Exosome-derived circ_0001785 delays atherogenesis through the ceRNA network mechanism of miR-513a-5p/TGFBR3

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Oct. 4, 2023

Abstract Purpose Endothelial cell dysfunction is a major cause of early atherosclerosis. Although the role extracellular vesicles in stabilizing atherosclerotic plaques well established, effect circulating exosomes on plaque formation still unknown. Here, we explored atherosclerosis based function that can act intercellular communication. Patients and methods We extracted serum from blood CHD patients (CHD-Exo) healthy individuals (Con-Exo). The obtained were co-cultured with human umbilical vein endothelial cells (HUVECs) vitro. In addition, determined circ_0001785 functions as competing endogenous RNA (ceRNAs) coronary artery disease by dual luciferase reporter gene analysis. protective against injury was also verified using over-expression lentiviral transfection functional assays. vivo experiments, injected over-expressed lentivirus into tail mice to observe its therapeutic mouse model Results vitro results showed amount plasma-derived have an increase disease, inflammation apoptosis exacerbated. Over-expression reduced through ceRNA network pathway miR-513a-5p/TGFBR3. Quantitative reverse transcription-polymerase chain reaction identified expressed peripheral increased within tissue. experiments aortic intraplaque neo-vascularization, enhanced left ventricular diastolic function, thereby delaying development mice. Conclusion Our demonstrated new biomarker, exosome-derived circ_0001785, for atherogenesis, which reduce thus delay atherogenesis miR-513a-5p/TGFBR3 mechanism, providing exosome-based intervention strategy

Language: Английский

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Functions and application of circRNAs in vascular aging and aging-related vascular diseases

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 17, 2025

Circular RNAs (circRNAs), constituting a novel class of endogenous non-coding generated through the reverse splicing mRNA precursors, possess capacity to regulate gene transcription and translation. Recently, pivotal role circRNAs in controlling vascular aging, as well pathogenesis progression aging-related diseases, has garnered substantial attention. Vascular aging plays crucial increased morbidity mortality elderly. Endothelial cells (ECs) smooth muscle (VSMCs) are components intima media layers wall, respectively, closely involved mechanisms underlying diseases. The review aims provide comprehensive exploration connection between Besides, circRNAs, potential diagnostic markers or therapeutic targets for will be discussed thoroughly, along with challenges limitations their clinical application. Investigating molecular diseases insight into early diagnosis therapy, even effective prognosis assessment these conditions.

Language: Английский

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Circ-SATB2 upregulates STIM1 expression and regulates vascular smooth muscle cell proliferation and differentiation through miR-939

Biochemical and Biophysical Research Communications, Journal Year: 2018, Volume and Issue: 505(1), P. 119 - 125

Published: Sept. 18, 2018

Language: Английский

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Deep Insights in Circular RNAs: from biogenesis to therapeutics

Biological Procedures Online, Journal Year: 2020, Volume and Issue: 22(1)

Published: May 14, 2020

Circular RNAs (circRNAs) have emerged as a universal novel class of eukaryotic non-coding RNA (ncRNA) molecules and are becoming new research hotspot in biology. They form covalent loop without 5' cap 3' tail, unlike their linear counterparts. Endogenous circRNAs mammalian cells abundantly conserved discovered so far. In the biogenesis exonic, intronic, reverse complementary sequences or RNA-binding proteins (RBPs) play very important role. Interestingly, majority them highly conserved, stable, resistant to RNase R show developmental-stage/tissue-specific expression. CircRNAs multifunctional roles microRNA (miRNA) sponges, regulators transcription post-transcription, parental gene expression translation various diseased conditions. Growing evidence shows that an role neurological disorders, atherosclerotic vascular disease, cancer potentially serve diagnostic predictive biomarkers due its abundance biological samples. Here, we review biogenesis, properties, functions, impact on diseases.

Language: Английский

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The Role of Circular RNAs in Cerebral Ischemic Diseases: Ischemic Stroke and Cerebral Ischemia/Reperfusion Injury

Advances in experimental medicine and biology, Journal Year: 2018, Volume and Issue: unknown, P. 309 - 325

Published: Jan. 1, 2018

Language: Английский

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