Pan-cancer and multi-omics analyses revealed the diagnostic and prognostic value of BAZ2A in liver cancer DOI Creative Commons
Yan Liu, Junli Wang, Jimin Guo

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 14, 2023

Abstract BAZ2A, an epigenetic regulatory factor that affects ribosomal RNA transcription, has been shown to be highly expressed in several cancers and promotes tumor cell migration. This study explored the expression mechanism of BAZ2A tumorigenesis at pan-cancer level. The Cancer Genome Atlas, Gene Expression Omnibus databases TIMER2.0, cBioPortal other tools were used analyze level various tissues examine relationship between survival, prognosis, mutation immune invasion. In vitro experiments performed assess function cancer cells. Using combined transcriptome proteome analysis, we examined possible tumors. exhibited high levels multiple displayed a significant association with patient prognosis. main type genetic variation is gene mutation. Downregulation inhibited proliferation, migration, invasion promoted apoptosis LM6 liver development may involve lipid metabolism. These results help expand our understanding suggest serve as prognostic diagnostic cancers.

Language: Английский

The tale of SOX2: Focusing on lncRNA regulation in cancer progression and therapy DOI
Peng Huang,

Feng Wen,

YiShan Li

et al.

Life Sciences, Journal Year: 2024, Volume and Issue: 344, P. 122576 - 122576

Published: March 14, 2024

Language: Английский

Citations

9
XIST and MUC1-C form an auto-regulatory pathway in driving cancer progression DOI Creative Commons

Keyi Wang,

Atrayee Bhattacharya, Naoki Haratake

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(5)

Published: May 13, 2024

Abstract The long non-coding RNA X-inactive specific transcript (lncRNA XIST) and MUC1 gene are dysregulated in chronic inflammation cancer; however, there is no known interaction of their functions. present studies demonstrate that MUC1-C regulates XIST lncRNA levels by suppressing the RBM15/B, WTAP METTL3/14 components m6A methylation complex associate with A repeats. also suppresses YTHDF2-CNOT1 deadenylase recognizes sites contributes to decay increases stability expression. In support an auto-regulatory pathway, we show expression promoting NF-κB-mediated activation gene. Of significance, regulate common genes associated stemness, including (i) miR-21 which upregulated across pan-cancers, (ii) TDP-43 associates E Our results further MUC1-C/XIST pathway regulated TDP-43, drives stemness-associated genes, (iii) necessary for self-renewal capacity. These findings indicate axis importance cancer progression.

Language: Английский

Citations

6
ASCL1-mediated ferroptosis resistance enhances the progress of castration-resistant prostate cancer to neurosecretory prostate cancer DOI Open Access

Jiawei Nie,

Peng Zhang,

Chaoqi Liang

et al.

Free Radical Biology and Medicine, Journal Year: 2023, Volume and Issue: 205, P. 318 - 331

Published: June 23, 2023

Language: Английский

Citations

16
Huang-qin decoction increases the sensitivity of EGFR-TKIs to NSCLC cells by regulating stat3/GPX4 to induce redox ratio and ROS to inhibit CSCs DOI Creative Commons
Yaya Yu,

Chenjing Lei,

Yanan Li

et al.

Journal of Traditional and Complementary Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Mouse Model Study: Early Life Chronic Stress Effects on Sox2 and Bcl2 mRNA Expression in Gastrointestinal Tissues DOI Open Access
Keziban Korkmaz Bayramov, Aida Nurul Barokah, Merve Hilal Dönmez

et al.

Meandros Medical And Dental Journal, Journal Year: 2025, Volume and Issue: 26(1), P. 42 - 48

Published: March 20, 2025

Objective: Early-life chronic stress can impact the gastrointestinal (GI) tract and increase cancer risk. Studies on mouse models have shown that maternal cause lasting changes in offspring's physiology behaviour. These be observed GI tract, where disturbances cellular processes, such as apoptosis, occur. This study examined mRNA expression tissues of maternally stressed mice, focusing Sox2 Bcl2 expressions. Materials Methods: Pregnant Balb/c mice were randomly divided into three groups. The litters control exposed to routine conditions. In contrast, others unpredictable separation (MS) for hours every day between 1-14 postnatal days (PND). Half MS dams (MSUS) within these hours. Five-week-old sacrificed, total RNA was isolated from muscle, duodenum, stomach using Phenol-Chloroform technique. Sox2, Gapdh, measured by Rotor-Gene Q. data obtained analysed One-Way ANOVA tests Kruskal-Wallis GraphPad Prism9. Results: Although remained unchanged, it significantly increased duodenum (p=0.0132). Similarly, while muscle did not change substantially, gastric tissue MSUS (p=0.0030). Furthermore, a significant positive correlation found genes (p=0.005). Conclusion: Early life stress, dysfunction, susceptibility may intricately linked. Understanding molecular mechanisms involved new implications developing interventions reduce risk cancer. research also provide insights strategies treating predisposed individuals.

Language: Английский

Citations

0
Design, synthesis, and biological evaluation of quinolinyl-ureido-phenyl-hydrazide derivatives and quinolinyl-hydrazide derivatives as anticancer agents targeting Nur77-mediated ferroptosis DOI
Yanfang Gao, Yilong Yang, Jingbo Qin

et al.

European Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 117559 - 117559

Published: April 1, 2025

Language: Английский

Citations

0
High expression of BCAT1 sensitizes AML cells to PARP inhibitor by suppressing DNA damage response DOI Open Access

Jiajia Pan,

Yungui Wang,

Shujuan Huang

et al.

Journal of Molecular Medicine, Journal Year: 2024, Volume and Issue: 102(3), P. 415 - 433

Published: Feb. 10, 2024

Language: Английский

Citations

3
Comprehensive analysis of ferroptosis-related genes reveals potential therapeutic targets in osteoporosis patients: a computational analysis and in vitro experiments DOI Creative Commons
Sihui Chen, Yiyi Jiang, Gengqiang Xie

et al.

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 10, 2025

Ferroptosis-related genes have been reported to play important roles in many diseases, but their molecular mechanisms osteoporosis not elucidated. Based on two independent GEO datasets (GSE35956 and GSE35958), GSE35959 as the validation dataset, we comprehensively elucidated pathological mechanism of ferroptosis-related by GO analyses, KEGG analyses a PPI network. Then, We used Western Blot (WB) Quantitative real-time polymerase chain reaction (qPCR) verify expression level KMT2D, hub gene, clinical samples. Subsequently, predicted upstream miRNA KMT2D gene analyzed osteoporosis, potential prognostic value its immune invasion pan-cancer. This study identified MYCN, TP63, RELA, SOX2, CDKN1A key osteoporotic cell aging. The dataset validated that was significantly upregulated experimental verification results qPCR WB indicate is highly expressed patients with osteoporosis. Further analysis revealed hsa-miR-204-5p-KMT2D axis may an role aging cells. reveals mainly cells through epigenetics provides theoretical basis for treatment

Language: Английский

Citations

0
Breast cancer patient-derived scaffolds enhance the understanding of PD-L1 regulation and T cell cytotoxicity DOI Creative Commons

Elena Garré,

Sara Rhost,

Anna Gustafsson

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: April 16, 2025

Abstract Recent advances as well obstacles for immune-based cancer treatment strategies, highlight the notable impact of patient microenvironments on immune cells and targets. Here, we use patient-derived scaffolds (PDS) generated from 110 primary breast cancers to monitor microenvironment regulators. Pronounced variation in PD-L1 expression is observed adapted different scaffolds. This further linked clinical observations correlated with specific proteins detected cell-free PDSs using mass spectrometry. When adding T PDS-based cultures, killing efficiency activated vary between whereas non-activated modulate cell treatment-predictive values, matching capacities cells. Surviving show enrichment stem epithelial-to-mesenchymal transition (EMT) features, suggesting that may not efficiently target metastatic potential. We conclude clinically relevant insights how optimally guide therapies can be obtained by including cues handling drug development.

Language: Английский

Citations

0
AKT1 as a therapeutic target for platinum-resistant SOX2 positive ovarian cancer cells DOI Creative Commons
Mengyang Xue, Kang Li, Yunfeng Zhang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 29, 2025

Ovarian cancer remains the most lethal gynecological malignancy, largely owing to its chemotherapy resistance and high recurrence rate. Emerging evidence has linked aberrant expression of SOX2, a transcription factor that is important in development maintenance stem cell state, with chemoresistance poor prognosis ovarian patients. In this study, we aimed elucidate mechanisms drive SOX2 cells. By examining multiple lines panel clinical tumor samples, observed broad overexpression tumors. To identify signaling pathway(s) drives cells, screened set small-molecule kinase inhibitors target 30 major cellular kinases. Among top hits identified are AKT inhibitors. We demonstrated inhibition or knockdown AKT1 can drastically downregulate protein level, impairs growth stemness SOX2-positive markedly sensitize cells platinum drugs. Mechanically, found primarily by enhancing stability does so phosphorylating at threonine 116. Altogether, our study reveals an underlying mechanism underscores pharmacological as potential therapeutic strategy

Language: Английский

Citations

0