3D bioprinting of the airways and lungs for applications in tissue engineering and in vitro models DOI Creative Commons
Yanning Zhang, Yujian Liu, Chen Shu

et al.

Journal of Tissue Engineering, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 1, 2024

Tissue engineering and in vitro modeling of the airways lungs respiratory system are substantial research clinical importance. In airway lung models aim to improve treatment options for repair advance pathophysiological research. The construction biomimetic native with tissue-specific biological, mechanical, configurable features remains challenging. Bioprinting, an emerging 3D printing technology, is promising development airway, lung, disease models, allowing incorporation cells biologically active molecules into printed constructs a precise reproducible manner recreate airways, architecture, microenvironment. Herein, we present review bioprinting applications tissue modeling. key characteristics interstitium, alveoli described. bioinks recently used summarized. Furthermore, propose bioink categorization based on structural airways. Finally, challenges opportunities biofabrication discussed.

Language: Английский

Evaluating inkjet printability of viscoelastic ink through Deborah number analysis DOI

Jules M. Richardot,

Seongju Kim, Sungjune Jung

et al.

Physics of Fluids, Journal Year: 2025, Volume and Issue: 37(2)

Published: Feb. 1, 2025

Digital inkjet printing has been developed as a pivotal technology for precise, scalable, and cost-effective deposition of functional inks, enabling significant advancements in flexible printed electronics bioprinting applications. Analysis Deborah number (De), defined the ratio between relaxation time capillary time, serves critical parameter linking ink rheology printability. However, determining polymer-containing weakly viscoelastic fluids remains challenging due to need high-frequency measurements storage loss moduli. In this work, we present comprehensive analysis evaluate printability polymer-based fluids. Using squeeze-flow rheometer with piezoelectric axial vibration, measured properties inks up 104 Hz extracted elastic viscous coefficients. The Maxwell model was then employed simulate inks' behavior higher frequencies 106 rad·s−1 determine times consequently number. This performed varying polymer types concentrations, their jetting performances were examined using custom-built drop watcher system. By correlating printability, number–Weber (We) map, identifying optimal range 0.1 < De 1 2 We 15 stable single-drop jet formation. Our approach provides robust framework characterizing predicting various facilitating application advanced technologies.

Language: Английский

Citations

3
Evaluation of a bioprinted 3D airway tissue model for toxicity testing of nanomaterials; Pathway to integration into a tiered testing strategy for hazard assessment to support safety-by-design DOI Creative Commons
Yunji Lee,

Katie McAllister,

Hwa‐Rim Lee

et al.

Nano Today, Journal Year: 2025, Volume and Issue: 61, P. 102655 - 102655

Published: Feb. 3, 2025

Language: Английский

Citations

1
Development of a 3D bioengineered human lung submucosal gland ductal airway model to study mucociliary clearance in vitro DOI Creative Commons
Hoyeol Kim,

Sujung Yi,

Pramodha Liyanage

et al.

Published: March 1, 2025

Mucociliary clearance (MCC) is critical in maintaining lung health and preventing respiratory infections. MCC impaired people with cystic fibrosis, due to accumulation of thick, sticky mucus resulting from defective fibrosis transmembrane conductance regulator channel function. In this study, we developed a unique 3D submucosal gland ductal airway model utilizing primary human epithelial cells, which enables the formation physiologically relevant architecture epithelium including ciliary cells within bioprinted scaffold. Our observation demonstrates that not only fabrication airway-like structure mimicking vivo physiology, also facilitates quantitative measurement patient-specific determines pharmacological effects. results suggest could be valuable tool for understanding mechanisms underlying testing efficacy novel therapeutic strategies treatment diseases such as fibrosis.

Language: Английский

Citations

0
Construction of artificial lung tissue structure with 3D-inkjet bioprinting core for pulmonary disease evaluation DOI Creative Commons

Weimin Wan,

Xi Wang,

Rongtao Zhang

et al.

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: March 1, 2025

By integrating 3D-inkjet bioprinting technology, differentiated human cells can be assembled into artificial lung tissue structure to achieve a rapid, efficient, and reproducible disease model construction process. Here, we developed novel bioprinting-based method construct (ALTs) for acute injury (ALI) modeling, research application. It also used study the role of relevant in by adjusting cell type adapted bio-functions immune during cell-cell interactions. Firstly, series process optimizations were done mass-produce alginate hydrogel microspheres (Alg) with particle size 262.63 ± 5 μm using 3D bioprinter, then I collagen polydopamine deposited turns adhesion layer on surfaces Alg (P-Alg) was increased 328.41 3.81 μm. This platform exhibites good stability, timescale-dependent behavior, long-term adhesion. Subsequently, several including endothelial, epithelial, fibroblast, even such as macrophages adhered P-Alg through rotational culture, leading contractions aggregation, subsequently formed ALTs or (ALTs@M) alveolar-like structure. Finally, successfully constructed an ALI barrier damage lipopolysaccharide stimulation vitro, comparison secreted inflammatory factors between ALTs@M. Results demonstrated that ALTs@M more effective than stimulating microenvironment lungs, providing vitro cellular interactions macrophage research. Altogether, this strategy technology allowed flexible development structures potential models preclinical studies.

Language: Английский

Citations

0
Reinforcement Learning-Based Dynamic Optimization of Driving Waveforms for Inkjet Printing of Viscoelastic Fluids DOI
Seongju Kim, Minsu Cho, Sungjune Jung

et al.

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

In digital printing, the design and optimization of a driving waveform for piezoelectric printheads are critical precise patterning functional materials. This study introduces an approach using deep reinforcement learning (DRL) algorithm to dynamically control inks, which vary in properties with environmental conditions. We developed prediction model multilayer perceptron that accurately forecasts drop velocity jetting morphology based on ink's rheological parameters. Integrating this into DRL framework, we achieved over waveform, attaining target 3 ms-1 quantum dot ink within 20 steps. Further, implemented trained agent drop-watching system, enabling real-time adjustment maintain optimal despite changes due temperature variations. Our results demonstrate significant potential machine improving precision adaptability industrial inkjet printing processes.

Language: Английский

Citations

0
A ventilated perfused lung model platform to dissect the response of the lungs to viral infection DOI
İrem Deniz Derman,

Mecit Altan Alioglu,

Joseph Christakiran Moses

et al.

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Dynamic monitoring of a 3D-printed airway tissue model using an organic electrochemical transistor DOI

Seungjin Chai,

Yunji Lee, Róisı́n M. Owens

et al.

Biomaterials, Journal Year: 2024, Volume and Issue: 314, P. 122806 - 122806

Published: Sept. 2, 2024

Language: Английский

Citations

3
3D bioprinting of the airways and lungs for applications in tissue engineering and in vitro models DOI Creative Commons
Yanning Zhang, Yujian Liu, Chen Shu

et al.

Journal of Tissue Engineering, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 1, 2024

Tissue engineering and in vitro modeling of the airways lungs respiratory system are substantial research clinical importance. In airway lung models aim to improve treatment options for repair advance pathophysiological research. The construction biomimetic native with tissue-specific biological, mechanical, configurable features remains challenging. Bioprinting, an emerging 3D printing technology, is promising development airway, lung, disease models, allowing incorporation cells biologically active molecules into printed constructs a precise reproducible manner recreate airways, architecture, microenvironment. Herein, we present review bioprinting applications tissue modeling. key characteristics interstitium, alveoli described. bioinks recently used summarized. Furthermore, propose bioink categorization based on structural airways. Finally, challenges opportunities biofabrication discussed.

Language: Английский

Citations

0