Effects of the Tobacco Defensin NaD1 Against Susceptible and Resistant Strains of Candida albicans DOI Creative Commons
О. В. Шевченко, A.D. Voropaev, Ivan V. Bogdanov

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(12), P. 1092 - 1092

Published: Dec. 10, 2024

Today, Candida albicans is still the most common cause of both local and life-threatening systemic candidiasis. The spread resistant fungal strains has resulted in an urgent need to search for new promising antimycotics. Here, we investigated antifungal action tobacco defensin NaD1 against susceptible azoles echinocandins C. albicans. We demonstrated that was equally effective fungicidal all tested strains. MIC MFC values were 6.25 12.5 µM, respectively. showed first time could act synergistically not only with caspofungin but also human host defense antimicrobial peptides cathelicidin LL-37 β-defensin-2 (HBD2) Using flow cytometry, combinations or HBD2 can reinforce each other by enhancing membrane disruption. Caco-2 cell monolayer model, impaired adhesion cells epithelium. Moreover, inhibited formation biofilms Sabouraud broth less markedly nutrient-rich RPMI-1640 medium, enhanced antibiofilm activity caspofungin. Thus, hypothesized might affect development candidiasis vivo, including caused

Language: Английский

Enhancing antimicrobial activity through structural modification of Rip-thanatin with amino acid substitution DOI
Xuan Wu, Shaoyi Liu, Zongqiang Gong

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107501 - 107501

Published: March 1, 2025

Language: Английский

Citations

0

Advances in Artificially Designed Antibacterial Active Antimicrobial Peptides DOI
Ying Guo, Muhammad Haris Raza Farhan, Fei Gan

et al.

Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 22, 2024

ABSTRACT Antibacterial resistance has emerged as a significant global concern, necessitating the urgent development of new antibacterial drugs. Antimicrobial peptides (AMPs) are naturally occurring found in various organisms. Coupled with wide range activity, AMPs less likely to develop drug and can act potential agents for treating bacterial infections. However, their characteristics, such low instability, toxicity, hinder clinical application. Consequently, researchers inclined towards artificial design optimization based on natural AMPs. This review discusses research advancements field designing Moreover, it highlights strategies peptides, aiming provide valuable insights developing novel

Language: Английский

Citations

2

Propolis extract and Hermetia illucens larval proteins synergistically inhibit the growth of Aspergillus niger DOI
Qingzhi Ding, Zheng Yuan, Yongqi Zhu

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 61, P. 104661 - 104661

Published: July 5, 2024

Language: Английский

Citations

1

Stapled Peptides: An Innovative and Ultimate Future Drug Offering a Highly Powerful and Potent Therapeutic Alternative DOI Creative Commons
Do‐Hee Kim, Sung‐Min Kang

Biomimetics, Journal Year: 2024, Volume and Issue: 9(9), P. 537 - 537

Published: Sept. 5, 2024

Peptide-based therapeutics have traditionally faced challenges, including instability in the bloodstream and limited cell membrane permeability. However, recent advancements α-helix stapled peptide modification techniques rekindled interest their efficacy. Notably, these developments ensure a highly effective method for improving stability enhancing penetration. Particularly realm of antimicrobial peptides (AMPs), application has significantly increased been successfully applied to many peptides. Furthermore, constraining secondary structure also proven enhance biological activity. In this review, entire process through which hydrocarbon-stapled attain improved drug-like properties is examined. First, essential structural elements required activity as drugs are validated, specific residues identified using alanine scanning, stapling strategically incorporated at precise locations. Additionally, mechanisms by structure-based function AMPs explored, providing comprehensive engaging discussion.

Language: Английский

Citations

0

Effects of the Tobacco Defensin NaD1 Against Susceptible and Resistant Strains of Candida albicans DOI Creative Commons
О. В. Шевченко, A.D. Voropaev, Ivan V. Bogdanov

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(12), P. 1092 - 1092

Published: Dec. 10, 2024

Today, Candida albicans is still the most common cause of both local and life-threatening systemic candidiasis. The spread resistant fungal strains has resulted in an urgent need to search for new promising antimycotics. Here, we investigated antifungal action tobacco defensin NaD1 against susceptible azoles echinocandins C. albicans. We demonstrated that was equally effective fungicidal all tested strains. MIC MFC values were 6.25 12.5 µM, respectively. showed first time could act synergistically not only with caspofungin but also human host defense antimicrobial peptides cathelicidin LL-37 β-defensin-2 (HBD2) Using flow cytometry, combinations or HBD2 can reinforce each other by enhancing membrane disruption. Caco-2 cell monolayer model, impaired adhesion cells epithelium. Moreover, inhibited formation biofilms Sabouraud broth less markedly nutrient-rich RPMI-1640 medium, enhanced antibiofilm activity caspofungin. Thus, hypothesized might affect development candidiasis vivo, including caused

Language: Английский

Citations

0