Association between the expression level of miRNA‑374a and TGF‑β1 in patients with colorectal cancer DOI Open Access
Noha G. Bader El Din, Reem El‐Shenawy,

Rehab I. Moustafa

et al.

World Academy of Sciences Journal, Journal Year: 2024, Volume and Issue: 6(6)

Published: Oct. 7, 2024

Colorectal cancer (CRC) poses a significant threat to global health with increasing incidence and mortality rates. Exploring different molecular mechanisms aspects underlying the development progression of CRC will promote early diagnosis lead better outcomes. MicroRNAs (miRNAs/miRs) are one related CRC. The present study aimed investigate association between expression levels miRNA‑374a transforming growth factor (TGF)‑β1 in patients explore roles these markers carcinogenesis their use as diagnostic biomarkers. For this purpose, 195 subjects were enrolled. level was assessed serum samples 145 (screening phase) addition 50 tissue validate tumor‑derived (confirmation phase). analysis performed by extraction miRNAs followed reverse transcription‑quantitative PCR. Additionally, TGF‑β1 measured all control samples. ROC curve determine potential TGF‑β1. exhibited significantly elevated compared healthy individuals, fold change 12.92 (P=0.0001). Moreover, varied, depending on grade; grade I disease 9.2‑fold increase those II 21.84‑fold expression. relative tissues upregulated 4.48‑fold adjacent non‑cancerous (P<0.001). Furthermore, controls area under 0.9560 for (P<0.0001), 0.8972 (P<0.0001) 0.8075 (P=0.001). In addition, positive correlation found miRNA‑374 (Rho=0.8912, P=<0.0001). On whole, demonstrates that indicate crucial driving tumor highlight biomarkers therapeutic targets

Language: Английский

The Immune Environment in Colorectal Adenoma: A Systematic Review DOI Creative Commons
Ugnė Šilinskaitė, Jurate Valciukiene, Matas Jakubauskas

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(3), P. 699 - 699

Published: March 12, 2025

Background/Objectives: Research on colorectal adenoma is significantly less comprehensive compared to studies carcinoma. Although a precursor of the majority sporadic cancers, not all adenomas develop into carcinomas. The complex interaction immune responses in premalignant tumor microenvironment might be factor for that. Methods: In this systematic review, we aim provide thorough analysis current research examining infiltration patterns tissues context cell-based, cytokine-based, and other immunological factor-related changes along conventional adenoma–carcinoma sequence. articles included review extend up December 2024 PubMed Web Science databases. Results: Most have shown significant differences cell counts, densities, cytokine expression levels associated with lesions (and/or cancer). No consensus immune-related tendencies concerning CD4+T cells CD8+T was reached. Decreasing mDCs plasma naïve B were detected ACS. increased density tissue eosinophils dramatically diminishes after transition As progresses, increasing IL-1α, IL-4, IL-6, IL-8, IL-10, IL-17A, IL-21, IL-23, IL-33, TGF-β decreasing IL-12A, IL-18, IFN—γ, TNFα cytokines invasive carcinoma stage being detected. over-expression COX-2, PD-1/PD-L1, CTLA-4, ICOS/ICOSLG adenomatous cancerous also observed. Conclusions: Further are needed better understanding whole picture adenoma-associated immunity its impact precancerous lesion’s potential progress.

Language: Английский

Citations

0
Unraveling BRAF alterations: molecular insights to circumvent therapeutic resistance across cancer types DOI Open Access

Caterina Perfetto,

Marianna Aprile, Simona Cataldi

et al.

Cancer Drug Resistance, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Aim: As intrinsic resistance - often driven by concurrent genomic alterations in tumor suppressor genes or oncogenes remains a major challenge oncology, this work aimed to comprehensively analyze BRAF somatic across cancer types and identify new potential therapeutic strategies overcome drug resistance. Methods: We conducted an extensive analysis of genomics, transcriptomics, clinical data retrieved from public repositories, including cBioPortal. Our comprehensive examined [point mutations, structural variants (SVs) copy number alteration] more than 217,000 samples 120 distinct primary metastatic sites both adult pediatric cohorts, focusing on mutual exclusivity co-occurrence mutations other suppressors. The also explores the association with survival, molecular features. Results: Analysis mutation frequencies revealed that BRAFV600E represents approximately 90% all alterations. While melanoma thyroid carcinoma show highest prevalence followed colorectal non-small cell lung terms absolute patients harboring worldwide, notably high were identified rare malignancies, hairy-cell leukemia, ganglioglioma, serous borderline ovarian tumors. profiling these tumors uncovered patterns co-occurring mutually exclusive genes, illuminating mechanisms suggesting novel combinations. Conclusion: Comprehensive is critical for optimizing targeted therapy overcoming -mutated cancers. identification provides opportunities rational combination therapies, emphasizing importance detailed developing effective treatment diverse types.

Language: Английский

Citations

0
Drugs Discovery from Traditional Phytotherapy DOI
Biswajit Patra,

Anil Biswal

Published: April 29, 2025

BACKGROUND. Phytotherapy refers to the use of plant extracts for therapeutic purpose. It plays important role in ancient medicine practices. has been considered as a cornerstone development modern drugs. With advancement field science and technology, researchers systematically evaluate medicinal properties plants. OBJECTIVE. is an integrative approach that integrates traditional knowledge with techniques identification, isolation, test derivative compounds. Literature on plant-derived natural products used suitable precursors drug discovery was included. METHODS. The data source were obtained through literature publications using different scientific search engines. RESULTS. These papers carefully evaluated, critically analyzed structured accurate information. process begins ethnobotanical surveys study select promising potential herb. Phytochemical methods are then employed extract isolate active constituents. phytotherapy drugs have plants support health manage various conditions. Recently analytical chemistry genomics essential accelerating process. High-throughput screening, molecular docking, bioinformatics tools enabled rapid identification bioactive Traditional significantly influenced by providing wealth about plant-based remedies. Many pharmaceuticals roots herbal practices, compounds isolated from forming basis effective treatments. CONCLUSION. This integration wisdom research led targeted therapies, emphasizing importance discovery. As continue explore compounds, remains vital new medicines. Moreover, advancements pharmacokinetics pharmacodynamics improved understanding interact biological systems, enhancing their efficacy safety profiles.

Language: Английский

Citations

0
CRISPR/Cas9-directed epigenetic editing in colorectal cancer DOI
Manoj Gupta,

Gayatri Gouda,

Maryam Moazzam‐Jazi

et al.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2025, Volume and Issue: unknown, P. 189338 - 189338

Published: April 1, 2025

Language: Английский

Citations

0
Exploring the molecular mechanisms and therapeutic potential of SMAD4 in colorectal cancer DOI Creative Commons

Hui Shan,

Guangyu Tian,

Yeqing Zhang

et al.

Cancer Biology & Therapy, Journal Year: 2024, Volume and Issue: 25(1)

Published: Aug. 20, 2024

Colorectal Cancer (CRC) is the third most common cancer worldwide, and occurrence development of CRC are influenced by molecular biology characteristics CRC, especially alterations in key signaling pathways. The transforming growth factor-β (TGF-β) plays a crucial role cellular growth, differentiation, migration, apoptosis, with SMAD4 protein serving as transcription factor TGF-β pathway, thus playing significant onset progression CRC. one malignancies high mortality rate worldwide. Despite research progress recent years, regarding SMAD4, its dual early late stages tumor has promoted further discussion on complexity therapeutic target, highlighting urgent need for deeper analysis This review aims to explore function potential target.

Language: Английский

Citations

3
Association between the expression level of miRNA‑374a and TGF‑β1 in patients with colorectal cancer DOI Open Access
Noha G. Bader El Din, Reem El‐Shenawy,

Rehab I. Moustafa

et al.

World Academy of Sciences Journal, Journal Year: 2024, Volume and Issue: 6(6)

Published: Oct. 7, 2024

Colorectal cancer (CRC) poses a significant threat to global health with increasing incidence and mortality rates. Exploring different molecular mechanisms aspects underlying the development progression of CRC will promote early diagnosis lead better outcomes. MicroRNAs (miRNAs/miRs) are one related CRC. The present study aimed investigate association between expression levels miRNA‑374a transforming growth factor (TGF)‑β1 in patients explore roles these markers carcinogenesis their use as diagnostic biomarkers. For this purpose, 195 subjects were enrolled. level was assessed serum samples 145 (screening phase) addition 50 tissue validate tumor‑derived (confirmation phase). analysis performed by extraction miRNAs followed reverse transcription‑quantitative PCR. Additionally, TGF‑β1 measured all control samples. ROC curve determine potential TGF‑β1. exhibited significantly elevated compared healthy individuals, fold change 12.92 (P=0.0001). Moreover, varied, depending on grade; grade I disease 9.2‑fold increase those II 21.84‑fold expression. relative tissues upregulated 4.48‑fold adjacent non‑cancerous (P<0.001). Furthermore, controls area under 0.9560 for (P<0.0001), 0.8972 (P<0.0001) 0.8075 (P=0.001). In addition, positive correlation found miRNA‑374 (Rho=0.8912, P=<0.0001). On whole, demonstrates that indicate crucial driving tumor highlight biomarkers therapeutic targets

Language: Английский

Citations

0