Cited by Dual-domain superoxide dismutase: In silico prediction directed combinatorial mutation for enhanced robustness and catalytic efficiency

Reconstruction of a microbial TNT deep degradation system and its mechanism for reshaping microecology DOI

Xu Yang,

Yongbing Zhu,

Sanping Zhao

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 137411 - 137411

Published: Jan. 1, 2025

Language: Английский

Citations

Characterization of 2-phenanthroyl-CoA reductase, an ATP-independent type III aryl-CoA reductase involved in anaerobic phenanthrene degradation DOI

Nadia A. Samak,

Fritz Götz,

Khadija Adjir

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

ABSTRACT Anaerobic degradation of polycyclic aromatic hydrocarbons (PAHs) with three or more rings is extremely slow because the compounds are very poorly soluble in water and chemically stable. Phenanthrene only three-ring PAH where anaerobic has been partially elucidated. first activated via carboxylation producing 2-phenanthroate, which further converted to 2-phenanthroyl-coenzyme A (CoA) enzyme 2-phenanthroate:CoA ligase. In this study, we elucidated next step, reduction 2-phenanthroyl-CoA dihydro-2-phenanthroyl-CoA. We cloned putative gene from genome culture TRIP_1 heterologously expressed purified reductase Escherichia coli . The identified monomeric flavo-enzyme belongs novel group type III aryl-CoA reductases old-yellow family a molecular mass 72 kDa. 2-Phenanthroyl-CoA contains one FMN, FAD, [4Fe-4S] iron-sulfur cluster as cofactors. specific activity 17.6 ± 0.4 nmol/min/mg, K m value 1.8 µM, Vmax 7.9 µmol/min/mg at pH 7.5, when reduced methyl viologen was used electron donor. catalyzed two-electron step five possible isomers. Quantum mechanical calculations nuclear magnetic resonance analysis reaction product suggested 9,10-dihydro-2-phenanthroyl-CoA most stable isomer. However, our experimental evidence suggests 7,8-dihydro-2-phenanthroyl-CoA (International Union Pure Applied Chemistry [IUPAC]: 1,2-dihydro-7-phenanthroyl-CoA) 5,6-dihydro-2-phenanthroyl-CoA (IUPAC: 3,4-dihydro-7-phenanthroyl-CoA) likely saturated bond ring 3 substrate 2-phenanthroyl-CoA, before undergoing isomerization changes reach structure 9,10-dihydro-2-phenanthroyl-CoA. IMPORTANCE PAHs highly toxic persistent environmental pollutants. like phenanthrene still understood. starts form 2-phenanthroic acid followed by CoA-thioesterification ligase produce 2-phenanthroyl-CoA. dihydro-2-phenanthroyl-CoA overcome energy system. Herein, that reductase. Furthermore, provided biochemical structural properties reductase, confirmed family.

Language: Английский

Citations

Toxicity evaluation and degradation of cypermethrin-contaminated soil using biochar and Bacillus cereus amendments DOI

Hamid Rehman,

Ziafat Rehman,

Tonoy K. Das

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 2, 2024

Cypermethrin (Cyp), a persistent synthetic pyrethroid insecticide widely used for insect control. The persistence of Cyp creates toxicity to both humans and the environment This study investigates biochar Bacillus cereus distinct collective effects on -contaminated soil during 90-day incubation. also different concentrations (50, 100, ,500 1000 mg kg−1) physicochemical biological activities incubation period. Microbial biomass carbon respiration rates decreased significantly across all cypermethrin concentrations, with most substantial reductions observed at kg−1. However noticeable variations in enzymes MBC over time entire On 1st day, GMean Enz rate treatments ranged from 0.98 0.63, 9.06, 5.03, respectively. Under pollution, microbial exhibited significant decreases, highest inhibition (86.2%) kg−1 day Soil dropped 77%, kg−1, Integrated biomarker response (IBR) values peaked 30, indicating environmental stress. Biochar effectively facilitated degradation Cyp, achieving approximately 85% within first 45 days experiment. combined application increased pH neutral level 5.9, 7.1, reduced electrical conductivity 1.41 µS cm− 1 1.20 cm−1, elevated cation exchange capacity 1.54 ± 0.04 6.18 C mol while improving organic content 3.135%. However, dehydrogenase activity was decresed upto 47% other including urasese catlayse phostasese Gmean enzymeatic were improved. These findings suggest bacterial interaction management enhance resilience against pesticide

Language: Английский

Citations

Dual-domain superoxide dismutase: In silico prediction directed combinatorial mutation for enhanced robustness and catalytic efficiency DOI

Jingjing Liu, Lei Yao, Fang Zhao

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 292, P. 139179 - 139179

Published: Dec. 26, 2024

Language: Английский

Citations