A Hydrogen Sulfide‐Activated NIR‐II Fluorescence/NIR‐I Photoacoustic Dual‐Ratiometric Nanoprobe With Unique Recognition Reaction for Precise Visual Diagnosis of Hepatitis Disease DOI
Lixian Huang, Fei Lv,

Yidong Bin

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Hydrogen sulfide (H 2 S) is a vital gaseous signaling molecule that plays central role in various physiological and pathological processes. Given the complementary advantages of fluorescence (FL) photoacoustic (PA) imaging, there growing demand for dual‐ratiometric probes enable precise vivo monitoring H S levels. In this study, use 2‐mercapto‐1,3,4‐thiadiazole (MTD) as novel recognition group presented first time, following conjugation with cyanine dyes to obtain new PA probe Cy‐MTD. To achieve Cy‐MTD incorporated into down‐conversion nanoparticle (DCNP), resulting creation pioneering NIR‐II FL/NIR‐I nanoprobe DCNP@Cy‐MTD. undergoes blueshift absorption from 840 670 nm after reaction S, enabling NIR‐I ratiometric imaging by measuring ratio signal at (PA /PA ). addition, due strong ≈840 overlapping between spectrum 808 excitation band DCNP, nm‐excited FL emission (F 1550 nm,808Ex ) DCNP DCNP@Cy‐MTD quenched through competitive absorption, while it restored upon interaction because Using stable under 980 nm,980Ex reference signal, /F achieved. The response features offer significant advancement over traditional single‐signal or single‐modality techniques. By providing enhanced accuracy reliability, allows diagnosis hepatitis surpassing capabilities conventional histopathological methods, which provides way more effective diagnostic strategies liver diseases.

Language: Английский

Frontiers in Fluorescence imaging: Tools for the In-Situ Sensing of Disease Biomarkers DOI
Lei Yang, Hongwei Hou, Jinghong Li

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

A comprehensive overview of recent advancements in fluorescence imaging techniques for situ sensing various biomarkers, emphasizing the transformative potential artificial intelligence shaping future bioimaging.

Language: Английский

Citations

4
Zero-Crosstalk Tumor-Targeting Ratiometric Near-Infrared γ-Glutamyltranspeptidase Probe for Fluorescent-Guided Surgical Resection of Orthotopic Hepatic Tumor DOI
Zhichao Yang,

ZhiHao Yang,

Zhiqiang Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

The challenge of "false positive" signals significantly complicates tumor localization and surgical resection, which are pivotal for successful surgeries. Therefore, the development a method preoperative intraoperative margin determination holds considerable promise improving outcomes. In this study, zero-crosstalk ratiometric tumor-targeting near-infrared (NIR) fluorescent probe was developed precise cancer diagnosis navigation via NIR fluorescence imaging. This integrates moiety that selectively homes in on hepatocellular carcinoma cells exhibits highly sensitive response to γ-glutamyltranspeptidase (GGT), characterized by substantial emission shift from 830 650 nm. unique property enhances its effectiveness early imaging-guided surgery resection. Furthermore, successfully monitors GGT activity blood, cells, vivo, endowing potential clinic. Due efficient targeting situ both subcutaneous tumors orthotopic hepatic tumors, accurate detection capability tumors. Additionally, leveraging imaging, can serve as sprayable tool delineating margins with precision, thereby furnishing real-time imaging guidance during resection surgery.

Language: Английский

Citations

0
A novel near-infrared fluorescent probe with large Stokes shift for monitoring γ-glutamyltranspeptidase in tumor and liver injury mice DOI
Zhiqing Wang, Juan Liu,

Zhi-Ke Tan

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: 209, P. 112668 - 112668

Published: Jan. 4, 2025

Language: Английский

Citations

0
A Dual‐Response DNA Origami Platform for Imaging and Treatment of Sepsis‐Associated Acute Kidney Injury DOI Creative Commons
Yingying Zhao,

Yadan Zhao,

Yufan Ling

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Abstract Current diagnostics for sepsis‐associated acute kidney injury (SA‐AKI) detect damage only at advanced stages, limiting opportunities timely intervention. A DNA origami‐based nanoplatform is developed the early diagnosis and treatment of SA‐AKI. Modified with a fluorophore (Cy5) quencher (BHQ3), origami remains nonfluorescent under normal conditions. During SA‐AKI, elevated microRNA‐21 triggers strand displacement reaction that restores fluorescence signal, enabling real‐time detection. Additionally, photoacoustic changes BHQ3, driven by different excretion rates nanostructure released strands, enable dual‐mode imaging, enhancing diagnostic accuracy. Therapeutically, scavenges reactive oxygen species and, when conjugated antimicrobial peptide Leucine‐Leucine‐37 (LL‐37), exhibits bactericidal effects. This combination boosts survival 80% in SA‐AKI models. dual‐response integrates precise imaging targeted therapy, offering powerful strategy management advancing applications precision nanomedicine.

Language: Английский

Citations

0
Dual-miRNA Guided in-vivo Imaging and Multimodal Nanomedicine Approaches for Precise Hepatocellular Carcinoma Differentiation and Synergistic Cancer Theranostics using DNA Hairpins and Dual-Ligand functionalized Zirconium-MOF Nanohybrids DOI
Kun Wang, Siyu Jiang, Wanli Wang

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: unknown, P. 123330 - 123330

Published: April 1, 2025

Language: Английский

Citations

0
Fluorescent probes in autoimmune disease research: current status and future prospects DOI Creative Commons
Junli Chen, Mingkai Chen, Xiaolong Yu

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 9, 2025

Autoimmune diseases (AD) present substantial challenges for early diagnosis and precise treatment due to their intricate pathogenesis varied clinical manifestations. While existing diagnostic methods strategies have advanced, sensitivity, specificity, real-time applicability in settings continue exhibit significant limitations. In recent years, fluorescent probes emerged as highly sensitive specific biological imaging tools, demonstrating potential AD research.This review examines the response mechanisms historical evolution of various types probes, systematically summarizing latest research advancements application autoimmune diseases. It highlights key applications biomarker detection, dynamic monitoring immune cell functions, assessment drug efficacy. Furthermore, this article analyzes technical currently encountered probe development proposes directions future research. With ongoing materials science, nanotechnology, bioengineering, are anticipated achieve higher sensitivity enhanced functional integration, thereby facilitating monitoring, innovative Overall, possess scientific significance value both related diseases, signaling a new era personalized precision medicine.

Language: Английский

Citations

0
A Hydrogen Sulfide‐Activated NIR‐II Fluorescence/NIR‐I Photoacoustic Dual‐Ratiometric Nanoprobe With Unique Recognition Reaction for Precise Visual Diagnosis of Hepatitis Disease DOI
Lixian Huang, Fei Lv,

Yidong Bin

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Hydrogen sulfide (H 2 S) is a vital gaseous signaling molecule that plays central role in various physiological and pathological processes. Given the complementary advantages of fluorescence (FL) photoacoustic (PA) imaging, there growing demand for dual‐ratiometric probes enable precise vivo monitoring H S levels. In this study, use 2‐mercapto‐1,3,4‐thiadiazole (MTD) as novel recognition group presented first time, following conjugation with cyanine dyes to obtain new PA probe Cy‐MTD. To achieve Cy‐MTD incorporated into down‐conversion nanoparticle (DCNP), resulting creation pioneering NIR‐II FL/NIR‐I nanoprobe DCNP@Cy‐MTD. undergoes blueshift absorption from 840 670 nm after reaction S, enabling NIR‐I ratiometric imaging by measuring ratio signal at (PA /PA ). addition, due strong ≈840 overlapping between spectrum 808 excitation band DCNP, nm‐excited FL emission (F 1550 nm,808Ex ) DCNP DCNP@Cy‐MTD quenched through competitive absorption, while it restored upon interaction because Using stable under 980 nm,980Ex reference signal, /F achieved. The response features offer significant advancement over traditional single‐signal or single‐modality techniques. By providing enhanced accuracy reliability, allows diagnosis hepatitis surpassing capabilities conventional histopathological methods, which provides way more effective diagnostic strategies liver diseases.

Language: Английский

Citations

0