Curcumin modulated gut microbiota and alleviated renal fibrosis in 5/6 nephrectomy-induced chronic kidney disease rats

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0314029 - e0314029

Published: Jan. 9, 2025

Increasing evidence suggests that dysbiosis of gut microbiota exacerbates chronic kidney disease (CKD) progression. Curcumin (CUR) has been reported to alleviate renal fibrosis in animal models CKD. However, the relationship between CUR and microbiome CKD remains unclear. This study aims investigate potential anti-renal effects from perspective. A 5/6 nephrectomy (5/6Nx) rat model was used explore therapeutic effect on fibrosis. Tight junction protein expression levels were measured assess intestinal barrier function. 16S rRNA sequencing employed evaluate changes composition, metabolomics utilized detect alterations plasma metabolites. The administration significantly ameliorated inhibited inflammation 5/6Nx rats. Additionally, markedly improved tight proteins local colon inflammation. also positively reconstructed microbiota, increasing abundance beneficial bacteria, such as Lachnospiraceae_NK4A136_group , Eubacterium_siraeum_group Muribaculaceae increased. Metabolomics revealed reduced uremic retention solutes elevated Vitamin D short-chain fatty acids (SCFAs). Spearman correlation analysis indicated genera enriched by correlated with SCFA negatively injury biomarkers. Mechanistically, we found inhibition LPS/TLR4/NF-κB TGF-β1/Smads pathway CUR-treated Our indicates modulate this modulation may contribute anti-fibrosis CUR.

Language: Английский

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Uremic toxins mediate kidney diseases: the role of aryl hydrocarbon receptor

Cellular & Molecular Biology Letters, Journal Year: 2024, Volume and Issue: 29(1)

Published: March 15, 2024

Abstract Aryl hydrocarbon receptor (AhR) was originally identified as an environmental sensor that responds to pollutants. Subsequent research has revealed AhR recognizes multiple exogenous and endogenous molecules, including uremic toxins retained in the body due decline renal function. Therefore, is also considered be a toxin receptor. As ligand-activated transcriptional factor, activation of involved cell differentiation senescence, lipid metabolism fibrogenesis. The accumulation hazardous all tissues organs. identification opens door investigating precise role molecular mechanism tissue organ damage induced by toxins. This review focuses on summarizing recent findings chronic kidney disease, diabetic nephropathy acute injury. Furthermore, potential clinical approaches mitigate effects are explored herein, such enhancing clearance through dialysis, reducing production dietary interventions or microbial manipulation, manipulating metabolic pathways controlling signaling. information may shed light toxin-induced injury other organs, provide insights into manipulate accumulated Graphical

Language: Английский

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Butyrate promotes kidney resilience through a coordinated kidney protective response in tubular cells

Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: 224, P. 116203 - 116203

Published: April 12, 2024

Acute kidney injury (AKI) is common in hospitalized patients and increases short-term long-term mortality. Treatment options for AKI are limited. Gut microbiota products such as the short-chain fatty acid butyrate have anti-inflammatory actions that may protect tissues, including kidney, from injury. However, molecular mechanisms of tissue protection by poorly understood. with oral two weeks prior to folic acid-induced during improved function decreased tubular inflammation while stopping before was not protective. Continuous preserved expression protective factors Klotho, PGC-1α Nlrp6 which were otherwise downregulated. In cultured cells, blunted maladaptive cell response a proinflammatory milieu, preserving factors. Kidney afforded this continuous schedule confirmed second model nephrotoxic AKI, cisplatin nephrotoxicity, where also preserved. To assess contribution preservation resilience, recombinant Klotho administered mice cisplatin-AKI shown preserve Nlrp6, decrease AKI. conclusion, promotes resilience decreases preventing downregulation genes Klotho. This information be relevant optimize antibiotic management hospitalization.

Language: Английский

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Chia (Salvia hispanica L.) flour modulates the intestinal microbiota in Wistar rats fed a high-fat and high-fructose diet

Food Research International, Journal Year: 2023, Volume and Issue: 172, P. 113095 - 113095

Published: June 10, 2023

Language: Английский

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The AKI-to-CKD Transition: The Role of Uremic Toxins

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(22), P. 16152 - 16152

Published: Nov. 10, 2023

After acute kidney injury (AKI), renal function continues to deteriorate in some patients. In a pro-inflammatory and profibrotic environment, the proximal tubules are subject maladaptive repair. AKI-to-CKD transition, impaired recovery from AKI reduces tubular glomerular filtration leads chronic disease (CKD). Reduced secretion capacity is characterized by plasma accumulation of biologically active molecules, referred as uremic toxins (UTs). These have role development neurological, cardiovascular, bone, complications CKD. However, UTs might also cause CKD well be consequence. Recent studies shown that these molecules accumulate early contribute establishment this environment kidney. The objective present work was review mechanisms UT toxicity potentially transition each compartment.

Language: Английский

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Gut Microbiota Interventions to Retain Residual Kidney Function

Toxins, Journal Year: 2023, Volume and Issue: 15(8), P. 499 - 499

Published: Aug. 11, 2023

Residual kidney function for patients with chronic disease (CKD) is associated better quality of life and outcome; thus, strategies should be implemented to preserve function. Among the multiple causes that promote damage, gut dysbiosis due increased uremic toxin production endotoxemia need attention. Several have been proposed modulate microbiota in these patients, diet has gained increasing attention recent years since it primary driver dysbiosis. In addition, medications faecal transplantation may valid strategies. Modifying composition mitigate damage residual Although various studies shown influential role modulating composition, effects this modulation on remain limited. This review discusses metabolism vice versa how we could by balance.

Language: Английский

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Gut microbiota mediates the protective effects of β-hydroxybutyrate against cisplatin-induced acute kidney injury

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 175, P. 116752 - 116752

Published: May 17, 2024

The gut microbiota has been reported to be perturbed by chemotherapeutic agents and modulate side effects. However, the critical role of β-hydroxybutyrate (BHB) in regulation pathogenesis related nephrotoxicity remains unknown. We conducted a comparative analysis composition function healthy, cisplatin-challenged, BHB-treated, high-fat diet-treated mice using 16 S rDNA gene sequencing. To understand crucial involvement intestinal flora BHB's cisplatin -induced nephrotoxicity, we administered antibiotics deplete performed fecal transplantation (FMT) before administration. sequencing demonstrated that both endogenous exogenous BHB restored dysbiosis cisplatin-induced barrier disruption mice. Additionally, our findings suggested LPS/TLR4/NF-κB pathway was responsible for triggering renal inflammation gut-kidney axis. Furthermore, ablation eliminated renoprotective effects against acute kidney injury. FMT also confirmed administration BHB-treated provided protection nephrotoxicity. This study elucidated mechanism which affects mediation inhibiting inflammatory response, may help develop novel therapeutic approaches target microbiota.

Language: Английский

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Probiotic Kefir Improves Renal Disorders in Ovariectomized Female SHR with High Fructose Intake–Induced Metabolic Syndrome

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract Women in postmenopausal period may present several comorbidities linked to metabolic syndrome (MetS). Our hypothesis is that kefir prevent the deleterious effects renal function an experimental model of (MetS) and ovarian hormone deficiency. Young female spontaneously hypertensive rats (SHR) were divided into four groups: ovariectomized (OVX) control, OVX fructose, kefir, + fructose. They received (5% w/v) via gavage for 8 weeks, while fructose (10% was available ad libitum. In ponderal parameters glucose metabolism, we observe fructose-overloaded groups (OF OKF) showed increased weight, visceral fat, fasting blood glucose. However, OKF partially reduced glycemic peak tolerance test. Moreover, standard method measurement OF had a reduction glomerular filtration rate, surprisingly exhibited flow (RBF RPF) decreased resistance (RVR). These might be associated with findings oxidative stress nitric oxide (NO) bioavailability, which group capable increasing total nitrogen oxides (NOx), attenuate generation hydrogen peroxide (DCF) peroxynitrite (HPF), also elevated microalbuminuria promoted by even though systemic pressure between did not differ. Taking together our results, study, favorable deficiency (OKF), potentially protecting kidney from

Language: Английский

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Positive pathogens in stool could predict the clinical outcomes of sepsis-associated acute kidney injury in critical ill patient

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 16, 2024

In this study, we sought to evaluate the influence of positive pathogens in stool (PPS) on clinical outcomes critical ill patients with Sepsis-associated acute kidney injury (S-AKI) from intensive care unit. Our sample consisted 7338 patients, whom 752 (10.25%) had PPS. We found that presence Clostridium difficile (C. difficile) and protists samples was correlated survival during hospitalization, as well 30-day 90-day survival. Interestingly, there no significant difference overall in-hospital between PPS group negative (NPS) control group. However, cumulative incidence infection-related mortality significantly higher (53 vs. 48%, P = 0.022), particularly C. their specimens. After adjusting for propensity scores, results also have statistical significance. These findings suggest may affect 90-days S-AKI, samples. Further research is warranted further explore these associations.

Language: Английский

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5-aminolaevulinic acid with sodium ferrous citrate alleviated kidney injury and fibrosis in a unilateral ureteral obstruction model

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 150, P. 114321 - 114321

Published: Feb. 18, 2025

Language: Английский

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