Efficient production of guanosine in Escherichia coli by combinatorial metabolic engineering DOI Creative Commons
Kun Zhang,

Mengxing Qin,

Yu Hou

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 13, 2023

Abstract Guanosine is a purine nucleoside that widely used as raw material for food additives and pharmaceutical products. However, the lack of strains with efficiently producing guanosine greatly limited industrial application. We attempted to produce guanosinein Escherichia coli using systematic metabolic engineering. First, we overexpressed synthesis pathway from Bacillus subtilis the prs gene, deleted three genes involved in guanosinecatabolism increase accumulation. Subsequently, attenuated purA expression eliminated feedback inhibition. Then, modified flux glycolysis Entner-Doudoroff pathways performed redox cofactors rebalancing. Finally, transporter engineering further increased titre 123.6 mg/L. After 72 h fed-batch fermentation shake-flask, achieved 145.2 Our results reveal guanosinesynthesis was successfully optimized by combinatorial engineering, which could be applicable efficient other

Language: Английский

The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction DOI Creative Commons

Xingyu Wu,

Tingting Zhang, Ke Zhang

et al.

Synthetic and Systems Biotechnology, Journal Year: 2024, Volume and Issue: 9(2), P. 277 - 284

Published: March 4, 2024

Aspergillus niger is an efficient cell factory for organic acids production, particularly l-malic acid, through genetic manipulation. However, the traditional method of collecting A. spores inoculation labor-intensive and resource-consuming. In our study, we used CRISPR-Cas9 system to replace promoter brlA, a key gene in conidiation, with xylose-inducible xylP acid-producing strain RG0095, generating brlAxylP. When induced xylose submerged liquid culture, brlAxylP exhibited significant upregulation conidiation-related genes. This induction allowed us easily collect abundance (>7.1 × 106/mL) medium. Significantly, conidiation approach preserves substantial potential as foundational cellular platform biosynthesis acids, including but not limited acid. summary, study offers simplified strategy streamline preparation stage reduce labor material costs industrial acid production using species.

Language: Английский

Citations

1
Engineering the by-products pathway in Aureobasidium pullulans for highly purified polymalic acid fermentation with concurrent recovery of l-malic acid DOI
Bingqin Li, Jinzhao He, Kangjia Zuo

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 414, P. 131578 - 131578

Published: Oct. 9, 2024

Language: Английский

Citations

1
Mycotoxin production by Fusarium species and a recent deep insight into management through biocontrol approaches DOI Creative Commons
Amjad Ali, Fatih Ölmez, Muhammed Tatar

et al.

Journal of Global Innovations in Agricultural Sciences, Journal Year: 2023, Volume and Issue: 11(4), P. 455 - 480

Published: Dec. 22, 2023

Fusarium infection has become a significant and widespread concern in modern agriculture, posing substantial threat to numerous essential crop plants. The pernicious fungal pathogen been causing destruction diverse areas, resulting severe consequences for both food security economic stability. Mycotoxins, which are secondary metabolites produced by wide variety of fungi, present serious risks the health humans animals have potential result considerable financial losses agricultural industries. genus is particularly infamous its capacity produce range mycotoxins. summarized study highlights recent progression fusarium towards various pivotal plants with their life cycle, morphological microscopic characteristics, pathogenicity factors. species well-known ability mycotoxins, such as zearalenones, fumonisins, Trichothecenes, Deoxynivalenol, Nivalenol, T-2 toxins detrimental that contaminate products, particular emphasis on grains cereals. new emerging mycotoxins producing challenges control human health. identification implementation efficient biocontrol strategies detoxify through Trichoderma, Plant Secondary Metabolites, Lactic Acid Bacteria, Edible Mushrooms, Enzymes measures guaranteeing safety protecting public mentioned approaches also discussed addressed. Keywords: factors, trichothecenes, agents, enzymatic detoxification.

Language: Английский

Citations

1
Current Progress on Microbial l-malic Acid Production DOI Creative Commons

Lu Mou,

Min Qiu, Wankui Jiang

et al.

Synthetic biology and engineering, Journal Year: 2024, Volume and Issue: 2(2), P. 10010 - 10010

Published: Jan. 1, 2024

As an important intermediate in the tricarboxylic acid (TCA) cycle, l-malic (l-MA) is also one of 12 platform bulk chemicals with high added value. Owing to its various applications food, pharmaceuticals, cosmetics and industry, global l-MA market size growing year by year. Over last few decades, increasing concerns regarding fossil fuels depletion excessive CO2 emissions have led commitment fostering a green economy sustainable development. Alternatively, microbial fermentation has gradually attracted more attention. Here, this review summarizes common biosynthesis pathways compares differences between different chassis microorganisms as well. Moreover, regulation strategies genetic metabolic engineering process boost production are summarized, research status from cheaper substrates discussed. Finally, direction further exploration industrialized proposed, which provides theoretical guidance on promoting technological innovation industrial production.

Language: Английский

Citations

0
None DOI Creative Commons
Amjad Ali, Muhammed Tatar,

Irum T abbasum

et al.

Journal of Global Innovations in Agricultural and Social Sciences, Journal Year: 2014, Volume and Issue: 2(3)

Published: Sept. 1, 2014

countries, causing devastating impacts on timber trees in the forest (Suga and Hyakumachi, 2004).Fusarium species are associated swith production of different kinds secondary metabolites, such as gibberellin zearalenone, which most significant plant growth regulators also used to accelerate animals (Nesic Nesic, 2013;Qiu et al., 2020).Trichothecenes fumonisins produced by these pathogenic humans can cause mycotoxicosis when they fed contaminated food (Hao 2022; Li 2020).Fusarium spp.can survive dead decaying material, sustain itself soil for up 16 years absence a host, acts an important biodegrading agent (Early 2009).

Language: Английский

Citations

0
Efficient production of guanosine in Escherichia coli by combinatorial metabolic engineering DOI Creative Commons
Kun Zhang,

Mengxing Qin,

Yu Hou

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 13, 2023

Abstract Guanosine is a purine nucleoside that widely used as raw material for food additives and pharmaceutical products. However, the lack of strains with efficiently producing guanosine greatly limited industrial application. We attempted to produce guanosinein Escherichia coli using systematic metabolic engineering. First, we overexpressed synthesis pathway from Bacillus subtilis the prs gene, deleted three genes involved in guanosinecatabolism increase accumulation. Subsequently, attenuated purA expression eliminated feedback inhibition. Then, modified flux glycolysis Entner-Doudoroff pathways performed redox cofactors rebalancing. Finally, transporter engineering further increased titre 123.6 mg/L. After 72 h fed-batch fermentation shake-flask, achieved 145.2 Our results reveal guanosinesynthesis was successfully optimized by combinatorial engineering, which could be applicable efficient other

Language: Английский

Citations

0