Genome-Wide Identification and Characterization of Ammonium Transporter (AMT) Genes in Chlamydomonas reinhardtii DOI Open Access
Wenhui Hu, Dan Wang, Shuangshuang Zhao

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(8), P. 1002 - 1002

Published: July 31, 2024

Ammonium transporters (AMTs) are vital plasma membrane proteins facilitating NH4+ uptake and transport, crucial for plant growth. The identification of favorable AMT genes is the main goal improving ammonium-tolerant algas. However, there have been no reports on systematic expression analysis Chlamydomonas reinhardtii (C. reinhardtii) genes. This study comprehensively identified eight CrAMT genes, distributed across chromosomes, all containing more than 10 transmembrane structures. Phylogenetic revealed that CrAMTs belonged to AMT1 subfamily. conserved motifs domains were similar those members OsAMTs AtAMTs. Notably, gene fragments longer contain introns compared AtAMTs OsAMTs. And promoter regions enriched with cis-elements associated hormones light response. Under treatment, CrAMT1;1 CrAMT1;3 significantly upregulated, while CrAMT1;2, CrAMT1;4, CrAMT1;6 saw a notable decrease. CrAMT1;7 CrAMT1;8 also experienced decline, albeit less pronounced. Transgenic algas overexpressed did not show significant difference in growth CC-125, transgenic knockdown exhibited inhibition. or knocked-down displayed reduced which resulted suppression other None showed better CC-125 at high ammonium levels. In summary, our has unveiled potential role high-ammonium environments can serve as foundational research platform investigating algal species.

Language: Английский

Genome-Wide Identification and Characterization of Ammonium Transporter (AMT) Genes in Chlamydomonas reinhardtii DOI Open Access
Wenhui Hu, Dan Wang, Shuangshuang Zhao

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(8), P. 1002 - 1002

Published: July 31, 2024

Ammonium transporters (AMTs) are vital plasma membrane proteins facilitating NH4+ uptake and transport, crucial for plant growth. The identification of favorable AMT genes is the main goal improving ammonium-tolerant algas. However, there have been no reports on systematic expression analysis Chlamydomonas reinhardtii (C. reinhardtii) genes. This study comprehensively identified eight CrAMT genes, distributed across chromosomes, all containing more than 10 transmembrane structures. Phylogenetic revealed that CrAMTs belonged to AMT1 subfamily. conserved motifs domains were similar those members OsAMTs AtAMTs. Notably, gene fragments longer contain introns compared AtAMTs OsAMTs. And promoter regions enriched with cis-elements associated hormones light response. Under treatment, CrAMT1;1 CrAMT1;3 significantly upregulated, while CrAMT1;2, CrAMT1;4, CrAMT1;6 saw a notable decrease. CrAMT1;7 CrAMT1;8 also experienced decline, albeit less pronounced. Transgenic algas overexpressed did not show significant difference in growth CC-125, transgenic knockdown exhibited inhibition. or knocked-down displayed reduced which resulted suppression other None showed better CC-125 at high ammonium levels. In summary, our has unveiled potential role high-ammonium environments can serve as foundational research platform investigating algal species.

Language: Английский

Citations

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