Triple-Functional Probiotics with Intracellularly Synthesized Selenium Nanoparticles for Colitis Therapy by Regulating the Macrophage Phenotype and Modulating Gut Microbiota DOI

Puze Li,

Lichong Zhu, Cheng Song

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

The dysregulated macrophage phenotype, as the main cause of colitis, not only enhanced oxidative stress to exacerbate inflammatory responses but was closely related with gut microbial dysbiosis. It needed simultaneously address three issues for effective treatment it satisfied. Here, we developed "three-birds-one-stone" probiotics, named Se@EcN-C2/A2, colitis treatment. Escherichia coli Nissle 1917 (EcN), a clinically approved probiotic, used intracellularly synthesize selenium (Se) nanoparticles by biomineralization, giving Se@EcN. Coating glycol chitosan and sodium alginate on surface Se@EcN (Se@EcN-C2/A2) endowed probiotics high resistance harsh gastrointestinal tract environment strong adhesion targeting ability inflamed site colon facilitate uptake M1 macrophages. Se@EcN-C2/A2 metabolized SeCys2 MetSeCys be involved in synthesis GPX2 TXNRD1, which led reaction oxygen species clearance inhibit Toll-like receptor nuclear factor κB signaling pathways suppress response polarize macrophages M2 phenotypes activating PI3K/AKT pathways. In DSS-induced mice, exerted satisfactory therapeutic prophylactic effects, including scavenging regulating restore barrier functions. Moreover, living probiotic EcN effectively regulated dysbiosis decreasing abundance Escherichia-Shigella increasing Lactobacillus Bifidobacterium.

Language: Английский

Selenium nanoparticles activate selenoproteins to mitigate septic lung injury through miR-20b-mediated RORγt/STAT3/Th17 axis inhibition and enhanced mitochondrial transfer in BMSCs DOI Creative Commons
Wan‐Jie Gu,

Fengzhi Zhao,

Wei Huang

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 20, 2025

Sepsis-induced acute lung injury (ALI) remains a critical clinical challenge with complex inflammatory pathogenesis. While bone marrow mesenchymal stem cells (BMSCs) demonstrate therapeutic potential through anti-inflammatory and cytoprotective effects, their age-related functional decline limits utility. This study developed chitosan-functionalized selenium nanoparticles (SeNPs@CS, 100 nm) to rejuvenate BMSCs miR-20b-mediated selenoprotein biosynthesis. Mechanistic investigations revealed that SeNPs@CS-treated exhibited enhanced mitochondrial transfer capacity, delivering mitochondria damaged alveolar epithelial (AECII) for cellular repair. Concurrently, miR-20b upregulation suppressed the RORγt/STAT3/Th17 axis, reducing pro-inflammatory Th17 cell differentiation in CD4+ T lymphocytes. The dual-target mechanism integrates immunomodulation via pathway inhibition rejuvenation therapy, representing paradigm-shifting approach ALI management. These engineered mitigated markers murine models, demonstrating superior efficacy conventional BMSC therapies. Our findings establish SeNPs@CS-modified as novel platform combining nanotechnology-enhanced engineering precision immunometabolic regulation, providing new avenues treatment of sepsis-induced ALI.

Language: Английский

Citations

0
Triple-Functional Probiotics with Intracellularly Synthesized Selenium Nanoparticles for Colitis Therapy by Regulating the Macrophage Phenotype and Modulating Gut Microbiota DOI

Puze Li,

Lichong Zhu, Cheng Song

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

The dysregulated macrophage phenotype, as the main cause of colitis, not only enhanced oxidative stress to exacerbate inflammatory responses but was closely related with gut microbial dysbiosis. It needed simultaneously address three issues for effective treatment it satisfied. Here, we developed "three-birds-one-stone" probiotics, named Se@EcN-C2/A2, colitis treatment. Escherichia coli Nissle 1917 (EcN), a clinically approved probiotic, used intracellularly synthesize selenium (Se) nanoparticles by biomineralization, giving Se@EcN. Coating glycol chitosan and sodium alginate on surface Se@EcN (Se@EcN-C2/A2) endowed probiotics high resistance harsh gastrointestinal tract environment strong adhesion targeting ability inflamed site colon facilitate uptake M1 macrophages. Se@EcN-C2/A2 metabolized SeCys2 MetSeCys be involved in synthesis GPX2 TXNRD1, which led reaction oxygen species clearance inhibit Toll-like receptor nuclear factor κB signaling pathways suppress response polarize macrophages M2 phenotypes activating PI3K/AKT pathways. In DSS-induced mice, exerted satisfactory therapeutic prophylactic effects, including scavenging regulating restore barrier functions. Moreover, living probiotic EcN effectively regulated dysbiosis decreasing abundance Escherichia-Shigella increasing Lactobacillus Bifidobacterium.

Language: Английский

Citations

0