Bioactive Evaluation of Naringenin in Ameliorating Hyperuricemia‐Induced Liver Injury by Inhibiting Xanthine Oxidase

eFood, Journal Year: 2025, Volume and Issue: 6(1)

Published: Jan. 3, 2025

ABSTRACT Hyperuricemia (HUA) is one of main risk factors for liver injury, and xanthine oxidase (XOD) an important target HUA‐induced injury. As a typical natural active ingredient, naringenin (NAR) has been confirmed the good therapeutic effect on variety diseases. However, studies NAR ameliorating injury have not reported. Therefore, we evaluated bioactivity in investigated related molecular mechanisms. The inhibitory activity type XOD was by enzymatic reactions kinetic analyses, docking showed that able to bind tightly XOD. In vivo ameliorated function while being inhibit activity. alleviated oxidative stress caused excess reactive oxygen species through antioxidant At same time, exerted anti‐inflammatory regulating levels inflammatory factors. results suggested interact with Keap1 AMPK exhibit effects. This work demonstrated which valuable further development functional foods.

Language: Английский

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Mitigating Diabetic Cardiomyopathy: The Synergistic Potential of Sea Buckthorn and Metformin Explored via Bioinformatics and Chemoinformatics

Biology, Journal Year: 2025, Volume and Issue: 14(4), P. 361 - 361

Published: March 31, 2025

Diabetic cardiomyopathy (DCM), a critical complication of type 2 diabetes mellitus (T2DM), is marked by metabolic dysfunction, oxidative stress, and chronic inflammation, ultimately progressing to heart failure. This study investigated the synergistic therapeutic potential Hippophae rhamnoides L. (sea buckthorn, SBU) extract metformin in mouse model T2DM-induced DCM. T2DM was induced using 45% high-fat-AGEs-enriched diet, followed treatment with SBU, metformin, or their combination. Treatment effects were monitored through bioinformatic analysis, chemoinformatic prediction, behavioral testing, biochemical assays, histopathological evaluations gene expression profiles. Based on we identified key hub genes involved diabetic including SERPINE1, NRG1, MYH11, PTH, NR4A2, NRF2, PGC1α, GPX4, ATF1, ASCL2, NOX1, NLRP3, CCK8, COX2, CCL2, PTGS2, EGFR, oncostatin, which are pivotal modulating ferroptosis pathway. Furthermore, long non-coding RNAs (lncRNAs) NEAT1 MALAT1, regulators inflammation cell death, effectively downregulated, correlating decreased levels pro-inflammatory marker oncostatin. The combined therapy significantly improved glucose regulation, reduced systemic protected from damage. Histopathological analysis revealed notable reductions cardiac necrosis fibrosis. Particularly, combination SBU demonstrated effect, surpassing benefits individual treatments preventing These findings highlight integrating as novel strategy for managing DCM targeting both ferroptosis-related pathways. dual intervention opens promising avenues future clinical applications disease management, offering comprehensive approach mitigating progression

Language: Английский

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Hesperidin, vanillic acid, and sinapic acid attenuate atorvastatin‐induced mitochondrial dysfunction via inhibition of mitochondrial swelling and maintenance of mitochondrial function in pancreas isolated mitochondria

Drug Development Research, Journal Year: 2024, Volume and Issue: 85(4)

Published: May 30, 2024

Abstract It has been reported that lipophilic statins such as atorvastatin can more readily penetrate into β‐cells and reach the mitochondria, resulting in mitochondrial dysfunction, oxidative stress, decrease insulin release. Many studies have shown natural products protect dysfunction induced by drug different tissue. We aimed to explore protection potency of hesperidin, vanillic acid, sinapic acid compounds against pancreas isolated mitochondria. Mitochondria were form rat directly treated with toxic concentration (500 µM) presence various concentrations (1, 10, 100 separately. Mitochondrial toxicity parameters reactive oxygen species (ROS) formation, succinate dehydrogenases (SDH) activity, swelling, depletion glutathione (GSH), membrane potential (MMP) collapse, malondialdehyde (MDA) production measured. Our findings demonstrated at 500 μM higher pancreatic Except MDA, caused significantly reduction SDH ROS GSH, collapse MMP. While, our data showed all three protective low ameliorated atorvastatin‐induced increase improvement MMP formation. conclude reverse which may be beneficial for diabetogenic‐induced β‐cells.

Language: Английский

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Enhancing the therapeutic effect of infliximab by inhibiting ferroptosis of M2 macrophages in experimental colitis

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 10, 2024

Objective Drug combination presents a promising approach to surpassing the current efficacy limitations of biological agents in treating inflammatory bowel disease (IBD). Currently, ferroptosis has emerged as novel therapeutic target for IBD. Therefore, combining inhibitors with biologics may provide new strategy break ceiling IBD treatment. Thus, this study investigated whether could enhance infliximab (IFX) on Methods Immunofluorescence was used analyze M2 macrophages human colon specimens pre- and post-IFX The effect IFX M1 assessed RAW264.7 in vitro. Moreover, DSS-induced colitis mouse model employed evaluate impact vivo. Results Although were increased patients who responded treatment, there no concurrent increase non-responders which suggested that closely related macrophage. Notably, enhanced vitro, while more sensitive than macrophages. Finally, inhibitor deferoxamine by significantly alleviating mucosa experimental mice also had protective undergoing IFX. Conclusions results rescuing from ferroptosis, thereby offering overcoming biologic therapy.

Language: Английский

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The role of ALDHs in lipid peroxidation-related diseases

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138760 - 138760

Published: Dec. 1, 2024

Language: Английский

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