Silver Nanoparticles with Modified Synthesis Use Sodium Borohydride Reducing Agent and Okra (Abelmoschus Esculentus L.) Raw Polysaccharide Extract as an Anti-Colon Cancer

Trends in Sciences, Journal Year: 2024, Volume and Issue: 21

Published: July 24, 2024

Nanomedicine using nanoparticles has become a new trend in the treatment of colon cancer. Nanosilver is known to have antibacterial, antifungal, antidiabetic, and anticancer properties. The most common easy way make nanosilver reducing agent sodium borohydride, but this inorganic compound element high toxicity body. Polysaccharides long been as agents with low few side effects. Okra (Abelmoschus esculentus L.), flowering plant from Malvaceae family found tropical subtropical areas, crude polysaccharide extract pods highest content. This research aims determine effect raw okra (ORPE) for making on its ability an anti-colon cancer cell line HCT 116. Experiments were carried out by modifying optimizing manufacture AgNPs NaBH4 ORPE, 16 concentrations each repetition 3 times. Characteristic tests UV-Vis Spectrophotometry, Particle Size Analyzer, Scanning Electron Microscopy, Transmission viability testing MTT assay, evaluation potential apoptosis necrosis Annexin V-PI flow cytometry. PSA test average particle size. Two groups 1 (AgNP-NaBH4) 2 (AgNP-ORPE) had repeated measurements times concentrations. mean zeta value group = 232.5 ± 25.47 nm / –42.23 1.45 mV 779.66 112.45 –23.15 3.65 mV. TEM showed that size Group was 50.85 (ꭓ 113.14 nm) 121.43 248.52 nm). SEM morphology both round shape (group slight agglomeration). absorbance spectrum formed at wavelength 389 1) 281.5 2). IC50 obtained 76.68 mmol/L 60.3 % apoptotic cells, 3.74 92.58 81.4 cells 4.95 necrosis. ORPE nanosilver-reducing proven induce death cause changes mitochondrial membrane permeability intrinsic pathway apoptosis.

Language: Английский

Redefining the role of autophagy in diabetic vascular diseases

Exploration of Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

Diabetic vascular disease, as one of the common complications in diabetic patients, threatens quality life and health patients. Autophagy maintains cellular homeostasis survival an important intracellular self-repair mechanism. In recent years, with gradual deepening autophagy research, more studies have found that cells such endothelial cells, smooth muscle inflammatory are closely associated various disorders. Different regulatory mechanisms can lead to different or similar outcomes, there complex crosstalk process. Therefore, we will summarize latest research progresses regards role focusing on mitophagy, epigenetic modifications, apoptosis, inflammation, development aiming provide effective therapeutic targets for injury.

Language: Английский

Augmenter of liver regeneration inhibits renal fibrosis during acute kidney injury to chronic kidney disease transition by regulating autophagic flux

Archives of Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 764, P. 110218 - 110218

Published: Nov. 27, 2024

Language: Английский