Enhanced Fermentation Process for Production of High Docosahexaenoic Acid Content by Schizochytrium sp. GCD2032 DOI Creative Commons

Liucheng Long,

Xiaoqing Ren,

Feiyu Zhang

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(9), P. 460 - 460

Published: Sept. 4, 2024

There is significant demand for high-purity DHA in the pharmaceutical industry. Traditionally, this extracted from raw materials with relatively low content (10–20%), such as fish oil. Recently, through electroporation-induced mutation, a high-DHA-content strain of Schizochytrium sp. GCD2032 was isolated. To further enhance its production, optimizations were conducted on culture medium and fermentation conditions (in shaking flasks), well different nitrogen source concentrations 5 L fermenter) biomass, fatty acid content, (as percentage total acids). In fermenter, achieved biomass 50 g/L, 55.71% 61.29%. Notably, reached an impressive 341.45 mg/g dry weight. This consistently produces high levels acids DHA, demonstrating potential applications.

Language: Английский

Regulatory Mechanisms of EPA and DHA Proportions in a PUFA-Producing Microalga, Schizochytrium sp. ATCC 20888: From the Biosynthesis and Storage Distribution Aspects DOI
Yaqi Xu, Zhao Zhang,

Yanqing Bian

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Schizochytrium sp. ATCC 20888 is an important species for industrial polyunsaturated fatty acids (PUFA) production. This study investigated the regulatory mechanisms affecting proportions of eicosapentaenoic acid (EPA) and docosahexaenoic (DHA) in terms biosynthesis storage distribution. EPA DHA possessed different accumulation patterns: proportion increased over time, while peaked at 48 h. was predominantly integrated into triacylglycerol during logarithmic phase phosphatidylcholine stationary phase. Transcriptome analysis revealed that synthesis involved synthase-elongase/desaturase system, depended mainly on PUFA synthase. Key enzymes, including elongase ELOVL7, diacylglycerol acyltransferase (g10562), lysophosphatidylcholine acyltransferases (g8836 g7540), show a positive correlation with yield, highlighting their roles its storage. Additionally, phosphopantetheine adenylyl transferase (PPAT/COASY) ADP-ribosylation factor 1_2 (ARF1_2) were identified as potential regulators proportions. provided insights genetic optimization production inSchizochytrium.

Language: Английский

Citations

1
Coupling ultrasound and low temperature stress for enhancing Docosahexaenoic acid production in Schizochytrium sp. DOI

Chun-Xiao Yan,

Shuai Zhang,

Kai-Nan Zhang

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: 64, P. 105854 - 105854

Published: Jan. 7, 2025

Language: Английский

Citations

0
Characterization and Application of Schizochytrium sp. HX-308 Promoters for Enhanced Docosahexaenoic Acid Biosynthesis DOI

Chun-Xiao Yan,

Yi Zheng, Shuai Zhang

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 105948 - 105948

Published: Jan. 1, 2025

Language: Английский

Citations

0
Integrated production of xylose and docosahexaenoic acid from hemicellulose and cellulose in corncob DOI
Xinran Zhang, Jingwen Liu, Xin Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134176 - 134176

Published: Aug. 2, 2024

Language: Английский

Citations

3
Metabolic regulation strategies for enhancing microbial docosahexaenoic acid production by Schizochytrium sp. DOI
Jingwen Liu, Feng Xue, Shang Wang

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(5)

Published: April 28, 2025

Language: Английский

Citations

0
Enhanced Fermentation Process for Production of High Docosahexaenoic Acid Content by Schizochytrium sp. GCD2032 DOI Creative Commons

Liucheng Long,

Xiaoqing Ren,

Feiyu Zhang

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(9), P. 460 - 460

Published: Sept. 4, 2024

There is significant demand for high-purity DHA in the pharmaceutical industry. Traditionally, this extracted from raw materials with relatively low content (10–20%), such as fish oil. Recently, through electroporation-induced mutation, a high-DHA-content strain of Schizochytrium sp. GCD2032 was isolated. To further enhance its production, optimizations were conducted on culture medium and fermentation conditions (in shaking flasks), well different nitrogen source concentrations 5 L fermenter) biomass, fatty acid content, (as percentage total acids). In fermenter, achieved biomass 50 g/L, 55.71% 61.29%. Notably, reached an impressive 341.45 mg/g dry weight. This consistently produces high levels acids DHA, demonstrating potential applications.

Language: Английский

Citations

0