Metabolic reprogramming and machine learning-guided cofactor engineering to boost nicotinamide mononucleotide production in Escherichia coli

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132350 - 132350

Published: March 1, 2025

Language: Английский

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High-Temperature Catalytic Platform Powered by Thermophilic Microorganisms and Thermozymes

Synthetic biology and engineering, Journal Year: 2025, Volume and Issue: 3(1), P. 10001 - 10001

Published: Jan. 1, 2025

Language: Английский

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Overproduction of Cucurbitadienol through Modular Metabolic Engineering and Fermentation Optimization in Saccharomyces cerevisiae

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Cucurbitadienol is a key intermediate in the biosynthesis of cucurbitane-type compounds and serves as precursor for mogrosides, cucurbitacins, other valuable natural products potential biological food importance. However, microbial fermentation cucurbitadienol production remains inefficient, limiting its further industrial application. This study achieved efficient synthesis through multimodular strategy. First, an N-degron tag was used to direct metabolic flux toward without compromising cell growth. Second, enzyme engineering strategies were employed improve utilization efficiency metabolites. Finally, increase availability, transcription factor UPC2–1 introduced, which upregulated expression ERGs pre-squalene pathway. After eliminating nitrogen supplementation optimizing conditions, accumulation 5 L bioreactor increased 6.1 g/L, representing highest titer reported date. These findings provide solid foundation industrial-scale derivatives.

Language: Английский

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Adaptive laboratory evolution of Thermoanaerobacterium aotearoense for enhanced ethanol production from raw cassava starch

Renewable Energy, Journal Year: 2024, Volume and Issue: 233, P. 121179 - 121179

Published: Aug. 14, 2024

Language: Английский

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