Evaluation of purine-nucleoside degrading ability andin vivouric acid lowering ofStreptococcus thermophilus(ID-PDP3), a novel antiuricemia strain DOI Creative Commons
Dayoung Kim, Jin Seok Moon, Ji Eun Kim

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 12, 2023

Abstract This study evaluated 15 lactic acid bacteria in terms of their ability to degrade inosine and hypoxanthine—which are the intermediates purine metabolism—for management hyperuricemia gout. After a preliminary screening based on HPLC, CR1 ( Lactiplantibacillus plantarum ) GZ1 pentosus showed highest nucleoside degrading rates were therefore selected for further characterization. IDCC 2201 S. thermophilus ), which possessed hpt gene encoding hypoxanthine-guanine phosphoribosyltransferase (HGPRT) exhibited degradation, was also selected. These three strains examined effect probiotics lowering serum uric rat model potassium oxonate (PO)-induced hyperuricemia. Among them, level most reduced by (p < 0.05). Further, analysis microbiome that administration recovered ratio Bacteroidetes/Firmicutes intestinal microbial composition unbalanced difference compared group administered with allopurinol. Moreover, short-chain fatty acids (SCFAs) significantly increased. Ultimately, findings show lowers levels purine-nucleosides restores flora SCFAs, ultimately suggesting is promising candidate use as an adjuvant treatment patients

Language: Английский

Hyperuricemia research progress in model construction and traditional Chinese medicine interventions DOI Creative Commons
Hongyan Zhou, Jingyi Yang, Xiao Yuan

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: March 7, 2024

Hyperuricemia (HUA), a severe metabolic disease derived from purine metabolism disorder, will lead to abnormally increased serum uric acid (SUA) levels in the body. Studies have shown that HUA is highly related gout, hypertension, diabetes, coronary heart disease, chronic kidney diseases, and so on. Traditional Chinese medicine (TCM) shows excellent results treating because of its unique advantages multi-metabolites multi-targets. This article reports on use TCM components for (UA)-lowering activity with efficacy low side effects based established models. work summarizes limitations various models evaluation. Applications treatment also been discussed detail. paper reveals recent research progress constructing evaluation systematic interventions. It provide scientific reference establishing model suggest future TCM-related studies.

Language: Английский

Citations

17
Echinacea purpurea polysaccharide intervene in hepatocellular carcinoma via modulation of gut microbiota to inhibit TLR4/NF-κB pathway DOI

Gaoxiang Jing,

Wenqian Xu, Wei Ma

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 261, P. 129917 - 129917

Published: Feb. 2, 2024

Language: Английский

Citations

14
Structure–function relationship of the brown seaweed Undaria pinnatifida laminaran: Protein kinase C-mediated mucus secretion and gut barrier restoration DOI
Kit‐Leong Cheong, Lin Chen, Siyuan Lu

et al.

Carbohydrate Polymers, Journal Year: 2025, Volume and Issue: unknown, P. 123525 - 123525

Published: March 1, 2025

Language: Английский

Citations

1
Limosilactobacillus reuteri HCS02-001 Attenuates Hyperuricemia through Gut Microbiota-Dependent Regulation of Uric Acid Biosynthesis and Excretion DOI Creative Commons
Akbar Hussain, Binqi Rui,

Hayan Ullah

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(4), P. 637 - 637

Published: March 22, 2024

Hyperuricemia is a prevalent metabolic disorder that arises from abnormal purine metabolism and reduced excretion of uric acid (UA). The gut microbiota plays significant role in the biosynthesis UA. Probiotics capable degradation possess potential to prevent hyperuricemia. Our study aimed screen probiotics areas with abundant dairy products longevity populations China, which could attenuate level UA explore underlying mechanism. In this study, twenty-three lactic bacteria isolated healthy Chinese infant feces traditional fermented foods such as hurood lump milk were evaluated for ability tolerance acid, bile, artificial gastric juice, intestinal juice determine candidate strains probiotics. Eight identified possessing superior simulated conditions further analyzed by high-performance liquid chromatography (HPLC), revealing Limosilactobacillus reuteri HCS02-001 (Lact-1) Lacticaseibacillus paracasei HCS17-040 (Lact-2) most potent degrade nucleosides. effect Lact-1 Lact-2 on hyperuricemia was intervening them potassium oxonate adenine-induced Balb/c mice model vivo. results showed serum hyperuricemic can be efficiently via oral administration (p < 0.05). It significantly inhibited levels liver inflammatory cytokines hepatic xanthine oxidase through TLR4/MyD88/NF-κB pathway across gut–liver axis. Furthermore, transporters ABCG2 SLC2A9 substantially upregulated intervention probiotic. Fecal ATP induced, while fecal dehydrogenase allantoinase increased following RNA sequencing HT-29 cells line treated its metabolites demonstrated regulation pathways related summary, these findings demonstrate possesses capacity ameliorate inhibiting enhancing gastrointestinal barrier functions promoting removal upregulation urate transporters, thereby providing basis probiotic formulation targeting microbiota.

Language: Английский

Citations

5
Imperata cylindrica polysaccharide ameliorates intestinal dysbiosis and damage in hyperuricemic nephropathy DOI

Wenchen Yu,

Gang Huang, Junwen Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134432 - 134432

Published: Aug. 7, 2024

Language: Английский

Citations

4
Mechanisms Associated With Renal Injury in Hyperuricemia and Strategies for the Development of Natural Active Substances DOI Open Access

Wanping Lv,

Huixiang Chen, Pan Zhou

et al.

International Journal of Rheumatic Diseases, Journal Year: 2025, Volume and Issue: 28(2)

Published: Feb. 1, 2025

Hyperuricemia (HUA) is a metabolic condition resulting from an abnormality in the process of purine metabolism. Its occurrence has been on rise globally. The results relevant studies show that 5% to 12% HUA patients will eventually develop gout, and one-third these may involve kidneys kidney disease. Although severe renal health hazards associated with excessive uric acid levels are well known, specific molecular mechanisms remain unknown. Therefore, this paper provides insights into related chain reactions leading injury three perspectives: imbalance intestinal homeostasis, oxidative stress response, NLRP3 inflammasome. In addition, standing against background strong side effects high tolerability disadvantages commercially available acid-lowering drugs such as allopurinol, benzbromarone, febuxostat, development new active anti-hyperuricemic drug fewer justified. This article reviews progress research natural actives (probiotics, dietary polyphenols, peptides) safety profile, multi-targeting, integrative modulatory effects, attempt provide some ideas for developers.

Language: Английский

Citations

0
Sodium Butyrate Alleviates Heat Stress-Induced Oxidative Stress and Skeletal Muscle Homeostasis Disruption by Promoting Autophagy in Mice DOI Open Access
Jiayin Lu,

Chao-yue Li,

Tong Zhao

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(4), P. 696 - 696

Published: Feb. 15, 2025

The gradual rise in global temperatures can affect skeletal muscle development and intestinal microorganisms. However, the influence of microbial metabolites on homeostasis under heat stress (HS) remains unclear. C57BL/6J mice were exposed to normal temperature or 40 °C conditions for 3 d, 7 14 d. HS d also treated with sodium butyrate (NaB, 200 mg/kg, gavage). Strikingly, body weight, antioxidative ability (MDA, T-SOD, GSH-Px), average cross-sectional area decreased, but blood glucose core increased HS. NaB treatment reversed these effects. Meanwhile, levels TNF-α CORT. Additionally, led a reduction villus height an increase crypt depth intestine. Microbial 16S rRNA sequencing analysis revealed that caused gut microbiota dysbiosis. expression HSP70 HS, maintain homeostasis. stimulated Pax7, which indicates was disrupted. expressions MyoG MyoD decreased immunofluorescence results show triggered shift from slow fibers (MYH7) fast (MYH1). recovered muscle-related factors. inhibited autophagy initiation (mTOR, Beclin1, Atg5, Atg7, Atg12), formation (LC3 II/LC3 I) autophagosomes, binding (p62 LAMP1) lysosomes activated by NaB. C2C12 cells H2O2 simulate oxidative stress, advance. Oxidative disrupted cells, characterized Pax decreases MyoD, changes treatment. unable stable Pax7 when inhibited. This suggests regulate induced through

Language: Английский

Citations

0
Oleanolic Acid Alleviates Hyperuricemia via Gut Microbiota Control the Integrity of Gut Barrier and the Expressions of Urate Transporter in Mice DOI

Tingting Zhang,

Siyan Liu,

Shenlin Liu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Hyperuricemia (HUA) is a globally prevalent metabolic disorder characterized by an imbalance in uric acid (UA) production and excretion. In this study, we found that oleanolic (OA), natural pentacyclic triterpene, effectively reduced HUA associated kidney injury C57BL/6J mice. A 12-week OA treatment significantly dose-dependently UA creatinine levels serum urine while suppressing hepatic xanthine oxidase activity Mechanistic analysis revealed modulates the expression of urate transporters including ABCG2, GLUT9, URAT1 small intestine. Furthermore, restored gut microbiota imbalances, increased short-chain fatty production, enhanced expressions intestinal tight junction proteins mice, thereby improving barrier integrity Consequently, fecal transplantation (FMT) was employed to illustrate major mediating role OA's alleviation Recipient mice transplanted with feces from OA-treated exhibited lower blood urinary levels, inflammation, improved balance compared those receiving untreated (p < 0.05). Additionally, FMT normalized transporter reinforced junctions recipient These findings underscore mitigates primarily modulating microbiota, regulating expression, reinforcing integrity, offering novel insights into its preventive potential for managing related complications.

Language: Английский

Citations

0
Red snapper protein prevents chronic unpredictable mild stress-induced ileal barrier damage by reversing microbiota-derived butyric acid overload DOI
Chen Wang,

Jinjin Luo,

Shuo Wang

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106233 - 106233

Published: Feb. 1, 2025

Language: Английский

Citations

0
The human gut microbiota and uric acid metabolism: genes, metabolites, and diet DOI
Lei Sun, Min Zhang,

Jianxin Zhao

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 21

Published: March 11, 2025

Hyperuricemia (HUA), characterized by an excessive production of uric acid (UA), poses a significant risk for various metabolic disorders and affects over one billion individuals globally. The intricate interplay between the gut microbiota dietary constituents plays pivotal role in maintaining UA homeostasis. Abnormal consumption specific components such as purines, fructose, or aberrant expression urate transporters can disrupt balance, precipitating HUA gout. exerts profound influence human regulation, particularly presence gene clusters. Individuals with often exhibit dysbiosis, reduction bacteria producing short-chain fatty acids those capable degrading UA, alongside increase opportunistic pathogens. Dietary their microbial metabolites engage interactions to modulate HUA, regulating inflammatory responses, suppressing xanthine oxidase activity curtail production, enhancing excretion via transporters. This comprehensive review delineates factors metabolism elucidating underlying mechanisms regulation. By unraveling connections metabolism, it offers valuable guidance grappling HUA.

Language: Английский

Citations

0