Traversing the epigenetic landscape: DNA methylation from retina to brain in development and disease DOI Creative Commons
Chunxiu Xu, Xuefei Fu, Huan Qin

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Nov. 29, 2024

DNA methylation plays a crucial role in development, aging, degeneration of various tissues and dedifferentiated cells. This review explores the multifaceted impact on retina brain during development pathological processes. First, we investigate retinal then focus diseases, detailing changes patterns diseases such as diabetic retinopathy (DR), age-related macular (AMD), glaucoma. Since is considered an extension brain, its unique structure allows it to exhibit similar immune response mechanisms brain. We further extend our exploration from examining associated Alzheimer's disease (AD) Huntington's (HD) better understand mechanistic links between explore possibility communication visual system central nervous (CNS) epigenetic perspective. Additionally, discuss neurodevelopmental including schizophrenia (SZ), autism spectrum disorder (ASD), intellectual disability (ID), how affects neuronal synaptic plasticity, cognitive function, providing insights into molecular underlying disorders.

Language: Английский

From bench to bedside: cutting-edge applications of base editing and prime editing in precision medicine DOI Creative Commons

Weihui Xu,

Shiyao Zhang, Huan Qin

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 20, 2024

Abstract CRISPR-based gene editing technology theoretically allows for precise manipulation of any genetic target within living cells, achieving the desired sequence modifications. This revolutionary advancement has fundamentally transformed field biomedicine, offering immense clinical potential treating and correcting disorders. In treatment most diseases, genome that avoids generation mixed byproducts is considered ideal approach. article reviews current progress base editors prime editors, elaborating on specific examples their applications in therapeutic field, highlights opportunities improvement. Furthermore, we discuss performance these technologies terms safety efficacy applications, analyze latest advancements directions could influence future development technologies. Our goal to outline relevance this rapidly evolving scientific preview a roadmap successful DNA therapies hereditary or idiopathic diseases.

Language: Английский

Citations

5
Mesenchymal stem cells and mesenchymal stem cell-derived exosomes: a promising strategy for treating retinal degenerative diseases DOI Creative Commons

Wenjing An,

Wenliang Zhang, Qi Jia

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: Feb. 21, 2025

Abstract Mesenchymal stem cells (MSCs) have emerged as a promising therapeutic strategy in regenerative medicine, demonstrating significant potential for clinical applications. Evidence suggests that MSCs not only exhibit multipotent differentiation but also exert critical effects retinal degenerative diseases via robust paracrine mechanisms. protect from damage by modulating inflammation, inhibiting apoptosis, alleviating oxidative stress, and suppressing cell death pathways. Furthermore, contribute to structural functional stability facilitating vascular remodeling donating mitochondria cells. Of particular interest, MSC-derived exosomes gained widespread attention compelling cell-free therapy. Owing their potent anti-inflammatory, anti-apoptotic, vascular-stabilizing properties, show promise the treatment of diseases.

Language: Английский

Citations

0
Infection of human induced pluripotent stem cells by an oncogenic herpesvirus DOI Creative Commons

Jiaojiao Fan,

Zi-Han Lin, Huiliang Zhang

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: March 13, 2025

Objective As one of the major human oncogenic viruses, Kaposi’s Sarcoma-associated Herpesvirus (KSHV) is closely related to several cancers such as sarcoma (KS) and primary effusion lymphoma (PEL). KSHV can infect a broad tropism cells in vitro vivo . Embryonic stem cell-like pluripotent be generated by simultaneous introduction factors, into somatic cells, yielding induced (iPS) cells. However, it remains unclear whether (hiPSCs) are permissive how this virus infection may affect cellular gene profile. Methods In current study, we examined hiPSCs were infection. The flow cytometry was used assess impacts on viability apoptosis. Illumina RNA-Sequencing determine profile changed KSHV-infected lytically Results We report that successfully establishes latent hiPSCs, which completely lytic reactivation release infectious virions. de novo arrests growth through inducing cell Transcriptomic analysis revealed significant changes global expression well Conclusion Our findings demonstrate powerful tool explore potential functions pathogenesis differentiated

Language: Английский

Citations

0
The Role of Non-coding RNAs in Diabetic Retinopathy: Mechanistic Insights and Therapeutic Potential DOI

Zhuan Zuo,

Ni Li, Qian Zhang

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Molecular Subtypes and Targeted Therapeutic Strategies in Small Cell Lung Cancer: Advances, Challenges, and Future Perspectives DOI Creative Commons
Daoyuan Huang, Jingchao Wang, Li Chen

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(8), P. 1731 - 1731

Published: April 12, 2025

Small cell lung cancer (SCLC) is a highly aggressive malignancy characterized by rapid progression, early metastasis, and high recurrence rates. Historically considered homogeneous disease, recent multi-omic studies have revealed distinct molecular subtypes driven lineage-defining transcription factors, including ASCL1, NEUROD1, POU2F3, YAP1, as well an inflamed subtype (SCLC-I). These exhibit unique therapeutic vulnerabilities, thereby paving the way for precision medicine targeted therapies. Despite advances in classification, tumor heterogeneity, plasticity, therapy resistance continue to hinder clinical success treating SCLC patients. To this end, novel strategies are being explored, BCL2 inhibitors, DLL3-targeting agents, Aurora kinase PARP epigenetic modulators. Additionally, immune checkpoint inhibitors (ICIs) show promise, particularly immune-enriched of Hence, deeper understanding characteristics, evolution, regulatory mechanisms subtype-specific factors crucial rationally optimizing therapy. This knowledge not only facilitates identification targets, but also provides foundation overcoming developing personalized combination treatment strategies. In future, integration data, dynamic monitoring, approaches expected further advance translation therapies, ultimately improving patient survival outcomes.

Language: Английский

Citations

0
Anesthetic effects on electrophysiological responses across the visual pathway DOI Creative Commons
Shiyao Zhang,

Weihui Xu,

Shanshan Liu

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 13, 2024

Anesthetics are widely used in electrophysiological tests to assess retinal and visual system functions avoid experimental errors caused by movement stress animals. To determine the most suitable anesthetic for tests, excluding ketamine chloral hydrate due regulatory side effect concerns, this study investigated effects of ethyl carbamate (EC), avertin (AR), pentobarbital sodium (PS) on signal conduction retina primary cortex. Assessments included flash electroretinogram (FERG), pattern (PERG), evoked potentials (PVEP), (FVEP), FERG FVEP were evaluate responses cortex stimuli, respectively, while PERG PVEP assessed stimuli. The research showed that AR demonstrates least disruption pathway, as evidenced consistently high characteristic peaks group across various tests. In contrast, mice given EC exhibited lowest peak values both FVEP, subjects anesthetized with PS suppressed oscillatory responses. Notably, substantial observed only AR. Consequently, among three anesthetics tested, is studies.

Language: Английский

Citations

1
Traversing the epigenetic landscape: DNA methylation from retina to brain in development and disease DOI Creative Commons
Chunxiu Xu, Xuefei Fu, Huan Qin

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Nov. 29, 2024

DNA methylation plays a crucial role in development, aging, degeneration of various tissues and dedifferentiated cells. This review explores the multifaceted impact on retina brain during development pathological processes. First, we investigate retinal then focus diseases, detailing changes patterns diseases such as diabetic retinopathy (DR), age-related macular (AMD), glaucoma. Since is considered an extension brain, its unique structure allows it to exhibit similar immune response mechanisms brain. We further extend our exploration from examining associated Alzheimer's disease (AD) Huntington's (HD) better understand mechanistic links between explore possibility communication visual system central nervous (CNS) epigenetic perspective. Additionally, discuss neurodevelopmental including schizophrenia (SZ), autism spectrum disorder (ASD), intellectual disability (ID), how affects neuronal synaptic plasticity, cognitive function, providing insights into molecular underlying disorders.

Language: Английский

Citations

1