Harnessing genetic engineering to drive economic bioproduct production in algae

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 29, 2024

Our reliance on agriculture for sustenance, healthcare, and resources has been essential since the dawn of civilization. However, traditional agricultural practices are no longer adequate to meet demands a burgeoning population amidst climate-driven challenges. Microalgae emerge as beacon hope, offering sustainable renewable source food, animal feed, energy. Their rapid growth rates, adaptability non-arable land non-potable water, diverse bioproduct range, encompassing biofuels nutraceuticals, position them cornerstone future resource management. Furthermore, microalgae’s ability capture carbon aligns with environmental conservation goals. While microalgae offers significant benefits, obstacles in cost-effective biomass production persist, which curtails broader application. This review examines compared other host platforms, highlighting current innovative approaches aimed at overcoming existing barriers. These include range techniques, from gene editing, synthetic promoters, mutagenesis selective breeding metabolic engineering through transcription factors.

Language: Английский

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Trends and advances in sustainable bioethanol production technologies from first to fourth generation: A critical review

Energy Conversion and Management, Journal Year: 2024, Volume and Issue: 321, P. 119037 - 119037

Published: Sept. 21, 2024

Language: Английский

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Landscape of microalgae omics and metabolic engineering research for strain improvement: An overview

Aquaculture, Journal Year: 2024, Volume and Issue: 587, P. 740803 - 740803

Published: March 16, 2024

The unique metabolic capabilities and fast growth rates of microalgae render them promising candidates for various industrial applications, such as biofuel production, pharmaceuticals, nutraceuticals, wastewater treatment. Metabolic engineering is a powerful approach used to enhance the sustainable production high-value compounds in microalgae, improve their stress tolerance, characteristics suitability large-scale cultivation. This review provides snapshot current state knowledge on omics research further our understanding microalgal metabolism enable development optimized strains with improved productivity functionality. More specifically, it focuses recent breakthroughs omics, driven by advancements genomics technologies, sequencing platforms bioinformatics tools, that have enabled functional characterization key genes, identification pathways, elucidation cell physiology. Conventional state-of-the-art genetic approaches last decades manipulate pathways targeted manner, are highlighted scope optimization. In this review, different applications impact industry also discussed. Integrating pan-omics data future crucial predicting novel interactions identifying aspects flux, towards enhancing algal strain-engineering techniques.

Language: Английский

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CRISPR-based bioengineering in microalgae for production of industrially important biomolecules

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Oct. 26, 2023

Microalgae, as photosynthetic organisms, have the potential to produce biomolecules for use in food, feed, cosmetics, nutraceuticals, fuel, and other applications. Faster growth rates higher protein lipid content make microalgae a popular chassis many industrial However, challenges such low productivity high production costs limited their commercialization. To overcome these challenges, bioengineering approaches genetic engineering, metabolic synthetic biology been employed improve quality of microalgae-based products. Genetic engineering employing genome editing tools like CRISPR/Cas allows precise targeted modifications. systems are presently used modify makeup enhanced specific biomolecules. yet be explored explicitly owing some limitations. Despite progress made CRISPR-based approaches, there is still need further research optimize This includes improving efficiency tools, understanding regulatory mechanisms microalgal metabolism, optimizing conditions cultivation strategies. Additionally, addressing ethical, social, environmental concerns associated with modification crucial responsible development commercialization review summarizes advancements industrially important provides key considerations microalgae. The will help researchers understand initiate experiments

Language: Английский

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Advances in algal lipid metabolism and their use to improve oil content

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 87, P. 103130 - 103130

Published: April 4, 2024

Language: Английский

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High‐efficiency genome editing by Cas12a ribonucleoprotein complex in Euglena gracilis

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(2)

Published: Feb. 1, 2024

Abstract Transgene‐free genome editing based on clustered regularly interspaced short palindromic repeats (CRISPR) technology is key to achieving genetic engineering in microalgae for basic research and industrial applications. Euglena gracilis , a unicellular phytoflagellate microalga, promising biomaterial foods, feeds, cosmetics biofuels. However, methods the manipulation of E. are still limited. Here, we developed high‐efficiency, transgene‐free method using Lachnospiraceae bacterium CRISPR‐associated protein 12a (LbCas12a) ribonucleoprotein (RNP) complex, which complements previously established Cas9 RNP‐based method. Through direct delivery LbCas12a‐containing RNPs, our reached mutagenesis rates approximately 77.2–94.5% at two different target genes, Glucan synthase‐like 2 ( EgGSL2 ) phytoene synthase gene EgcrtB ). Moreover, addition targeted mutagenesis, demonstrated efficient knock‐in base site LbCas12a‐based RNPs with single‐stranded DNA donor template gracilis. This study extends capabilities accelerate its use bioproduction.

Language: Английский

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Molecular design of microalgae as sustainable cell factories

Trends in biotechnology, Journal Year: 2023, Volume and Issue: 42(6), P. 728 - 738

Published: Dec. 12, 2023

Microalgae are regarded as sustainable and potent chassis for biotechnology. Their capacity efficient photosynthesis fuels dynamic growth independent from organic carbon sources converts atmospheric CO

Language: Английский

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Engineering of ketocarotenoid biosynthetic pathway in Chlamydomonas reinhardtii through exogenous gene expression

Systems Microbiology and Biomanufacturing, Journal Year: 2024, Volume and Issue: 4(3), P. 983 - 995

Published: March 20, 2024

Language: Английский

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Microalgae growth-promoting bacteria for cultivation strategies: Recent updates and progress

Microbiological Research, Journal Year: 2024, Volume and Issue: 286, P. 127813 - 127813

Published: June 22, 2024

Language: Английский

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Genetic Improvement of Kappaphycus alvarezii (Doty) by Molecular Breeding

Published: Jan. 1, 2025

Language: Английский

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