Chinese Medicine in Colorectal Cancer Treatment: From Potential Targets and Mechanisms to Clinical Application

Chinese Journal of Integrative Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Language: Английский

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Berberine promotes lacteal junction zippering and ameliorates diet-induced obesity through the RhoA/ROCK signaling pathway

Phytomedicine, Journal Year: 2023, Volume and Issue: 124, P. 155268 - 155268

Published: Dec. 7, 2023

Obesity has emerged as a global epidemic. Recent research indicated that diet-induced obesity can be prevented by promoting lacteal junction zippering. Berberine, which is derived from natural plants, found to promising in weight reduction, but the underlying mechanism remains unspecified. To determine whether berberine protects against regulating and explore potential molecular mechanisms. Following induction of obese (DIO) model, mice were administered low high doses for 4 weeks. Indicators associated with insulin resistance lipid metabolism examined. Various methods, such Oil Red O staining, transmission electron microscopy imaging, confocal imaging others used observe effects on absorption junction. In vitro, human dermal lymphatic endothelial cells (HDLECs) investigate effect LEC junctions. Western Blot immunostaining applied expression levels relevant molecules. Both reduced body DIO without appetite suppression ameliorated glucolipid disorders. We also loss might contribute inhibition small intestinal absorption. The possible was related promotion zippering via suppressing ras homolog gene family member A (RhoA)/Rho-associated kinase (ROCK) signaling pathway. promoted formation stable mature junctions HDLECs, involving same Berberine could promote ameliorate through RhoA/ROCK

Language: Английский

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Berberine attenuates TNBS-induced colitis in mice by improving the intestinal microbiota

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 29, 2024

To investigate the effects of berberine (BBR) as a treatment on intestinal microecological alterations and enteritis in mice produced by TNBS. There were seven per group: healthy group (Ctrl), TNBS-induced (TNBS), (BBR). The weighed, slaughtered after 7 days, subjected to high-throughput analysis Illumina, well haematological detection imaging evaluation colon pathology. length, immune cell subpopulations, inflammatory factors, microecology induced BBR refined using battery experiments observations. According studies, can increase number bacteria, including Lactobacillus, Verrucomicrobia, Bacteroides, Akkermansia muciniphila. has therapeutic effect colitis mice, which is associated with modifications subpopulations microecology. It also offers viable approach prospective probiotic (like muciniphila) IBD therapy clinical settings.

Language: Английский

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Quality markers of Guchang Zhixie pills based on multicomponent qualitative and quantitative analysis combined with network pharmacology and chemometric analysis

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2023, Volume and Issue: 240, P. 115934 - 115934

Published: Dec. 25, 2023

Language: Английский

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Definition of the Neurotoxicity-Associated Metabolic Signature Triggered by Berberine and Other Respiratory Chain Inhibitors

Antioxidants, Journal Year: 2023, Volume and Issue: 13(1), P. 49 - 49

Published: Dec. 28, 2023

To characterize the hits from a phenotypic neurotoxicity screen, we obtained transcriptomics data for valinomycin, diethylstilbestrol, colchicine, rotenone, 1-methyl-4-phenylpyridinium (MPP), carbaryl and berberine (Ber). For all compounds, concentration triggering neurite degeneration correlated with onset of gene expression changes. The mechanistically diverse toxicants caused similar patterns regulation: responses were dominated by cell de-differentiation canonical stress response pathways driven ATF4 NRF2. obtain more detailed specific information on modes-of-action, effects energy metabolism (respiration glycolysis) measured. Ber, rotenone MPP inhibited mitochondrial respiratory chain they shared complex I as target. This group was further evaluated metabolomics under experimental conditions that did not deplete ATP. Ber (204 changed metabolites) showed rotenone. overall metabolic situation characterized oxidative stress, an over-abundance NADH (>1000% increase) re-routing in order to dispose nitrogen resulting increased amino acid turnover. unique pattern led accumulation metabolites known biomarkers neurodegeneration (saccharopine, aminoadipate branched-chain ketoacids). These findings suggest inhibitors may result ensemble changes rather than simple ATP depletion. combi-omics approach used here provided richer MoA common analysis alone. As human drug food supplement, mimicked closely mode-of-action neurotoxicants, its potential hazard requires investigation.

Language: Английский

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Traditional Chinese Medicine formula Dai-Zong-Fang alleviating hepatic steatosis in db/db mice via gut microbiota modulation

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 24, 2024

Introduction: Hepatic steatosis is a hepatic pathological change closely associated with metabolic disorders, commonly observed in various diseases such as syndrome (MetS), high global prevalence. Dai-Zong-Fang (DZF), traditional Chinese herbal formula, widely used clinical treatment for MetS, exhibiting multifaceted effects reducing obesity and regulating blood glucose lipids. This study aims to explore the mechanism by which DZF modulates gut microbiota reduces based on gut-liver axis. Methods: utilized db/db mice disease model drug intervention. Body weight fasting were monitored. Serum lipid transaminase levels measured. Insulin tolerance test was conducted assess insulin sensitivity. Hematoxylin eosin (HE) staining employed observe morphological changes liver intestine. The degree of evaluated through Oil Red O determination. Changes assessed using 16S rRNA gene sequencing. lipopolysaccharide (LPS) measured ELISA. expression intestinal tight junction proteins, absorption-related key proteins metabolism examined Western blot RT-qPCR. Results: After intervention, there decrease body weight, alleviation reduction serum aspartate aminotransferase (AST) alanine (ALT) levels, mitigation resistance mice. significantly modulated diversity microbiota, notable increase abundance Bacteroidetes phylum. PICRUSt indicated that influenced functions including carbohydrate amino acid metabolism. Following LPS decreased, damage reduced, protein occludin increased, while cluster differentiation 36 (CD36) apolipoprotein B48 (ApoB48) decreased. In liver, intervention resulted droplets, accompanied fatty synthase (FASN) stearoyl-CoA desaturase 1 (SCD1) transport 2 (FATP2). Conversely, an oxidation-related enzyme carnitine palmitoyltransferase-1𝛂 (CPT-1𝛂). Conclusion: can regulate structure function ameliorates barrier detrimental endotoxemia not only inhibits absorption but also improves from aspects, de novo lipogenesis, uptake, oxidation. suggests may act intestine target organs, exerting its improving related functions, ultimately ameliorating enhancing overall

Language: Английский

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Berberine inhibits colorectal liver metastasis via modulation of TGF-β in a cecum transplant mouse model

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: Nov. 19, 2024

Language: Английский

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Berberine and Cyperus rotundus extract nanoformulations protect the rats against Staphylococcus-induced mastitis via antioxidant and anti-inflammatory activities: role of MAPK signaling

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 21, 2024

Language: Английский

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