Conjugation of Multiple Proteins Onto the Surface of PLGA/Lipid Hybrid Nanoparticles DOI Creative Commons
He Hu,

Chenming Zhang

Journal of Biomedical Materials Research Part A, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

ABSTRACT Nanoparticles are increasingly being used in the development of vaccines for disease prevention or treatment. Recent research has demonstrated that conjugating a protein onto surface nanoparticles can significantly increase its immunogenicity. Considering various pathogens threaten human health, multivalent often desirable. Up to now, nanoparticle‐based mostly limited one per nanoparticle. No been conducted explore possibility more than Here we developed specific conjugation strategy conjugate multiple proteins PLGA/lipid hybrid nanoparticle surface. The maleimide‐thiol Michael addition, Aizde‐DBCO (Dibenzocyclooctyne), and TCO (trans‐cycloctene)‐Tetrazine click chemistry were employed three different proteins, subunit keyhole limpet hemocyanin (sKLH), Ovalbumin (OVA), cross‐reactive material 197 (CRM ), (hNPs). successful results this study pave way developing against pathogens.

Language: Английский

Opioid use and abuse in adolescents and young adults; dealing with science, laws and ethics: Charming the COBRAS DOI

Donald E. Greydanus,

Ahsan Nazeer, Dilip R. Patel

et al.

Disease-a-Month, Journal Year: 2025, Volume and Issue: unknown, P. 101853 - 101853

Published: Jan. 1, 2025

Language: Английский

Citations

1
Conjugation of Multiple Proteins Onto the Surface of PLGA/Lipid Hybrid Nanoparticles DOI Creative Commons
He Hu,

Chenming Zhang

Journal of Biomedical Materials Research Part A, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

ABSTRACT Nanoparticles are increasingly being used in the development of vaccines for disease prevention or treatment. Recent research has demonstrated that conjugating a protein onto surface nanoparticles can significantly increase its immunogenicity. Considering various pathogens threaten human health, multivalent often desirable. Up to now, nanoparticle‐based mostly limited one per nanoparticle. No been conducted explore possibility more than Here we developed specific conjugation strategy conjugate multiple proteins PLGA/lipid hybrid nanoparticle surface. The maleimide‐thiol Michael addition, Aizde‐DBCO (Dibenzocyclooctyne), and TCO (trans‐cycloctene)‐Tetrazine click chemistry were employed three different proteins, subunit keyhole limpet hemocyanin (sKLH), Ovalbumin (OVA), cross‐reactive material 197 (CRM ), (hNPs). successful results this study pave way developing against pathogens.

Language: Английский

Citations

0