Innovative Approach: MRI-Guided Fabrication of a Biomimetic Intervertebral Disc Scaffold
Abstract
Study
Design ：A
biomimetic
intervertebral
disc
(IVD)
scaffold
was
fabricated
through
the
integration
of
magnetic
resonance
imaging
(MRI)
and
3D
printing
technology,
subsequently
underwent
in
vitro
experimentation.
Objective： This
study
aimed
to
create
a
new
IVD
using
MRI
grayscale
image
analysis
GE-DLP
technology
mimic
natural
structure
for
improved
biomechanical
performance
cell
compatibility,
potentially
providing
treatment
option
regeneration.
Summary
Background
Data:
The
is
critical
component
spine,
its
damage
or
degeneration
can
lead
severe
back
neck
pain.
Current
treatments
provide
symptomatic
relief
but
do
not
address
structural
damage.
Tissue
engineering
offers
promising
alternative,
with
bio-scaffolds
being
key
element
Methods:
In
this
study,
we
constructed
model
from
scans
healthy
volunteer
processed
images
distinguish
between
tissue
types.
Exposure
times
were
adjusted
based
on
values,
applied
fabricate
single
integrated
process
bicomponent
polymer
network
(BCN)
hydrogel
laden
nucleus
pulposus
stem
cells
(NPMSCs).
The
microstructure
porosity
analyzed
scanning
electron
microscopy
(SEM),
elastic
modulus
across
radial
distribution
tested
via
nanoindentation.
evaluated
finite
(FEA).
For
biocompatibility
assessment,
cytoskeleton
staining
utilized
observe
morphology,
viability
Calcein/PI
staining.
Results:
exhibited
gradient
changes
pore
size,
consistent
ultrastructure
characteristics
IVD.
FEA
indicated
that
scaffold's
response
terms
displacement,
strain,
stress
closely
resembled
an
actual
IVD,
particularly
during
simulations
left
rotation
lateral
bending.
SEM
revealed
pores
varying
sizes
different
regions
scaffold,
which
crucial
adhesion
growth.
also
demonstrated
high
biocompatibility,
survival
rates
maintained
at
level
over
seven-day
culture
period.
Conclusion:
research,
have
successfully
engineered
novel
excellent
static
integrity
by
integrating
technology.
Level
Evidence:
N/A
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 21, 2024
Language: Английский