Parishin B blocking TRIB3-AKT1 interaction inhibits breast cancer lung metastasis

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 15, 2025

TRIB3 has been reported to mediate breast cancer (BC) proliferation and metastasis by interacting with AKT1, blocking the interaction between AKT1 can inhibit progression of BC. Besides, inhibiting turn "cold tumor" hot also proved be an effective therapeutic strategy for Thus, this study aim find drugs that bind BC progression, further elucidate its mechanism. The possible inhibitors were screened high-throughput molecular docking, CETSA, CO-IP assay. Then, effect inhibitor anti was assessed CCK-8 assay, flow cytometry, plate colony formation transwell assay; RNA-seq empolyed potential mechanism Parishin B (PB) anti-BC. Finally, on lung in vivo evaluated. PB as a TRIB3, CETSA assay indicated could target block TRIB3-AKT1 interaction. In addition, exhibited good anti-BC activity without drug toxicity normal cells experiments vitro, analysis suggested invasion related cell cycle. It vivo. demonstrated regulating cycle, providing agent treatment

Language: Английский

Mechanism of Rabdosia rubescens extract against gastric cancer microenvironment by SIRT1/NF-κB/p53 pathway and promoting tumor-associated macrophage polarization

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119935 - 119935

Published: May 1, 2025

Language: Английский

CYTOTOXIC AND APOPTOTIC EFFECT OF Vernonia amygdalina Delile. FRACTIONS AGAINST Hs578t TRIPLE-NEGATIVE BREAST CANCER CELL LINES

Phytomedicine Plus, Journal Year: 2024, Volume and Issue: unknown, P. 100640 - 100640

Published: Sept. 1, 2024

Language: Английский