Application of a CYP1B1-Targeted NIR Probe for Breast Cancer Diagnosis, Surgical Navigation, and CYP1B1-Associated Chemotherapy Resistance Monitoring

Molecular Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Early detection and precise treatment for breast cancer are crucial, given its high global incidence rate. Hence, the development of novel imaging targets is essential diagnosing monitoring resistance to chemotherapy, which pivotal achieving personalized patients. In our previous work, we successfully developed a near-infrared (NIR) probe 1 CYP1B1-targeted imaging. this study, aimed investigate utility as NIR fluorescence photoacoustic dual-mode surveillance cancer. Western blotting cell lines has confirmed that CYP1B1 widely expressed in gynecological vitro capability tracking CYP1B1-positive tumor cells was validated by using confocal microscopy. Further studies, including vivo dual-model ex biological distribution analysis on triple-negative xenograft mouse model, demonstrated selectively accumulated tissue within early 0.5 h postinjection. The results surgical resection experiment revealed could be entirely removed under guidance imaging, thereby indicating probe's efficacy navigation. expression found upregulated adriamycin (ADR)-resistant cells, MCF-7/ADR. Consequently, sensitivity overexpressed MCF-7/1B1, ADR significantly reduced, with an IC50 value 0.586 ± 0.0934 μM, compared parental MCF-7 0.183 0.0444 μM. assays conducted MCF-7/ADR tumor-bearing mice specifically enriched sites, suggesting potential chemotherapy This study expands scope application 1, establishing diagnosis through fluorescence-photoacoustic resistance, resection. strategy paves way approaches

Language: Английский

Novel GAL7-targeted fluorescent molecular imaging probe for high-grade squamous intraepithelial lesion and cervical cancer screening

EJNMMI Research, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 13, 2025

Early detection and treatment are critical for improving the survival prognosis of patients with cervical cancer. However, there is a notable scarcity targeted imaging probes specifically designed to detect high-grade squamous intraepithelial lesions (HSIL) Our study aimed address this gap by identifying validating probe these conditions. Using bioinformatics data, we identified galectin-7 (GAL7) as highly expressed in cell carcinoma endocervical adenocarcinoma (CESC). Immunohistochemical staining biopsy samples from 30 HSIL cancer verified high specific expression GAL7. Further validation was performed using mouse human CESC lines tumor xenografts, confirming consistent Based on finding, synthesized GAL7-specific antibody conjugated FITC, creating GAL7-FITC fluorescence probe. Fluorescence molecular revealed that exhibited binding various xenograft models. Additionally, diagnostic capability demonstrated fresh specimens cone excisions, validated through histopathology immunohistochemical analysis. GAL7 target successfully developed has shown promising potential clinical application early CESC, providing rapid diagnosis without observable toxicity. This advancement may significantly enhance accuracy speed diagnostics, ultimately patient outcomes.

Language: Английский

808 nm Light-Triggered Cyanine-Decorated Iridium(III) Complexes for Antibacterial Photodynamic Therapy in Deep-Tissue

Inorganic Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Acute bacterial skin and structure infections (ABSSSIs) pose significant global health challenges, exacerbated by rising antibiotic resistance. Antibacterial photodynamic therapy (APDT) has emerged as a promising strategy to combat these utilizing photosensitizer (PS) that generates reactive oxygen species (ROS) upon light activation. However, the limited tissue penetration of conventional organic PSs, which primarily absorb in UV-vis spectra, hindered their therapeutic potential for deeper infections. Herein, we introduce novel iridium(III)-cyanine complex (Ir-cy) with strong near-infrared (NIR) absorption at 814 nm (up 101 red-shifted from previous reports), specifically designed enhance APDT. Under 808 laser irradiation, Ir-cy demonstrated substantial ROS generation capacity, achieving approximately 70% reduction Staphylococcus aureus (S. aureus) colonies depth 7.2 mm within simulated model. Comprehensive vitro vivo evaluations further confirmed its potent antibacterial efficacy against S. while maintaining excellent biocompatibility. These findings highlight highly effective NIR-active PS, paving way advanced strategies targeting deep-tissue ABSSSIs through optimized

Language: Английский