Dissimilatory nitrate reduction to ammonium driven by different electron donors: Mechanisms, recent advances, and future perspectives

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160625 - 160625

Published: Feb. 1, 2025

Language: Английский

Insights into the Direct Photoelectron Transfer Mechanism in Cofactor-free Redox Carbon Dots and Cytochrome c Nitrite Reductase Biohybrids Responsible for Ammonia Synthesis

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Highly efficient NH3 production has been reported to be achieved by photosensitizing Shewanella oneidensis MR-1 using carbon dots (CDs). During this process, cytochrome c nitrite reductase (NrfA) is regarded as the rate-limiting enzyme. However, precise electron transfer mechanism between CDs and NrfA remains unclear. Herein, a hybrid photosynthetic system composed of redox (NrfA/R-CDs) was constructed, achieving maximum rate 12.5 ± 1.1 μmol (NH3)·mg–1 (NrfA)·h–1. R-CDs with aromatic ketone groups could store photoinduced electrons, enhancing carrier separation efficiency. These stored photoelectrons were capable being directly transferred without need cofactors. Even under dark conditions, direct occurred from in NrfA, providing an indirect illumination approach reduce phototoxicity NrfA. Molecular docking dynamics simulations demonstrated formation stable complex heme 2 region R-CDs. The short distance (10 Å) π-electronic interactions facilitated electronic process. This study provides comprehensive understanding interfacial photoelectron guidelines for constructing nanomaterials storage release properties.

Language: Английский

Accelerating mechanism of carbon dots-modified biocarrier on anammox process using metagenomic and metatranscriptomic techniques

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 163051 - 163051

Published: April 1, 2025

Language: Английский