Published: Jan. 1, 2023

Despite benefits such as lower water and working volume requirements, thermophilic high solids anaerobic digestion (HSAD) often fails due to the rapid build-up of volatile fatty acids (VFA) associated drop in pH. Use conductive materials can promote HSAD through stimulation direct interspecies electron transfer (DIET), while need for their constant dosing impairs economic feasibility. In this work, a two-phase one-reactor system amended with granular activated carbon (GAC) or magnetite was used organic fraction municipal solid waste (OF-MSW). Both GAC addition could alleviate inhibition after VFA ~28-30 g/L. The highest methane yield (286 mL CH4/g VS) achieved 20 g magnetite-added reactors, maximum CH4 production rates (26.38 VS/d) lowest lag-phase (2.83 days) were obtained GAC-added reactors. occurrence pili-like structures on surface suggests that A-type DIET actively proceeded these biofilms. enrichment different syntrophic potentially electroactive microbial groups (Lentimicrobiaceae, Hydrogenispora, Clostridia MBA03, Defluviitoga) (Ruminiclostridium 1, Clostridium Defluviitoga, Defluviitalea, Lentimicrobiaceae) biofilms indicates existence specific preferences nature material. Prediction basic metabolic functions has shown increase abundances cellular processes signals, lipid transport metabolism, resulting enhanced production. Thus, amendment leads structural functional rearrangements initial community. Promising results work spatial separation bulk OF-MSW materials, be useful modelling continuous acidogenic-methanogenic systems good recovery materials.

Language: Английский