Enhancing Digestibility and Intestinal Peptide Release of Pleurotus eryngii Protein: An Enzymatic Approach DOI Creative Commons
Dandan Wang, Meng Zhang,

Jianing Wan

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(12), P. 890 - 890

Published: Dec. 23, 2024

Pleurotus eryngii is a tasty and low-calorie mushroom containing abundant high-quality protein. This study aims to improve the digestibility of P. protein (PEP) hence facilitate its development as healthy alternative The extracted PEP was pretreated with 1000–5000 U papain, neutral protease alkaline protease. Chyme collected from in vitro simulated gastrointestinal digestion analyzed by fluorescence microscopy particle analyzer, endpoint profiles peptides amino acids were determined UHPLC-MS/MS NanoLC-MS/MS. size curve images jointly supported that hydrolysis improved decomposition dispersion during digestion, particularly gastric phase. impact on Zeta potential minimal. Proteases effectively increased abundance after L-isomer Lys Arg Maximum release achieved when 5000 protease, reaching 7.54 times control. Pretreatments proteases also notably digestive yields 16,736–19,870 peptides, maximum 1.70 control, which mainly consisted small composed 7–15 molecular weight below 800 Da. findings indicated hydrolysis, especially pretreatment enhanced PEP, shed light providing enzymatic approaches for improving bioavailability developing fungal proteins.

Language: Английский

Sustainable production of plant biostimulants from cephalosporin fermentation residues: ultrasonic dissolution and enzymatic hydrolysis DOI
Dongze Niu, P. Ding,

Wenying An

et al.

Biochemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 109658 - 109658

Published: Jan. 1, 2025

Language: Английский

Citations

0
Synergy of Multi-Enzyme Pretreatment and Paraclostridium benzoelyticum Bioaugmentation: A Dual Strategy for Enhancing Methane Production in Dry Anaerobic Digestion of Kitchen Waste DOI
Xiaoming Sun,

Panpan Hu,

Mengyao Xiao

et al.

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121671 - 121671

Published: April 1, 2025

Language: Английский

Citations

0
Effects of different types of municipal solid waste incineration slag on landfill stabilization and related microbiological mechanism DOI
Yanan Wang, Yahui Li, Wenyu Yang

et al.

Waste Management, Journal Year: 2025, Volume and Issue: 202, P. 114833 - 114833

Published: April 26, 2025

Language: Английский

Citations

0
Exploring Optimal Pretreatment Approaches for Enhancing Biohydrogen and Biochar Production from Azolla filiculoides Biomass DOI Open Access
Mohamed El-Qelish, Saber A. El‐Shafai, Mohamed Mahmoud

et al.

Water, Journal Year: 2024, Volume and Issue: 16(21), P. 3048 - 3048

Published: Oct. 24, 2024

Mitigating the irreversible consequences of climate change necessitates application sustainable energy resources. Hereby, we investigated biological anaerobic fermentation Azolla filiculoides biomass for biohydrogen production as a clean renewable source. is widely growing aquatic plant in polluted freshwater streams. However, high non-biodegradable organic matter content remains challenging efficiently producing energy, especially when it being used sole donor substrate. In order to overcome this challenge, different pretreatment scenarios (namely, alkali, autoclaving, and ultrasonication) have been employed enhancing hydrolysis maximize and, consequently, potential. The potential was 250.5, 398, 414.5, 439.5 mL-H2, giving hydrogen yield 60.1, 89.6, 92.9, 107.9 mL-H2/g-VS, respectively. Gompertz kinetics were applied estimate growth parameters process, which revealed good fit with R2 ranging from 0.96 0.98. produced digestate valorized biochar production, material that could be water treatment purposes. characterized using physical analyses, including FTIR, SEM, EDX, TEM. physicochemical characterizations demonstrate successful formation highly porous structure rough surface, evidenced by emergence significant functional groups (e.g., O-H, C-H, C=C, C=O) existing on surface biochar. conclusion, study harnesses approach waste streams, represents circular economy model transforming materials into valuable products reducing reliance non-renewable

Language: Английский

Citations

1
Enhancing Digestibility and Intestinal Peptide Release of Pleurotus eryngii Protein: An Enzymatic Approach DOI Creative Commons
Dandan Wang, Meng Zhang,

Jianing Wan

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(12), P. 890 - 890

Published: Dec. 23, 2024

Pleurotus eryngii is a tasty and low-calorie mushroom containing abundant high-quality protein. This study aims to improve the digestibility of P. protein (PEP) hence facilitate its development as healthy alternative The extracted PEP was pretreated with 1000–5000 U papain, neutral protease alkaline protease. Chyme collected from in vitro simulated gastrointestinal digestion analyzed by fluorescence microscopy particle analyzer, endpoint profiles peptides amino acids were determined UHPLC-MS/MS NanoLC-MS/MS. size curve images jointly supported that hydrolysis improved decomposition dispersion during digestion, particularly gastric phase. impact on Zeta potential minimal. Proteases effectively increased abundance after L-isomer Lys Arg Maximum release achieved when 5000 protease, reaching 7.54 times control. Pretreatments proteases also notably digestive yields 16,736–19,870 peptides, maximum 1.70 control, which mainly consisted small composed 7–15 molecular weight below 800 Da. findings indicated hydrolysis, especially pretreatment enhanced PEP, shed light providing enzymatic approaches for improving bioavailability developing fungal proteins.

Language: Английский

Citations

0