Acetate metabolism during xylose fermentation enhances 3-hydroxypropionic acid production in engineered acid-tolerantIssatchenkia orientalis DOI
Deokyeol Jeong, Da Hye Lee, Junli Liu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Abstract Efficient bioconversion of acetate-rich lignocellulosic biomass into value-added chemicals remains a major challenge due to the toxicity acetic acid. In this study, we engineered an acid-tolerant Issatchenkia orientalis strain (IoDY01H) capable producing 3-hydroxypropionic acid (3-HP), key bioplastic precursor, from glucose, xylose, and acetate. Using Cas9-based genome editing system with hygromycin B resistance marker, introduced heterologous genes encoding xylose utilization β-alanine-based 3-HP biosynthetic pathways I. genome. Metabolomic analysis revealed that acetate supplementation redirected metabolic flux toward amino lipid metabolism while reducing TCA cycle intermediates. Acetate enhanced production by promoting accumulation β-alanine, but also β-alanine–pyruvate aminotransferase as bottleneck under acidic conditions. pretreated hemp stalk hydrolysate feedstock, achieved titer 8.7 g/L via separate hydrolysis fermentation (SHF), outperforming simultaneous saccharification (SSF). These findings demonstrate feasibility using non-conventional yeast highlight promising microbial chassis for industrial bioconversion. Graphical abstract Highlights Engineered co-utilized produce 3-HP. addition during fermentation. biosynthesis pathways. SHF.

Language: Английский

Non-conventional yeasts: promising cell factories for organic acid bioproduction DOI
Yupeng Wang, Yi Wang, Jie Cui

et al.

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
Issatchenkia orientalis as a platform organism for cost-effective production of organic acids DOI Creative Commons
Shih‐I Tan, Zijun Liu, Vinh Tran

et al.

Metabolic Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

1
Native and Recombinant Yeast Producers of Lactic Acid: Characteristics and Perspectives DOI Open Access

А. В. Царук,

Kamila Filip, Andriy А. Sibirny

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2007 - 2007

Published: Feb. 25, 2025

Lactic acid (LA) is a key chemical used in various industries, including food, pharmaceuticals, and bioplastics. Although traditionally produced using lactic bacteria, yeasts offer significant advantages, such as higher tolerance to acidic environments, broader substrate range, the potential for genetic metabolic engineering. This review explores use of Lachancea thermotolerans, Saccharomyces cerevisiae, Kluyveromyces marxianus, lactis, Candida utilis, Pichia kudriavzevii LA producers, highlighting their unique characteristics industrial applications. S. cerevisiae stands out due its robust toolkit tolerance, while K. marxianus offers thermotolerance efficient utilization lactose pentoses, making it ideal high-temperature fermentations. lactis particularly suited valorizing dairy by-products like whey, P. exhibits high multiple stresses, C. utilis demonstrates superior resilience lignocellulosic inhibitors, enabling biorefineries. Key challenges, enhancing optimizing pathways, are addressed through strategies heterologous lactate dehydrogenase (LDH) expression, redox balance modification, adaptive laboratory evolution. The also discusses applications, context circular economy approaches, where can convert waste streams into high-value LA. Future research should focus on integrating scalable, sustainable bioprocesses meet growing demand renewable biodegradable materials.

Language: Английский

Citations

0
Acetate metabolism during xylose fermentation enhances 3-hydroxypropionic acid production in engineered acid-tolerantIssatchenkia orientalis DOI
Deokyeol Jeong, Da Hye Lee, Junli Liu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Abstract Efficient bioconversion of acetate-rich lignocellulosic biomass into value-added chemicals remains a major challenge due to the toxicity acetic acid. In this study, we engineered an acid-tolerant Issatchenkia orientalis strain (IoDY01H) capable producing 3-hydroxypropionic acid (3-HP), key bioplastic precursor, from glucose, xylose, and acetate. Using Cas9-based genome editing system with hygromycin B resistance marker, introduced heterologous genes encoding xylose utilization β-alanine-based 3-HP biosynthetic pathways I. genome. Metabolomic analysis revealed that acetate supplementation redirected metabolic flux toward amino lipid metabolism while reducing TCA cycle intermediates. Acetate enhanced production by promoting accumulation β-alanine, but also β-alanine–pyruvate aminotransferase as bottleneck under acidic conditions. pretreated hemp stalk hydrolysate feedstock, achieved titer 8.7 g/L via separate hydrolysis fermentation (SHF), outperforming simultaneous saccharification (SSF). These findings demonstrate feasibility using non-conventional yeast highlight promising microbial chassis for industrial bioconversion. Graphical abstract Highlights Engineered co-utilized produce 3-HP. addition during fermentation. biosynthesis pathways. SHF.

Language: Английский

Citations

0