The battle against dental caries: defeating biofilm formed by bacterial isolates using vanillin and plant essential oils: in vitro and ex vivo approaches DOI Creative Commons

Manar M. Ahmed,

Nehal Yousef,

Momen Askoura

et al.

Future Journal of Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 10(1)

Published: Oct. 25, 2024

Abstract Background Infections caused by biofilm-forming bacteria have significantly linked to dental plaque and caries. The aim of this study is assess efficacy some natural compounds in inhibition eradication biofilm formed bacterial isolates from infections. Results Bacterial were recovered plaque/caries identified using standard microbiological tests 16S rDNA sequencing. isolated strains include Staphylococcus aureus , epidermidis Enterococcus faecalis Klebsiella pneumoniae Escherichia coli . antibiotic susceptibility was determined disk diffusion method revealed that the majority showed high resistance, 61% found be multidrug resistant. formation capacity investigated microtiter plate assay. Among 77 isolates, seventeen moderate capacity, twenty-two near-moderate, thirty-four had weak four non-biofilm producers. antibiofilm activity tested (rose jasmine oils, propolis, vanillin, vinegar) evaluated against with highest capacity. vitro ability substances alone or combination each other evaluate their prevent destroy preformed single-/multispecies biofilms. Finally, ex vivo on teeth. Our results vanillin rose oils promising activities both models. Conclusions Dental caries can successfully prevented these incorporated new anticaries formulations.

Language: Английский

Hybrid Molecules of Benzothiazole and Hydroxamic Acid as Dual-Acting Biofilm Inhibitors with Antibacterial Synergistic Effect against Pseudomonas aeruginosa Infections DOI

Z. Zhang,

Siyu Zhao,

Wen-Qian Liu

et al.

Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

The ubiquitous opportunistic pathogen Pseudomonas aeruginosa (P. aeruginosa) causes biofilm-associated drug-resistant infections that often lead to treatment failure. Targeting the bacterium's quorum sensing (QS) and iron homeostasis presents a promising strategy combat biofilm formation. This study synthesized benzothiazole-conjugated hydroxamic acid derivatives as dual-acting inhibitors, compound JH21 was identified hit with potent submicromolar inhibitory activity (IC50 = 0.4 μM). Further mechanistic studies demonstrated not only production of virulence decreased through mainly inhibiting QS system but also competed for high-affinity siderophore pyoverdine, inducing deficiency biofilm. Moreover, significantly enhanced efficacy tobramycin ciprofloxacin by 200- 1000-fold, respectively, in mouse wound infection model. These results emphasized feasibility inhibitors against resistant P. potential novel antibacterial synergist.

Language: Английский

Citations

0
A guide to publishing in Biofilm: how to avoid a desk rejection DOI Creative Commons
Tom Coenye, Darla M. Goeres, Birthe V. Kjellerup

et al.

Biofilm, Journal Year: 2025, Volume and Issue: unknown, P. 100282 - 100282

Published: April 1, 2025

Language: Английский

Citations

0
Integrated transcriptomics and metabolomics study on the biofilm formation of Haemophilus influenzae by the stimulation of amoxicillin-clavulanate at subinhibitory concentration DOI
Jiying Xiao, Lin Su, Shumin Huang

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: 205, P. 107650 - 107650

Published: April 29, 2025

Language: Английский

Citations

0
Conformational change as a mechanism for toxin activation in bacterial toxin-antitoxin systems DOI
Viviana Sanchez‐Torres,

Hyeon‐Ji Hwang,

Thomas K. Wood

et al.

Journal of Virology, Journal Year: 2024, Volume and Issue: 98(11)

Published: Oct. 24, 2024

ABSTRACT Toxin/antitoxin (TA) systems are present in nearly every prokaryotic genome and play the important physiological roles of phage inhibition by reducing metabolism (this includes persistence for extreme case complete cessation metabolism), genetic element stabilization, biofilm formation. TA have also been incorporated into other cell systems, such as CRISPR-Cas quorum sensing. For simplest best-studied case, proteinaceous toxins antitoxins (i.e., type II), toxin activity is masked direct binding antitoxin. A long-standing, unresolved question field how activated when bound to at nanomolar affinity. The current paradigm envisions preferential degradation antitoxin a protease, but this highly unlikely that protease cannot discriminate between because both structured. Strikingly, recent results from several studies show one likely mechanism activation conformational changes complex result release or protein trigger, phages, thermally-driven refolding dynamics.

Language: Английский

Citations

1
The battle against dental caries: defeating biofilm formed by bacterial isolates using vanillin and plant essential oils: in vitro and ex vivo approaches DOI Creative Commons

Manar M. Ahmed,

Nehal Yousef,

Momen Askoura

et al.

Future Journal of Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 10(1)

Published: Oct. 25, 2024

Abstract Background Infections caused by biofilm-forming bacteria have significantly linked to dental plaque and caries. The aim of this study is assess efficacy some natural compounds in inhibition eradication biofilm formed bacterial isolates from infections. Results Bacterial were recovered plaque/caries identified using standard microbiological tests 16S rDNA sequencing. isolated strains include Staphylococcus aureus , epidermidis Enterococcus faecalis Klebsiella pneumoniae Escherichia coli . antibiotic susceptibility was determined disk diffusion method revealed that the majority showed high resistance, 61% found be multidrug resistant. formation capacity investigated microtiter plate assay. Among 77 isolates, seventeen moderate capacity, twenty-two near-moderate, thirty-four had weak four non-biofilm producers. antibiofilm activity tested (rose jasmine oils, propolis, vanillin, vinegar) evaluated against with highest capacity. vitro ability substances alone or combination each other evaluate their prevent destroy preformed single-/multispecies biofilms. Finally, ex vivo on teeth. Our results vanillin rose oils promising activities both models. Conclusions Dental caries can successfully prevented these incorporated new anticaries formulations.

Language: Английский

Citations

0