Transcriptome analysis and reverse engineering verification of SNZ3Val125Ile and Pho3Asn134Asp revealed the mechanism of adaptive laboratory evolution to increase the yield of tyrosol in Saccharomyces cerevisiae strain S26-AE2

Biotechnology for Biofuels and Bioproducts, Journal Year: 2025, Volume and Issue: 18(1)

Published: March 5, 2025

Tyrosol is an important drug precursor, and Saccharomyces cerevisiae one of the main microorganisms that produces tyrosol. Although excessive metabolic modification increases production tyrosol, it also causes a decrease in growth rate yeast. Therefore, this study attempted to restore S. through adaptive evolution further improve tyrosol production. After laboratory S26, three evolutionary strains were obtained. The biomass strain S26-AE2 reached 17.82 g DCW/L presence 100 g/L glucose, which was 15.33% higher than its 817.83 mg/L. transcriptome analysis revealed that, upon exposure may reduce transcriptional regulation glucose repression decreased HXK2 expression. expression genes related pyruvate synthesis increased S26-AE2. Meanwhile, levels most tricarboxylic acid cycle-related when cultured with 20 glucose. Furthermore, amount produced by S26 SNZ3Val125Ile mutation 17.01% compared control following In study, strain, S26-AE2, good performance obtained evolution. differences involved pathways be yeast Further reverse engineering verified SNZ3 promoted glucose-rich medium. This provides theoretical basis for synthesise derivatives.

Language: Английский

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Improving an Alternative Glycerol Catabolism Pathway in Yarrowia lipolytica to Enhance Erythritol Production

Yeast, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 11, 2024

ABSTRACT Engineering the glycerol‐3‐phosphate pathway could enhance erythritol production by accelerating glycerol uptake. However, little work has been conducted on alternative dihydroxyacetone (DHA) in Yarrowia lipolytica . Herein, this route was identified and characterized Y. metabolomic transcriptomic analysis. Moreover, reaction catalyzed kinase encoded dak2 as rate‐limiting step. By combining NHEJ‐mediated insertion mutagenesis with a push‐and‐pull strategy, strains high‐yield synthesis from were obtained. Screening of library mutants allows identification mutant fourfold increased production. Overexpression DAK2 dehydrogenase GCY3 together gene encoding transketolase transaldolase nonoxidative part pentose phosphate led to strain further productivity titer 53.1 g/L yield 0.56 g/g glycerol, which 8.1‐ 4.2‐fold starting strain.

Language: Английский

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Continuous Cultivation of Yarrowia lipolytica: Potential, Challenges, and Case Studies

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 619 - 619

Published: Dec. 3, 2024

Currently, the yeast Yarrowia lipolytica is regarded as one of most promising producers protein, lipids, polyols, organic acids, and other metabolites. The objective enhancing efficiency target product biosynthesis can be achieved through improvement strains-producers optimization cultivation conditions. present review assesses potential continuous methods (chemostat, turbidostat, pH-auxostat, changestats, etc.) in order to gain insight into impact strains conditions on productivity developed bioprocesses. utilization enables implementation processes under controlled reproducible conditions, thus stabilizing parameters physiological state producer, obtaining homogeneous samples. focuses nitrogen-limited chemostat cultures, which represent commonly employed strategy for investigating biochemical characteristics Y. developing production erythritol, citric acid, recombinant proteins. To date, such an analysis literature has not been conducted context lipolytica.

Language: Английский

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Transcriptome analysis and reverse engineering verification of SNZ3 Val125Ile and Pho3 Asn134Asp revealed the mechanism of laboratory adaptive evolution to increase the yield of tyrosol in Saccharomyces cerevisiae S26

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Background Tyrosol is an important drug precursor, and Saccharomyces cerevisiae one of the main microorganisms producing tyrosol. Although excessive metabolic modification increased production tyrosol, it also caused a decrease in growth rate yeast. Therefore, this study attempted to restore S. through adaptive evolution further improve tyrosol production. Results After laboratory S26, three evolutionary strains were obtained. The biomass strain S26-AE2 reached 17.82 under condition 100 g/L glucose which was 15.33% higher than that its 817.83 mg/L. Transcriptome analysis showed may decreased expression HXK2 reduce transcriptional regulation repression increase gene PGI1 promote utilization glucose. genes related pyruvate synthesis enhanced S26-AE2. Under 20 condition, TCA cycle-related more active. Furthermore, S26 with SNZ3^Val125Ile mutation by 17.01% compared control Conclusions In paper, good performance obtained evolution. transcriptome reveals differences pathways be yeast Further reverse engineering verified SNZ3 promoted glucose-rich medium. This provides theoretical basis for synthesize derivatives.

Language: Английский

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