Chemical Proteomics Reveals That Camptothecin Weakens Insect Immunity against Bacteria by Suppressing Antimicrobial Peptide Expression DOI
Huan Liu, Yi Ding,

Dongdong Huang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

Plant natural products are crucial in defending against herbivorous insects and widely used pest control, yet their mechanisms remain complex insufficiently studied. This study employed a reverse strategy to investigate the mechanism of camptothecin (CPT), botanical pesticide. By using CPT-based chemical probe coupled with proteomic analysis, immune-related proteins, including those involved prophenoloxidase (PPO) activation antimicrobial peptide (AMP) synthesis, were identified Asian corn borer, Ostrinia furnacalis. The findings demonstrated that CPT weakens insect immunity, primarily by inhibiting AMP synthesis rather than affecting PPO activation. Specifically, downregulated expression genes IMD pathway encoding AMPs (attacin gloverin). Additionally, CPT-fed exhibited reduced antibacterial activity. research uncovers novel as an immunosuppressant, offering new insights may enhance application control.

Language: Английский

Capsaicin from chili peppers and its analogues and their valued applications: An updated literature review DOI
Na Wang, Xiang Zhou,

Taihong Zhang

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 208, P. 116034 - 116034

Published: Feb. 25, 2025

Language: Английский

Citations

1
Competitive Affinity-Based Protein Profiling Reveals Potential Antifungal Targets of 1,2,3-Triazole Hydrazide in Fusarium graminearum DOI
Hao Liu,

Yu Jiang,

Jiahao Wang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

In previous research, 1,2,3-triazole hydrazide NAU-6ad exhibited remarkable broad-spectrum antifungal activity. However, the specific targets of remained unknown. Initially, we excluded three targets─succinate dehydrogenase, laccase, and ergosterol synthase─commonly associated with derivatives mentioned in literature. Subsequently, developed two types photoprobes: one incorporating diazirine (DA) other phenyl tetrazole (TZ), both featuring terminal alkynes for bioorthogonal reactions. Using these sets probes, a total 52 potential were identified through competitive affinity-based proteome profiling. Notably, Ndufs6 I1RC94 consistently by sets. The overexpression or knockout Ndufs6, subunit complex I, led to significant changes sensitivity F. graminearum. Similarly, subunits specifically Ndufs2, Ndufv1, Ndufa9, altered graminearum NAU-6ad, indicating that might act upon I. Further validation was provided enzyme activity tests, ATP content assays, pyruvate addition molecular docking, collectively reinforcing hypothesis function as I inhibitor.

Language: Английский

Citations

0
Decoding structural determinants of aryl hydrocarbon receptor antagonism by monoterpenoids DOI
Iveta Zůvalová, Barbora Vyhlídalová, Karolína Ondrová

et al.

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 157, P. 108265 - 108265

Published: Feb. 10, 2025

Language: Английский

Citations

0
Chemical Proteomics Reveals That Camptothecin Weakens Insect Immunity against Bacteria by Suppressing Antimicrobial Peptide Expression DOI
Huan Liu, Yi Ding,

Dongdong Huang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

Plant natural products are crucial in defending against herbivorous insects and widely used pest control, yet their mechanisms remain complex insufficiently studied. This study employed a reverse strategy to investigate the mechanism of camptothecin (CPT), botanical pesticide. By using CPT-based chemical probe coupled with proteomic analysis, immune-related proteins, including those involved prophenoloxidase (PPO) activation antimicrobial peptide (AMP) synthesis, were identified Asian corn borer, Ostrinia furnacalis. The findings demonstrated that CPT weakens insect immunity, primarily by inhibiting AMP synthesis rather than affecting PPO activation. Specifically, downregulated expression genes IMD pathway encoding AMPs (attacin gloverin). Additionally, CPT-fed exhibited reduced antibacterial activity. research uncovers novel as an immunosuppressant, offering new insights may enhance application control.

Language: Английский

Citations

0