Investigating the Interactions of Peptide Nucleic Acids with Multicomponent Peptide Hydrogels for the Advancement of Healthcare Technologies DOI Creative Commons
Silvana Giordano, Monica Terracciano, Enrico Gallo

et al.

Gels, Journal Year: 2025, Volume and Issue: 11(5), P. 367 - 367

Published: May 17, 2025

This study reports the development of peptide-based hydrogels for encapsulation and controlled release peptide nucleic acids in drug delivery applications. Ultrashort aromatic peptides, such as Fmoc-FF, self-assemble into biocompatible with nanostructured architectures. The functionalization tripeptides (Fmoc-FFK Fmoc-FFC) lysine (K) or cysteine (C) enables electrostatic covalent interactions model PNAs engineered glutamic acid residues, respectively. Hydrogels were polymerized situ presence PNAs, component ratios systematically varied to optimize mechanical properties, loading efficiency, kinetics. formulations obtained a 1/10 ratio Fmoc-FF(K C)/Fmoc-FF provided an optimal balance between structural integrity performance. All hydrogel demonstrated high stiffness (G′ > 19,000 Pa), excellent water retention, minimal swelling under physiological conditions (ΔW < 4%). studies over 10 days showed that enabled faster higher (up 90%), while bonding resulted slower, sustained (~15%). These findings highlight tunability system diverse therapeutic

Language: Английский

Investigating the Interactions of Peptide Nucleic Acids with Multicomponent Peptide Hydrogels for the Advancement of Healthcare Technologies DOI Creative Commons
Silvana Giordano, Monica Terracciano, Enrico Gallo

et al.

Gels, Journal Year: 2025, Volume and Issue: 11(5), P. 367 - 367

Published: May 17, 2025

This study reports the development of peptide-based hydrogels for encapsulation and controlled release peptide nucleic acids in drug delivery applications. Ultrashort aromatic peptides, such as Fmoc-FF, self-assemble into biocompatible with nanostructured architectures. The functionalization tripeptides (Fmoc-FFK Fmoc-FFC) lysine (K) or cysteine (C) enables electrostatic covalent interactions model PNAs engineered glutamic acid residues, respectively. Hydrogels were polymerized situ presence PNAs, component ratios systematically varied to optimize mechanical properties, loading efficiency, kinetics. formulations obtained a 1/10 ratio Fmoc-FF(K C)/Fmoc-FF provided an optimal balance between structural integrity performance. All hydrogel demonstrated high stiffness (G′ > 19,000 Pa), excellent water retention, minimal swelling under physiological conditions (ΔW < 4%). studies over 10 days showed that enabled faster higher (up 90%), while bonding resulted slower, sustained (~15%). These findings highlight tunability system diverse therapeutic

Language: Английский

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