Lipid Nanoparticle-Mediated Oip5-as1 Delivery Preserves Mitochondrial Function in Myocardial Ischemia/Reperfusion Injury by Inhibiting the p53 Pathway

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(45), P. 61565 - 61582

Published: Nov. 1, 2024

Myocardial ischemia/reperfusion (MI/R) injury, a major contributor to poor prognosis in patients with acute myocardial infarction, currently lacks effective therapeutic strategies clinical practice. The long noncoding RNA (lncRNA) Oip5-as1 can regulate various cellular processes, such as cell proliferation, differentiation, and survival. may have potential target for MI/R injury its upregulated expression has been associated reduced infarct size improved cardiac function animal models, although how effectively safely overexpress vivo remains unclear. Lipid nanoparticles (LNPs) are versatile technology targeted drug delivery numerous applications. Herein, we aimed assess the efficacy safety of LNPs coloaded cardiomyocyte-specific binding peptide (LNP@Oip5-as1@CMP) murine model injury. To achieve this, LNP@Oip5-as1@CMP was synthesized via ethanol injection method. structural components were physicochemically analyzed. A hypoxia/reoxygenation (H/R) HL-1 cells coronary artery ligation mice used simulate Our results demonstrated that designed cardiomyocyte targeting efficient successfully synthesized. In cells, treatment significantly mitochondrial apoptosis caused by H/R model, intravenous administration decreased function. Mechanistic investigations revealed inhibited p53 signaling pathway. However, cardioprotective effects abrogated administrating Nutlin-3a, activator. Furthermore, no signs organ damage detected after injection. study reveals mitigating These findings pave way advanced treatments cardiovascular diseases, emphasizing promise lncRNA-based therapies.

Language: Английский

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Non-coding RNAs in myocardial ischemia/reperfusion injury and repair

Current Opinion in Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 100825 - 100825

Published: March 1, 2025

Language: Английский

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Comprehensive evaluation of non-coding RNA-mediated autophagy regulation in myocardial ischemia-reperfusion injury

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 25, 2025

Ischemic heart disease remains a major global health challenge, with myocardial ischemia-reperfusion injury (MIRI) being one of its most common and severe pathophysiological complications. The pathogenesis MIRI is multifaceted, involving oxidative stress, inflammatory responses, apoptotic pathways, autophagic regulation. Notably, autophagy exerts dual regulatory effect, where maintaining optimal flux essential for cardiac homeostasis. Emerging evidence underscores the crucial role non-coding RNAs (ncRNAs) in modulating these pathological processes. In particular, long (lncRNAs), microRNAs (miRNAs), circular (circRNAs) have been identified as key regulators autophagy-mediated progression through complex molecular networks. This review provides systematic analysis pathways which ncRNAs influence pathogenesis, specific focus on their autophagy-regulatory mechanisms. These insights may enhance our understanding pathobiology facilitate development novel therapeutic strategies.

Language: Английский

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Oxidative Stress in Cardiovascular Disorders

Advances in medical diagnosis, treatment, and care (AMDTC) book series, Journal Year: 2025, Volume and Issue: unknown, P. 311 - 338

Published: Jan. 3, 2025

Cardiovascular disorders (CVDs) include atherosclerosis, cardiac failure, hypertension, myocardial infarction, and oxidative stress, which are increasingly recognized as essential factors for the onset advancement of these conditions. he imbalance between formation reactive oxygen species (ROS) antioxidant defense system is molecular basis leads to damage lipids, proteins, DNA. This potential promotes inflammatory responses, disrupts endothelial function, speeds up atherogenesis. The nitric oxide synthase uncoupling, mitochondrial dysfunction, NADPH-oxidizing enzymes important pathways that enhance ROS production. Antioxidants including polyphenols, vitamins E C, others have shown conflicting outcomes in clinical studies. These novel methods aim improve natural defenses, alter redox-sensitive signaling pathways, target specific sources.

Language: Английский

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Role of long non‐coding RNAs in cholangiocarcinoma: A systematic review and meta‐analysis

Cancer Reports, Journal Year: 2024, Volume and Issue: 7(3)

Published: March 1, 2024

Abstract Background Cholangiocarcinoma (CCA), as a rare malignancy of the biliary tree, has poor prognosis most time. CCA is highly epigenetically regulated and several long non‐coding RNAs (lncRNA) have been investigated to diagnostic prognostic role in CCA. The current study aimed assess studies finding relevant lncRNAs systematically. Methods International databases, including PubMed, Cochrane Library, Embase, were comprehensively searched order identify investigating any lncRNA After screening by title/abstract full‐text, necessary data extracted. Random‐effect meta‐analysis was performed for pooling areas under curve (AUCs), specificity, sensitivity diagnosis Results A total 33 chosen be included final analysis, comprised 2677 patients. Meta‐analysis AUCs evaluation resulted pooled AUC 0.79 (95% CI: 0.75–0.82; I 2 = 69.11, p < .01). Additionally, overall 0.80 CI 0.75–0.84) specificity 0.77 0.68–0.84) observed. Measurement lncRANs assessment also improved survival significantly (effect size 1.61, 95% 1.39–1.82). similar result found progression‐free 1.57, 1.20–1.93). Conclusion Based on our findings, showed promising results biomarkers since they had acceptable addition fact that this cancer. Further might needed address issue find best clinically useful lncRNA.

Language: Английский

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Recent Insights into Endogenous Mammalian Cardiac Regeneration Post-Myocardial Infarction

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11747 - 11747

Published: Nov. 1, 2024

Myocardial infarction (MI) is a critical global health issue and leading cause of heart failure. Indeed, while neonatal mammals can regenerate cardiac tissue mainly through cardiomyocyte proliferation, this ability lost shortly after birth, resulting in the adult heart's inability to injury effectively. In mammals, adverse remodelling, which compensates for loss cells, impairs function due non-contractile nature fibrotic tissue. Moreover, neovascularisation MI inadequate restore blood flow infarcted myocardium. This review aims synthesise most recent insights into molecular cellular players involved endogenous myocardial vascular regeneration, facilitating identification mechanisms that could be targeted trigger reduce fibrosis, improve functional recovery post-MI. Reprogramming cardiomyocytes regain their proliferative potential, along with modulation target cells responsible neovascularisation, represents promising therapeutic strategies. An updated overview regulate both coronary vasculature regeneration-including stem progenitor growth factors, cell cycle regulators, key signalling pathways-could help identify new intervention points applications.

Language: Английский

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MIR193BHG inhibits the proliferation, migration and invasion of trophoblasts by upregulating p53

Experimental and Therapeutic Medicine, Journal Year: 2024, Volume and Issue: 28(2)

Published: June 17, 2024

Aberrant expression of long non‑coding RNAs (lncRNAs) serves a crucial role in the biological function trophoblasts and contributes to preeclampsia (PE). lncRNA MIR193BHG is increased PE placental tissues. In present study, effects on were assessed elucidate its underlying molecular mechanisms. The subcellular localization HTR‑8/SVneo human first‑trimester extravillous trophoblast cells was determined using fluorescent in situ hybridization assay by conducting nucleocytoplasmic separation. effect knockdown or overexpression proliferation, migration, invasion apoptosis evaluated vitro Cell Counting Kit‑8, wound healing, Transwell flow cytometry assays. RNA‑sequencing, Kyoto Encyclopedia Genes Genomes pathway enrichment analysis protein‑protein interaction network construction subsequently performed screen downstream molecules regulated MIR193BHG. Finally, rescue experiments conducted ascertain whether influenced via p53. predominantly localized nucleus significantly inhibited migration invasion, while increasing rate cells. Knockdown had opposite effect. Furthermore, led increases both mRNA protein levels p53 compared with control group, rescued induced cell partially counteracting conclusion, findings study suggested that served critical progression regulating p53, may be novel therapeutic target for PE.

Language: Английский

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The Role of Long Non-Coding RNAs in Cardiovascular Diseases: A Comprehensive Review

Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 11, P. 158 - 187

Published: Dec. 28, 2024

Cardiovascular diseases (CVDs) are the leading cause of morbidity and mortality worldwide, posing significant challenges to healthcare systems. Despite advances in medical interventions, molecular mechanisms underlying CVDs not yet fully understood. For decades, protein-coding genes have been focus CVD research. However, recent genomics highlighted importance long non-coding RNAs (lncRNAs) cardiovascular health disease. Changes lncRNA expression specific tissues may result from various internal or external factors, tissue damage, organ dysfunction, In this review, we provide a comprehensive discussion regulatory lncRNAs their roles pathogenesis progression CVDs, such as coronary heart disease, atherosclerosis, failure, arrhythmias, cardiomyopathies, diabetic cardiomyopathy, explore potential therapeutic targets diagnostic biomarkers.

Language: Английский

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The importance of biomarkers of post-myocardial infarction heart failure: a systematic review

Deleted Journal, Journal Year: 2023, Volume and Issue: 2, P. 262 - 262

Published: Dec. 30, 2023

Heart failure (HF) after a myocardial infarction (MI) is one of the most frequent complications and with highest mortality rate in population. Given this, importance finding appropriate biomarkers for diagnosis, prognosis treatment this condition. The review was carried out under an analytical approach, using PRISMA statement as methodology; documents were retrieved through Scopus, ScienceDirect PubMed databases, where 20 articles that met certain inclusion criteria recognized. In conclusion, it explained use standard such B-type natriuretic peptide (BNP) non-terminal pro-BNP clinical practice have limitations, especially due to their low specificity HF post-MI complication. Therefore, currently, reflect alteration cardiac integrity, injury necrosis, inflammatory biomarkers, fibrosis, biomechanical stress, other types non-coding RNAs are being investigated

Language: Английский

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