Dynamic of SARS-CoV-2 variants circulation in Tunisian pediatric population, during successive waves, from March 2020 to September 2022

Virus Research, Journal Year: 2024, Volume and Issue: 344, P. 199353 - 199353

Published: March 20, 2024

The emergence of SARS-CoV-2 variants has led to several cases among children. However, limited information is available from North African countries. This study describes the strains circulating in Tunisian pediatric population during successive waves. A total 447 complete sequences were obtained individuals aged 13 days 18 years, between March 2020 and September 2022: 369 generated this 78 ones, GISAID, previously patients. These compared with 354 274 ones adults a global dataset, respectively. variant circulation dynamics predominant investigated period using maximum-likelihood phylogenetic analysis. Among studied population, adolescents age group, comprising 55.26% cases. Twenty-three lineages identified; seven which not reported Tunisia. Phylogenetic analysis showed close relationship connections other countries variable according variants: relationships observed for Alpha, B1.160 Omicron variants, while independent clusters Delta B.1.177 lineages. findings highlight pivotal role children virus transmission underscore impact vaccination on spread. Vaccination children, booster doses, may be considered better management future emergences.

Language: Английский

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Exploiting the Electrostatic Binding of Ruthenium Hexamine Molecular Redox Nanowires onto DNA/OGCN Biohybrid Electrodes toward the Electrochemical Detection of COVID-19

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

The Coronavirus Disease 2019 (COVID-19) recently emerged as a life-threatening global pandemic that has ravaged millions of lives. affected patients are known to frequently register numerous comorbidities induced by COVID-19 such diabetes, asthma, cardiac arrest, hypertension, and neurodegenerative diseases, name few. expensiveness probability false negative results conventional screening tests often delay timely diagnosis treatment. In cases, the deployment suitable biosensing platform can readily expedite rapid process for enhanced patient outcomes. We report development an electrochemical genosensor based on DNA/OGCN (DNA/oxygenated graphitic carbon nitride) nanohybrids quantification severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) DNA─the key biomarker COVID-19. This is achieved exploiting molecular nanowire-formation capability [Ru(NH3)6]2+/3+ redox probe onto DNA phosphate backbone via electrostatic interactions. microstructural characterization OGCN was performed using scanning electron microscopy (SEM) coupled with energy-dispersive X-ray (EDX) module, diffraction (XRD), Fourier transform infrared spectroscopy. analyses were cyclic voltammetry (CV) impedance spectroscopy (EIS), while analytical performance sensor evaluated square wave (SWV). developed exhibited wide linear detection range within 10 fM–10 μM, limit (LoD) ∼7.23 fM high degree selectivity toward SARS-CoV-2 target DNA, thereby indicating its potential be employed in point-of-care scenario providing affordable healthcare populace.

Language: Английский

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Insights into Omicron: Genomic Characterization and Inpatient Risk Assessment at Single Tertiary Hospital in Indonesia

Journal of Multidisciplinary Healthcare, Journal Year: 2025, Volume and Issue: Volume 18, P. 71 - 81

Published: Jan. 1, 2025

Purpose: Omicron is a variant with the highest number of mutations among all Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) viruses, making whole genome sequencing (WGS) an essential tool for public health surveillance and molecular epidemiology. It important to note that data can provide insights into virus evolution disease control. This study aims overview WGS results SARS-CoV-2 Variant at Hasan Sadikin General Hospital Bandung. Patients Methods: was conducted using analytical observational method. Data collected retrospectively from medical records, reverse transcriptase polymerase chain reaction (RT-PCR) results, patients Bandung July December 2022, who met inclusion criteria. The lineage trends, mutation profiles, characteristics differences, factors influencing hospitalization were also described. Results: Among 239 subjects, 50 lineages identified, BA.5.2 (28%) XBB.1 (19.2%) dominating since October respectively. spike gene had frequency, accounting 28.8% out 532 types identified. In lineage, 97.01, 92.53, 100% L452R mutation, F486V H69/V70 deletion, R346T N460K mutations, no deletion observed. associated 5.49 times greater risk inpatient treatment (95% CI: 1.73– 17.38) compared BA.5.2, while adjusted odds ratio (aOR) vaccinations 0.45 0.29– 0.7). Conclusion: dominated infections most occurring in gene. Inpatient influenced by type showing higher risk. A significantly reduced this risk, emphasizing protective role vaccination. Keywords: SARS-CoV-2, variant,

Language: Английский

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Decoding SARS-CoV-2 variants: Mutations, viral stability, and breakthroughs in vaccines and therapies

Biophysical Chemistry, Journal Year: 2025, Volume and Issue: 320-321, P. 107413 - 107413

Published: Feb. 20, 2025

Language: Английский

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The comprehensive insights into the B-cells-mediated immune response against COVID-19 infection amid the ongoing evolution of SARS-CoV-2

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 185, P. 117936 - 117936

Published: March 8, 2025

Language: Английский

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