Closing loops in intensive livestock systems: innovative strategies for nutrient recycling and emissions reduction DOI Creative Commons

Lluís Morey Gual

Published: Nov. 17, 2023

(English) The exponential growth of world population is leading to a high demand food products and, consequently, the development intensive agricultural and livestock systems. In this sense, synthetic fertilizers are being used in non-sustainable way produce feedstock, requiring massive amounts energy depleting mineral resources, with volatile fluctuation their prices due geopolitical conflicts. At same time, production still inefficient terms nutrient use contributes half amount greenhouse gases most ammonia emissions agriculture. context, recovery biobased bioenergy from animal manure partially replace fossil fuels, as well strategies reduce along management chain, should be considered key approach move towards more sustainable resilient agriculture production. This doctoral thesis has looked contribute aim by developing different innovative at two main levels: (i) level, precision feeding tools have been adjust diets dairy cows based on individual requirements, reducing nitrogen present excreta, potential volatilization ammonia. Nitrogen was reduced urine 28% 19% respectively without affecting milk production, reduction trend emission 20% during storage observed. Besides, samples cow ruminal fluid were obtained isolate identify hyper ammonia-producing bacteria that highly cows’ metabolism. After identification Clostridium sporogens, Terrisporobacter glycolicus (Clostridium glycolicum), Megasphaera elsdenii, argentinense, Streptococcus sp., Prevotella ruminicola, Acidaminococcus fermentans, an vitro study using bacteriophages isolated inoculated strategy. Unfortunately, none rumen able interact bacteria. (ii) treatment valorization agro-industrial anaerobic digestate, solid fraction mixture low stream stripping/scrubbing unit dried solar drying system, some cases combined acidification sulfuric acid, organic NPK fertilizers, while gas process. All products, both acidified non-acidified, meet current European Regulation for exceptions zinc content. Moreover, proved up 94% process when comparing non-acidified products. addition, resulting tested phytotoxicity germination plant pot trials lettuce. Except rest did not any toxicity effect germination, digestate showed biostimulator (Germination index > 120%). During trials, efficient performance, always lower than fertilizer, but similar case better negative control. them fit regulations heavy metals toxic elements soil edible parts plant. (Català) El creixement exponencial de la població mundial està portant una gran demanda productes alimentaris i, en conseqüència, al desenvolupament sistemes agrícoles i ramaders intensius. En aquest sentit, els fertilitzants sintètics s'estan utilitzant manera no sostenible per produir aliments matèries primeres, que requereixen quantitats massives d'energia esgoten recursos minerals, amb fluctuació volàtil dels seus preus conflictes geopolítics. Al mateix temps, ramaderia intensiva és encara ineficient pel fa l'ús nutrients contribueix meitat quantitat gasos d'efecte hivernacle majoria les d'amoníac l’agricultura. recuperació biològics bioenergia partir dejeccions animals substituir parcialment minerals combustibles fòssils, així com estratègies reduir llarg cadena gestió dejeccions, s'haurien considerar un enfocament clau avançar cap sistema més sostenible, agricultura producció ramadera resilients. Aquesta tesi ha volgut contribuir objectiu desenvolupant diferents innovadores dos nivells principals: A nivell animal, s'han utilitzat eines d'alimentació precisió ajustar dietes vaques lleteres funció necessitats individuals animals, reduint excretes possible volatilització l'amoníac. es va l'orina 19%, respectivament, sense afectar llet, observar tendència reducció del durant l'emmagatzematge dejeccions. més, van obtenir mostres líquid aïllar identificar bacteris híper-productors d’amoníac contribueixen substancialment el metabolisme vaques. Després identificació ruminicola fer estudi inocular bacteriòfags estratègia població. Malauradament, presents nostre poder interactuar bacteris. tractament valorització assecar digestat agroindustrial, fracció sòlida barreja corrent baixa procedent d’una unitat stripping d’assecat solar, alguns casos combinats acidificació àcid sulfúric, orgànics NPK, alhora reduïen d’efecte procés. Tots secs, tant acidificats acidificats, compliren Reglament europeu vigent sòlids algunes excepcions contingut zinc. l’acidificació demostrar fins procés comparar acidificats. Els resultants ser provats experiment germinació assajos test enciam determinar seva fitotoxicitat. Excepte sec acidificat, resta efecte toxicitat germinació, acidificat mostrar bioestimulador (índex Durant proves test, rendiment eficient, sempre inferior sintètic, però cas millor control negatiu. ells s’ajustaren normativa sobre metalls pesants potencialment tòxics sòl comestibles planta. (Español) crecimiento población está potenciando productos alimenticios y, consecuencia, desarrollo sistemas agrícolas y ganaderos intensivos. este sentido, los fertilizantes sintéticos se están utilizando forma para producir alimentos materias primas, requieren cantidades masivas energía agotan minerales, con fluctuación volátil sus precios por conflictos geopolíticos. mismo tiempo, ganadería todavía ineficiente lo refiere uso nutrientes contribuye mitad cantidad efecto invernadero ya mayoría las emisiones amoníaco agricultura. contexto, recuperación biológicos bioenergía estiércoles animales sustituir parcialmente minerales fósiles, así como estrategias reducir largo gestión estiércol, deberían enfoque clave avanzar hacia más producción ganadera resilientes. Esta tesis querido objetivo desarrollando diferentes innovadoras niveles principales: nivel han utilizado herramientas alimentación precisión dietas vacas lecheras función necesidades individuales animales, reduciendo nitrógeno presente excretas posible volatilización amoníaco. redujo orina heces respectivamente, sin leche, observó tendencia reducción durante almacenamiento estiércol. Además, obtuvieron muestras líquido aislar e bacterias híper-productoras contribuyen sustancialmente metabolismo vacas. Tras identificación realizó estudio inocularon bacteriófagos ruminal. Desgraciadamente, ninguno presentes nuestro pudo bacterias. tratamiento valorización secaron digestato fracción sólida mezcla corriente baja procedente unidad secado algunos combinados acidificación ácido sulfúrico, orgánicos tiempo reducían proceso. Todos secos, tanto acidificados acidificados, cumplieron Reglamento europeo vigente sólidos algunas excepciones contenido demostró hasta proceso acidificados. Los resultantes fueron probados experimento germinación ensayos maceta lechuga su fitotoxicidad. Excepto seco acidificado, resto produjeron ningún toxicidad t acidificado mostró (índice Durante pruebas mostraron rendimiento eficiente, siempre sintético pero caso mejor negativo. ellos ajustaron metales pesados elementos potencialmente tóxicos.

Language: Английский

Experimental evaluation and optimization of the anaerobic digestibility of two new desert weeds for biogas production DOI
Mohammad Gholizadeh, Mahdi Deymi‐Dashtebayaz,

Abolfazl Mehri

et al.

Biomass Conversion and Biorefinery, Journal Year: 2022, Volume and Issue: 14(7), P. 8395 - 8405

Published: June 6, 2022

Language: Английский

Citations

9

Effectiveness of precision feeding in reducing N excretion in dairy cattle DOI Creative Commons
Lluís Morey, À. Bach,

Daniel Sabrià

et al.

Animal Feed Science and Technology, Journal Year: 2023, Volume and Issue: 304, P. 115722 - 115722

Published: July 1, 2023

Two periods enrolling 56 (milk yield 36.2 ± 7.91 kg/d) and 58 32.4 4.99 grouped-fed dairy cows were performed to evaluate nutrient adjustment N emissions of a precision feeding strategy. Pens blocked by parity randomly assigned conventional (CONV) or scheme (PREC) for 21-d period. The CONV group was offered total mixed ration (TMR, 6.82 6.65 MJ NEl/kg DM, in period 1 2, respectively, 165 g CP/kg DM both periods; whereas PREC fed partial (PMR, 6.40 135 137 respectively) concentrate feed supplemented twice daily the milking parlour, which contained different quantities soybean meal, corn wheat middling's according estimated nutritional needs each cow above those supplied consumption PMR. Individual nutrients consumed from PMR calculated using 10-d rolling average performance data concentration its components, BW recorded periods). A balance urine fecal spot sampling during last 3 d study 1, stored manure gaseous (ammonia, methane, nitrous oxide, carbon dioxide) measured 2 wk 2. After adaptation diet, 82 homogeneously distributed 4 DIM categories: early (< 81), mid-early (81 150), mid-late (151 220), late (> 220) used assess how energy protein requirements adjusted system. Dairy systems energetically overfed, tended be more CP overfed than cows. Total excretion, milk urea greater There no differences ammonia oxide storage between cows; however, methane dioxide increased 55 15%, respectively Precision system based on preceding composition can reduce excretion without affecting short-term but increasing C manure.

Language: Английский

Citations

5

Integrative microbial and transcriptomic analysis reveals the lignocellulosic biomass degrading mechanism by bamboo snout beetle DOI
Hao Tang, Yuanqiu Li, Mingjun Wang

et al.

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 203, P. 117194 - 117194

Published: July 21, 2023

Language: Английский

Citations

5

Optimisation of Anaerobic Digestate and Chemical Fertiliser Application to Enhance Rice Yield—A Machine-Learning Approach DOI Open Access
Binoy Kumar Show, Suraj Panja, Richik GhoshThakur

et al.

Sustainability, Journal Year: 2023, Volume and Issue: 15(18), P. 13706 - 13706

Published: Sept. 14, 2023

The present study evaluates the synergistic application of an anaerobic digestate for enhanced rice yield. utilised as a fertiliser with various inoculum-to-substrate (IS) ratios digestion from cow dung and water hyacinth (CW–BF) combinations NPK (16-22-22) yield optimisation. outcome combined on cultivation was observed in terms parameters such number tillers, panicle number, length, fertile panicles, 1000-grain weight. combination CW–BF:NPK (3:1:1) resulted highest grain (7521 kg/hectare) increased test weight, more filled grains than other combinations. Moreover, machine-learning approaches were used to efficacy different applied (cow dung, hyacinth, NPK). gradient-boosting model appropriate predicting modelling based measured data. Principal component analysis revealed first principal high loading values second component, which indicates its crucial role preparation. Therefore, deploying hybridised fertilisers using proper statistical can improve yield, would be essential socio-economic uplifting marginal farmers.

Language: Английский

Citations

4

Synergistic effect of biological pre-treatment on co digestion of rice straw and sewage sludge: Process optimization and microbial interactions DOI

Vijayalakshmi Arelli,

V. V. Basava Rao,

Naveen Kumar Mamindlapelli

et al.

Biocatalysis and Agricultural Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 103364 - 103364

Published: Sept. 1, 2024

Language: Английский

Citations

1

Screening for suitable mixed microbial consortia from anaerobic sludge and animal dungs for biodegradation of brewery spent grain DOI Creative Commons
Juan Castilla-Archilla, Camilla Thorn, Simone Pau

et al.

Biomass and Bioenergy, Journal Year: 2022, Volume and Issue: 159, P. 106396 - 106396

Published: March 2, 2022

Lignocellulosic wastes, such as brewery spent grain (BSG), represent valuable resources from a biorefinery perspective, being sources of both chemical and energy production. Improving degradation rates these recalcitrant feedstocks is necessity, bioaugmentation with specialised microbial consortia can be viable means achieving this. Six mixed were screened, using biodegradation activity tests, for their capacity to enhance production chemicals (volatile fatty acids or ethanol) (biogas) BSG. The inocula used anaerobic granular sludge i) intact granules ii) crushed granules, iii) bovine rumen fluid, number animal manures including iv) giraffe, v) rhinoceros, vi) tiger. Amplicon sequencing was investigate prokaryotic communities within on day 0 21. Despite herbivore dung harbouring the largest hydrolytic community, best solids destruction achieved sludge, either (64.8 ± 11.9%) (63.4 3.7% VS destruction). Granular also resulted in highest gas production, followed by giraffe manure. Contrastingly, volatile acid accumulation rhinoceros tiger manure, at 3.86 (±0.55) 3.20 (±0.74) gCOD L−1, respectively. two manure samples having relative abundances bacteria associated cellulose hemicellulose degradation, they supported poorest destruction, perhaps due VFA therein. Thus, while some aided BSG further research required determine inhibitors hydrolysis therefore degradation.

Language: Английский

Citations

6

Five Himalayan weeds as potential bioresources for bioactive agents: toxic compounds to valuable scaffolds DOI
Ekta Bala, Varun Aggarwal, Pawan Kumar

et al.

Phytochemistry Reviews, Journal Year: 2024, Volume and Issue: 23(4), P. 1205 - 1246

Published: Feb. 14, 2024

Language: Английский

Citations

0

Technological Advancement for Biohydrogen Production from Agricultural Waste DOI
Anudeb Ghosh, Apurba Koley,

Saradashree Pal

et al.

Clean Energy Production Technologies, Journal Year: 2024, Volume and Issue: unknown, P. 175 - 221

Published: Jan. 1, 2024

Language: Английский

Citations

0

Optimization of culture medium in the production of cellulolytic enzyme by Klebsiella variicola isolated from termite gut in Indralaya peatlands, Indonesia DOI Open Access
Oktiarni Dwita, Hermansyah Hermansyah, Eddy Ibrahim

et al.

AIP conference proceedings, Journal Year: 2024, Volume and Issue: 3080, P. 040002 - 040002

Published: Jan. 1, 2024

Bioethanol as an alternative energy resource, has become essential to be developed eco-environmentally energy. Enzymatic processes can used for cellulose biomass generate bioethanol. Termite is the organism that consumes a food source. In this study, cellulolytic Klebsiella variicola isolated from termite gut were optimized increase enzyme production. Several factors affect growth and production of cellulase enzymes. research, optimization culture medium was examined using different carbon sources, various concentration diverse nitrogen pH, temperature, agitation. The result showed optimal with higher activity obtained in addition source fructose 3.5%; sodium nitrate 2%; pH 5; temperature 45°C; agitation 150 rpm.

Language: Английский

Citations

0

Draft genome sequence data on Bacillus safensis U41 isolated from soils of Santiniketan, India DOI Creative Commons
Binoy Kumar Show,

Andrew B. Ross,

Raju Biswas

et al.

Data in Brief, Journal Year: 2024, Volume and Issue: 54, P. 110547 - 110547

Published: May 21, 2024

The draft genome sequence of an isolate Bacillus safensis U41 from the soils Santiniketan (23040'12" N and 87039'52" E) is reported here. a bacterium that produces cellulases which essential for breakdown plant biomass. As such, it valuable source digestive enzymes biomass, especially cellulases. genomic DNA was extracted single colony using QIAgen Blood Tissue kit (QIAgen Inc., Canada). Sequencing performed via Illumina HiSeq X 2 × 150 paired-end chemistry, generating 7,352,576 reads with coverage 509x. assembly produced 20 contigs over 200 base pairs (bp) in length, N50 value 901304 L50 2. size 3,732,407 bp, average GC content 41.43 %. Genome annotation gene predictions were Prokka v.1.14.6, identified 3783 coding sequences, 64 tRNA genes, 3 rRNA genes.

Language: Английский

Citations

0