Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage DOI Creative Commons
Hatasu Kobayashi,

Yuichiro Hirao,

Shosuke Kawanishi

et al.

Genes and Environment, Journal Year: 2024, Volume and Issue: 46(1)

Published: June 3, 2024

Abstract Background Rosmarinic acid (RA) has a wide range of beneficial effects on human health. On the other hand, RA been reported to induce metal-mediated reactive oxygen species (ROS) generation and DNA damage. However, its mechanism remains unknown. In this study, clarify underlying mechanism, we analyzed damage in isolated treated with analog isorinic acid. Results plus Cu(II), but not Fe(III), significantly increased 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxodG) formation, an indicator oxidative damage, calf thymus DNA. Furthermore, comparison 8-oxodG formation induced by suggested that catechol groups could be associated their abilities form 8-oxodG. Interestingly, Cu(II) was markedly enhanced addition NADH, endogenous reductant. To elucidate Cu(II)-induced examined 32 P-labeled presence Cu(II). caused cleavage, which piperidine treatment, suggesting causes only strand breakage also base modification. inhibited catalase (H 2 O scavenger), bathocuproine (Cu(I) chelator), methional (scavenger variety ROS than • OH) typical OH scavengers SOD, indicating involvement H , Cu(I), OH. cleavage site analysis showing RA-induced site-specific (frequently at thymine some cytosine residues) supports OH, because without specificity. Based these results, Cu(I) concomitant autoxidation lead production Cu(I)-hydroperoxide, induces o -Quinone -semiquinone radicals are likely again reduced dramatically increases particularly low concentrations RA. Conclusions physiologically relevant effectively through redox cycle reactions copper NADH.

Language: Английский

Characterization of chemical compounds of fractions from four different bee pollens with chemometric classification DOI Creative Commons
Gülsen Tel‐Çayan, Fatih Çayan, Ebru Deveci

et al.

European Food Research and Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Abstract Bee pollen continues to increase its popularity as a ‘complete food’ among natural resources with high health value and therapeutic chemical compounds. This study focused on HPLC-DAD (high-performance liquid chromatography-diode array detection) based the identification of compounds Cistus creticus , Helianthus annuus Papaver somniferum Salix sp. bee pollens. Also, water extracts fractions (hexane, dichloromethane, ethyl acetate, butanol, water) obtained from methanol all pollens were investigated for A total 29 screened kaempferol was detected in studied samples at concentration 0.4 331.6 µg/g. Luteolin (68.7–694.8 µg/g), trans -aconitic acid (12.2–479.1 myricetin (160.4–1534.2 quercetin (162.1–608.1 rosmarinic (273.8–435.6 rutin (3.6–202.8 -cinnamic (5.1–1854.6 µg/g) found most abundant Additionally, nonpolar polar chemometrically clustered similarities differences via principal component analysis (PCA) hierarchical cluster (HCA). In fractions, C. H. P. acetate (CEA, HEA, PEA, SEA) separated other while butanol (CB, HB, PB, SB) fractions. provides additional data characterization valuable constitutes beginning chemometric classification

Language: Английский

Citations

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Quantification of amyloid-β aggregation inhibitors gallic acid and rosmarinic acid in biological samples by LC-MS/MS DOI Creative Commons
Raluca Ştefănescu

Analytical Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 115799 - 115799

Published: Feb. 1, 2025

The decline of the cognitive functions encountered at patients diagnosed with Alzheimer's disease (AD) together findings extracellular amyloid deposits, intracellular neurofibrillary tangles and microvascular angiopathy in brain were described beginning 20th century. According to cascade hypothesis, overproduction amyloid-β peptide its aggregation into neurotoxic oligomers, fibrils, plaques is considered cause AD. Amyloid-β fibril formation was experimentally proven vitro using thioflavin T assay absence interfering chemical compounds became an analytical tool for assessing effects different molecules against aggregation. Recent research studies provided experimental results that indicated reduction by gallic acid rosmarinic acid. Mass spectrometry often employed aiming identifying, characterizing, quantitating able modify progress purpose this review present current regarding identification quantitation water-soluble biological samples liquid chromatographs coupled triple quadrupole mass spectrometers as bioanalytical tools. study highlights presence amount these commonly used medicinal plants culinary herbs provides a list extraction chromatography electrospray-triple methods examples previous pharmacokinetic studies. article underlines bioavailability safety further preventing slowing

Language: Английский

Citations

0
Micropropagation, callus induction and cell culture establishment of Zataria multiflora (Lamiaceae): An efficient biotechnological platform for the production of rosmarinic acid DOI Creative Commons
Ziba Bakhtiar,

Foroogh Amoozad Khalili,

Masoud Ghasemi

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120701 - 120701

Published: Feb. 17, 2025

Language: Английский

Citations

0
Ehretia genus: a comprehensive review of its botany, ethnomedicinal values, phytochemistry, pharmacology, toxicology and clinical studies DOI Creative Commons
Sai Jiang, Mengyun Wang, Amanpreet Kaur

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 21, 2025

Background The Ehretia genus, comprising 66 species in the Boraginaceae family, has a history of ethnomedicinal use for various ailments. This review focuses on botany, traditional uses, phytochemistry, pharmacology, toxicology, clinical studies, cultivation, and commercial potential with goal enhancing current research applications. Methods Literatures related to were compiled using keywords such as “ ,” “traditional use,” “chemical constituents,” “bioactivity” from scientific databases, including “China Knowledge Resource Integrated Databases (CNKI),” “Flora China,” “Google Scholar,” “Hunan Library,” “Plants World Online,” “Web Science” so on. Results From 1980 August 2024, only 101 compounds have been identified within this primarily consisting flavonoids, phenylpropanoids, phenolics, benzoquinones, triterpenoids, fatty acids, phenylpropanoids being main components. Extracts exhibited bioactivities, antioxidant, hepatoprotective, analgesic, anti-inflammatory, antibacterial, anticancer effects, etc. Conclusion Research genus is limited, many remaining underexamined terms phytochemistry pharmacology. Few active isolated assessed biological activities, there lack investigation into their mechanisms action. Despite its documented remains less explored scientifically than other genera, presenting significant opportunities. Further comprehensive studies are necessary deepen our understanding diverse validate therapeutic potential.

Language: Английский

Citations

0
Bioactive Phytochemicals from Garlic (Allium sativum L.) By-Products DOI

Farhat Gul,

Zakir Ullah, Javed Iqbal

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Therapeutic drug delivery system for ischemic heart disease based on hydrogel carriers DOI
Yufeng He, Jun Tang,

Chengxi Wu

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106782 - 106782

Published: March 1, 2025

Language: Английский

Citations

0
Unraveling rosmarinic acid anticancer mechanisms in oral cancer malignant transformation DOI Creative Commons
Cátia Domingues, Ivana Jarak, Ana Miguel Matos

et al.

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 177466 - 177466

Published: March 1, 2025

Oral squamous cell carcinoma (OSCC) is expected to rise ca. 40 % by 2040. Rosmarinic acid (RA) has been recognized for its anticancer properties, although role in OSCC neglected. This work exploits the activity of RA 2D and 3D models cells compel a roadmap properties. The results demonstrated that significantly reduced mass metabolic dose, time, cell-type-dependent manner, predominantly highly-invasive OSCC, without compromising normal mucosa therapeutic doses. decreased mitochondria membrane potential increased redox state, which was corroborated pioneering observations on metabolome landscape (glutathione reduction acetate fumarate release). triggered autophagy, upregulating BNIP3 BCNL1 downregulating BIRC5. upregulation CADM1 downregulation VIM, CADM2, SNAIL1, SOX9 highlighted modulation epithelial-mesenchymal transition remodeling extracellular matrix MMP-2 MMP-9. interacts with P-glycoprotein highest docking score -6.4 Kcal/mol. surface charge after treatment (-22.6±0.3mV vs. -26.3± 0.3mV, p<0.0001), suggesting reversion polarity impairment invasion. also shrank growth multicellular tumor spheroids. Its modest protein binding human saliva sheds light administration oromucosal route. Overall, this supports need further research either monotherapy, combined conventional treatments, or conveyed nanosystems.

Language: Английский

Citations

0
Current Challenges and Recent Advancements in the Adoption of Omics to Enhance Biofortification DOI

Zakir Ullah,

Javed Iqbal, Banzeer Ahsan Abbasi

et al.

Published: Feb. 27, 2025

Language: Английский

Citations

0
Applications of Nanoparticles in Biofortification of Crops: Amplifying Nutritional Quality DOI

Zakir Ullah,

Javed Iqbal, Banzeer Ahsan Abbasi

et al.

Published: Feb. 27, 2025

Language: Английский

Citations

0
Anxiolytic, Antidepressant and Healthy Sleep-Promoting Potential of Rosmarinic Acid: Mechanisms and Molecular Targets DOI Creative Commons

Vijayan Priya,

Dilip Srinivasan,

Swagatika Priyadarsini

et al.

Neuropsychiatric Disease and Treatment, Journal Year: 2025, Volume and Issue: Volume 21, P. 641 - 661

Published: March 1, 2025

The etiology of psychiatric disorders is complex and results from intricate interactions among multiple neurobiological, psychological, environmental, genetic factors. Furthermore, the roles gut microbiome dyshomeostasis in their pathogeneses are just beginning to be uncovered, adding another level complexity. In recent years, significant efforts have been directed toward discovering multimodal yet safe therapeutics counteract psychological deficits. Rosmarinic acid (RA), a polyphenol found several medicinal herbs, has received considerable attention as potential multifaceted therapeutic agent, particularly for neuropsychiatric conditions. order critically evaluate this aspect, data was compiled consolidated after extensive searches on scholarly databases like PubMed, Google Scholar, Web Science. Peer-reviewed publications which focused RA agent were included regardless year publication country origin. Based pre-clinical clinical evidence, review delves into various mechanistic aspects antidepressant, anxiolytic, sleep-promoting functions RA. beneficial effects gut-microbiome-brain (GMB) axis implications regulation neuroprotective pathways also discussed, with particular focus exploiting them ameliorate Our assessment indicated that neuroprotectant against conditions beneficially influences plethora targets related redox, inflammatory, synaptic, cell death, neurotrophic, signaling pathways. As dietary may relevant favorably altering GMB axis, indicating its prospects adjuvant regulating pathogenic mechanisms underlying However, more studies required ascertain neuromodulatory actions neuronal pathophysiologies, including ailments.

Language: Английский

Citations

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