Methylglyoxal-Stimulated Mesothelial Cells Prompted Fibroblast-to-Proto-Myofibroblast Transition DOI Open Access
Yu-Syuan Wei, Su‐Yi Tsai, Shuei‐Liong Lin

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 813 - 813

Published: Jan. 19, 2025

During long-term peritoneal dialysis, fibrosis (PF) often happens and results in ultrafiltration failure, which directly leads to the termination of dialysis. The accumulation extracellular matrix produced from an increasing number myofibroblasts was a hallmark characteristic PF. To date, glucose degradation products (GDPs, i.e., methylglyoxal (MGO)) that appeared during heating storage dialysate are considered be key components initiating PF, but how GDPs lead activation myofibroblast fibrotic peritoneum has not yet been fully elucidated. In this study, mesothelial cell line (MeT-5A) fibroblast (MRC-5) were used investigate transcriptomic proteomic changes unveil underlying mechanism MGO-induced Our data MGO-stimulated cells showed upregulation genes involved pro-inflammatory, apoptotic, pathways. While no phenotypic noted on fibroblasts after direct MGO, supernatant promoted change into proto-myofibroblasts, activated first stage toward myofibroblasts. conclusion, study fibroblast-to-proto-myofibroblast transition; however, additional involvement other factors or (e.g., macrophages) may needed complete transformation

Language: Английский

Methylglyoxal-Stimulated Mesothelial Cells Prompted Fibroblast-to-Proto-Myofibroblast Transition DOI Open Access
Yu-Syuan Wei, Su‐Yi Tsai, Shuei‐Liong Lin

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 813 - 813

Published: Jan. 19, 2025

During long-term peritoneal dialysis, fibrosis (PF) often happens and results in ultrafiltration failure, which directly leads to the termination of dialysis. The accumulation extracellular matrix produced from an increasing number myofibroblasts was a hallmark characteristic PF. To date, glucose degradation products (GDPs, i.e., methylglyoxal (MGO)) that appeared during heating storage dialysate are considered be key components initiating PF, but how GDPs lead activation myofibroblast fibrotic peritoneum has not yet been fully elucidated. In this study, mesothelial cell line (MeT-5A) fibroblast (MRC-5) were used investigate transcriptomic proteomic changes unveil underlying mechanism MGO-induced Our data MGO-stimulated cells showed upregulation genes involved pro-inflammatory, apoptotic, pathways. While no phenotypic noted on fibroblasts after direct MGO, supernatant promoted change into proto-myofibroblasts, activated first stage toward myofibroblasts. conclusion, study fibroblast-to-proto-myofibroblast transition; however, additional involvement other factors or (e.g., macrophages) may needed complete transformation

Language: Английский

Citations

0