Rethinking Fat Browning: Uncovering New Molecular Insights into the Synergistic Roles of Fasting, Exercise, and Cold Exposure

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 177651 - 177651

Published: April 1, 2025

Language: Английский

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Effects of Nicotinamide Riboside Supplementation on Postmortem Mitochondrial Functionality and Apoptotic Activation

Metabolites, Journal Year: 2025, Volume and Issue: 15(1), P. 31 - 31

Published: Jan. 8, 2025

Early postmortem mitochondrial function and apoptotic activation affect meat quality development. Nicotinamide riboside (NR) supplementation to pigs prior harvest can improve pork color stability, but its mechanism remains unclear. This study aimed evaluate the impact of NR on early functionality apoptosis. Sixteen (N = 16) were individually fed a control or NR-supplemented diet (30 mg·kg body weight-1·d-1) for 10 days harvest. Longissimus dorsi muscle samples collected at 45 min 24 h analyzed using high-resolution respirometry protein abundance (apoptosis regulator Bcl-2-associated X (BAX), inducing factor (AIF), caspase 3 (CASP3)) via immunoblotting. exhibited lower proton leak-associated respiration (p < 0.05), followed by slower accumulation outer membrane permeabilization (MOMP; p 0.05) loss integral from postmortem. decreased BAX increased mature AIF (62 kDa) 0.05). The CASP3 fragments (~29 postmortem, independent treatment demonstrated potential protect alleviate in porcine skeletal muscle, which might contribute higher stability during retail display.

Language: Английский

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Oroxylin A alleviates pyroptosis and apoptosis in human corneal epithelial cells under hyperosmotic stress by activating the SIRT3-SOD2/HIF-1α pathway

Experimental Eye Research, Journal Year: 2025, Volume and Issue: 255, P. 110345 - 110345

Published: March 15, 2025

Language: Английский

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A study on the molecular mechanism of cardiac protection of procarboxylpeptidase in MIRI rats based on the NLRP3 signaling pathway

Science Progress, Journal Year: 2025, Volume and Issue: 108(1)

Published: Jan. 1, 2025

Objective To investigate the way in which prolylcarboxypeptidase (PRCP) protects against myocardial ischemia-reperfusion injury (MIRI) and mechanisms that underlie it. Method A surgical ligation of coronary artery was adopted to establish a model male SD rats. Thirty-six rats were randomly divided into six groups: Normal group, Sham MIRI empty vector (MIRI + EZ.null) PRCP overexpression PRCP) nicorandil Nic) with 6 each group. The received an injection PRCP's adeno-associated virus 9 (AAV9) through tail vein 3 weeks prior modeling. Results Compared groups, expression levels NOD-like receptor thermal protein domain-associated (NLRP3), Caspase-1, IL-18, IL-1β, GSDMD group EZ-null significantly increased ( P < 0.05). Myocardial apoptosis index, infarction size, ejection fraction, short axis shortening rate At same time, therapy could reverse damage effect caused by Conclusion can lessen protect cardiac function inhibiting NLRP3/Capase-1/IL-18/IL-1β signaling pathway-mediated cell pyroptosis.

Language: Английский

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Cardioprotective effect of 19,20-epoxydocosapentaenoic acid (19,20-EDP) in ischaemic injury involves direct activation of mitochondrial sirtuin 3

Cardiovascular Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Although current clinical therapies following myocardial infarction have improved patient outcomes, morbidity, and mortality rates secondary to ischemic ischemia reperfusion (IR) injury remains high. Maintaining mitochondrial quality is essential limit damage cardiac IR injury. The deacetylase sirtuin 3 (SIRT3) plays a pivotal role in regulating function energy metabolism. In the study, we hypothesize that 19,20-epoxydocosapentaenoic acid (19,20-EDP) attenuates via stimulating SIRT3.

Language: Английский

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The Identification of Key Genes and Biological Pathways in Cardiac Arrest by Integrated Bioinformatics and Next Generation Sequencing Data Analysis

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

Abstract Cardiac arrest (CA) is a common cause of death world wide. The disease has lacks effective treatment. Efforts have been made to elucidate the molecular pathogenesis CA, but mechanisms remain elusive. To identify key genes and pathways in next generation sequencing (NGS) GSE200117 dataset was downloaded from Gene Expression Omnibus (GEO) database. DESeq2 tool used recognize differentially expressed (DEGs). ontology (GO) REACTOME pathway enrichment analyses were performed analyze DEGs associated signal g:Profiler IID database construct protein-protein interaction (PPI) network, modules analysis using Cytoscape. A miRNA-hub gene regulatory network TF-hub then constructed screen miRNAs, TFs hub by miRNet NetworkAnalyst Cityscape software. Receiver operating characteristic curve (ROC) verified genes. In total, 844 identified, comprising 414 up regulated 430 down GO indicated that for CA mainly enriched organonitrogen compound metabolic process, response stimulus, translation immune system. Ten (up-regulated: HSPA8, HOXA1, INCA1 TP53; down-regulated: HSPB1, LMNA, SNCA, ADAMTSL4 PDLIM7) screened. We also predicted miRNAs (hsa-mir-1914-5p hsa-mir-598-3p) (JUN PRRX2) targeting This study uses series bioinformatics technologies obtain hug genes, TFs, related CA. These results provide us with new ideas finding biomarkers treatment methods

Language: Английский

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Elucidating the Role of Sirtuin 3 in Mammalian Oocyte Aging

Cells, Journal Year: 2024, Volume and Issue: 13(18), P. 1592 - 1592

Published: Sept. 22, 2024

The field of reproductive biology has made significant progress in recent years, identifying specific molecular players that influence oocyte development and function. Among them, sirtuin 3 (SIRT3) attracted particular attention for its central role mediating mitochondrial function cellular stress responses oocytes. So far, studies have demonstrated the knockdown SIRT3 leads to a decrease blastocyst formation an increase oxidative within embryo, underscoring importance maintaining redox balance critical embryonic survival growth. Furthermore, literature reveals signaling pathways, such as SIRT3- Glycogen synthase kinase-3 beta (GSK3β) deacetylation pathway, crucial mitigating stress-related anomalies meiosis, particularly under conditions like maternal diabetes. Overall, emerging regulating highlights understanding intricate connections between metabolism, response overall health This knowledge could lead novel strategies support quality fertility, with far-reaching implications assisted technologies women's healthcare. commentary aims provide overview oocytes by synthesizing results from multitude studies. aim is elucidate development, maturation, aging identify areas where further research needed.

Language: Английский

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Metabolomic Insight into the DCD Kidney Grafts – Normothermic Ex Vivo Kidney Perfusion Compared With Hypothermic Machine Perfusion and Static Cold Storage

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

One of the fundamental problems current transplantation is organ shortage. To address this issue, Extended Criteria Donor grafts are utilized. However, requires better preservation strategies and reliable methods assessing graft function. The objective work was to evaluate changes in kidney metabolome intact organs subjected three methods: normothermic ex vivo perfusion (NEVKP), hypothermic machine (HMP) static cold storage (SCS). chemical biopsy used for minimally invasive sampling metabolites, followed by analysis on liquid chromatography coupled a high-resolution mass spectrometry (LC-HRMS) platform. results revealed metabolites altered ischemia oxidative stress donor’s kidneys, as well compounds characterizing occurring perfused under conditions storage. metabolomic differences were also recorded immediately after reperfusion few days surgery. It clearly shown that all associated with different biochemical alterations. pattern NEVKP indicated sustaining enables activation physiological anti-oxidative anti-inflammatory mechanisms tissue. some protective known exhibit Janus’sface, which might be an important finding future design treatment. A metabolomics profile HMP SCS kidneys confirmed despite fact hypothermia lowers energy demand cells, it restricts repair mechanisms. studies lie ground experiments toward assessment improved directly from utilizing non-destructive approach.The can serve versatile tool discovery investigations, but translated into diagnostic monitor specific biomarkers quality future.

Language: Английский

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Optimization of Extraction Process of Total Flavonoids from Loquat Leaves and Its Protective Effect on H9C2 Cells in Hypoxia Injury

Journal of Pharmacy And Bioallied Sciences, Journal Year: 2024, Volume and Issue: 16(4), P. 149 - 153

Published: Oct. 1, 2024

To study the reflux extraction process of total flavonoids from loquat leaves and to investigate its protective effect on H9C2 cells in hypoxia injury. An orthogonal test was utilized optimize parameters, Na2S2O4 used induce myocardial cell injury, MTT assay evaluate survival rate, CK, LDH, SOD, MDA were injury cells. The optimized condition for 10 times amount 70% ethanol 3 times, each time 2 h. extracted content TFL 37.12 mg/g under this condition, found increase decrease levels MDA, improve SOD levels. favored is stable feasible, have a good

Language: Английский

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