Pseudomonas. A representative was created (3 days, 15°C) using 31 whole genome sequenced 24 genera. The represented 47-58% 76-81% abundance observed metagenome viable microbiota, respectively. exposed on days 3 6 water or different concentrations benzalkonium chloride (BC), peracetic acid (PAA), sodium hypochlorite (SH). Analysis survivors MALDI-TOF MS regrowing biofilms 16S rRNA amplicon sequencing showed how decreased without any change as seen log reductions (CFU/cm2). did, however, exert significantly selective pressures Citrobacter, Acinetobacter, Aeromonas, Pseudomonas dominated after treatments SH PAA, while Serratia Moraxella BC. marcescens could be explained by carriage BC efflux pump (oqxB) highest (20 mg/L BC) minimum inhibitory concentration (MIC) isolates. In contrast, despite carrying (qacH), Listeria monocytogenes ST121 did not show survival presence treatments. Only tested PAA able completely eradicate L. monocytogenes. developed enabled better understanding affect microbiome. Future research should involve testing rotation strategies control regrowth inactivation persistent foodborne pathogens drains.

Language: Английский

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Multispecies Biofilm Cities and the Importance of the Order of Colonization

Food Control, Journal Year: 2025, Volume and Issue: unknown, P. 111319 - 111319

Published: March 1, 2025

Language: Английский

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Listeria monocytogenes and Listeriosis: The Global Enigma

Foods, Journal Year: 2025, Volume and Issue: 14(7), P. 1266 - 1266

Published: April 3, 2025

Listeria monocytogenes is an intracellular, Gram-positive, non-spore-forming, non-encapsulated, facultative anaerobic, rod-shaped, and psychrotrophic food-borne pathogen that causes the infection, listeriosis, thus it attracts great attention following listeriosis outbreaks, which are often associated with high mortality rates. The prevalence of quite low globally; however, most recent deadliest outbreak occurred in South Africa, during 216 persons lost their lives. L. endowed potential to multiply through a wide range harsh environmental conditions, forming biofilms on varying surfaces food industry, as well having persistent antibiotic-resistant cells, pose major threat burden ready-to-eat industry. A more frustrating characteristic this bacterium its strain divergence, alongside increased level antibiotic resistance registered among strains recovered from food, humans, sources, especially those antibiotics involved treatment human listeriosis. Antibiotic exerted by pathogenic microbes ongoing public health menace continues be issue. Against background, thorough search into different databases using various engines was performed, led gathering salient information organised, chronologically, based Altogether, findings elaborated study present up-to date knowledge aspects will improve our understanding mystery ways prevent control dissemination foods. In addition, constant monitoring profiles sources detected changes, giving update trend resistance. Overall, bacterial contamination serves key aspect safety output

Language: Английский

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A formal analysis of Listeria monocytogenes cross-contamination dynamics in multi-species biofilms: the role of coexisting microbiota

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

The risk of listeriosis is primarily linked to ready-to-eat (RTE) foods contaminated, raising concern among food producers. Contamination usually occurs in processing environments (FPE), where L. monocytogenes can coexist with other bacterial species polymicrobial biofilms. However, the transfer bacterium has focused almost exclusively on single-species biofilms, and role coexisting microbiota largely unknown. Therefore, this study addressed dynamics from multi-species biofilms cold-smoked salmon following a stochastic approach. Three (F96, F107, F168) were formed by different consortia composed several strains which it coexists plants, under conditions that reproduced low levels commonly found them, thus mimicking real contamination events. Transfer was performed 25 successive contacts each biofilm fillets, high number replicates (20) used order record stochasticity process. rates (TR) determined, fitted well-known distributions (gamma, Weibull log-normal), then modelled using Monte Carlo method. Similar defined for all three Next, TRs non-significantly values grouped into clusters, probability density functions cluster. This approach showed profiles same applied (L96) monocytogenes. Thus, observed (F96) resulted higher than (L96), revealing major microbiota. Additionally, survival growth capacity refrigerated smoked when transferred implying significantly safety. Being overwhelmingly prevalent FPEs, research provides basis scenarios should be incorporated challenge studies, ultimately leading more effective control manufacturers.

Language: Английский

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From discovery to potential application: engineering a novel M23 peptidase to combat Listeria monocytogenes

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 5, 2025

Language: Английский

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Exploring the occurrence of Listeria in biofilms and deciphering the bacterial community in a frozen vegetable producing environment

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 10, 2024

The establishment of Listeria (L.) monocytogenes within food processing environments constitutes a significant public health concern. This versatile bacterium demonstrates an exceptional capacity to endure challenging environmental conditions in the environment, where contamination products regularly occurs. diverse repertoire stress resistance genes, potential colonize biofilms, and support co-existing microbiota have been proposed as root causes for survival L. environments. In this study, 71 sites were sampled after cleaning disinfection European frozen vegetable facility, in-house clones persisted years. innocua detected by culture-dependent method at 14 sampling sites, primarily on conveyor belts associated parts. presence determined quantification bacterial load analysis extracellular matrix components (carbohydrates, proteins, DNA) was confirmed nine (12.7%). two cases, biofilm. Furthermore, we explored resident microbial community environment biofilm-positive well co-occurrence taxa with 16S rRNA gene sequencing. Pseudomonas , Acinetobacter Exiguobacterium dominated environment. Using differential abundance analysis, amplicon sequence variants (ASVs) assigned Enterobacterales ( Enterobacter Serratia unclassified Enterobacteriaceae ) Carnobacterium found be significantly higher abundant -positive samples. Several ASVs less compared -negative Acinetobacter, Janthinobacterium Brevundimonas key players more relatively biofilms. Further, composition varied between different areas surface materials.

Language: Английский

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Microbial composition and dynamics in environmental samples from a ready-to-eat food production facility with a long-term colonisation of Listeria monocytogenes

Food Microbiology, Journal Year: 2024, Volume and Issue: 125, P. 104649 - 104649

Published: Oct. 5, 2024

Language: Английский

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