Structural characterization and antioxidant activity of a polysaccharide produced by Chaetomium globosum CGMCC 6882 from waste tobacco leaves

Preparative Biochemistry & Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 10

Published: Feb. 18, 2025

Due to the restricts of treatment methods and high costs, waste tobacco materials are usually discarded, which leads resources environmental pollution. Present work used leaves as carbon source produce polysaccharide (CGP-TL) by Chaetomium globosum CGMCC 6882 via submerged fermentation. CGP-TL yield was 1.62 ± 0.35 g/L, contents carbohydrate protein in were 91.73% 6.49% 5.51% 0.84%. Structural characterization showed composed rhamnose, arabinose, galactose, glucose, mannose xylose a molar ratio 9.9688: 6.8482: 19.6045: 29.4448: 0.8033: 0.5874. Meanwhile, its weight-average molecular weight, number-average weight polydispersity 286.035 kDa, 118.146 kDa 2.421, respectively. In vitro antioxidant analysis scavenged DPPH, ABTS, hydroxyl superoxide radicals positively correlated concentration, corresponding scavenging effects at 2.5 mg/mL 75.53% 1.89%, 96.24% 2.32%, 52.91% 2.28% 65.26% 2.47%. Furthermore, not only improved antioxidative status RAW 264.7 cells, but also exerted oxidative repairing on H2O2-damaged cells increasing levels SOD, CAT GSH-Px, reducing MDA level. Overall, this will provide references for green efficient utilization cellulose resources.

Language: Английский

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Genome Mining of the Biocontrol Agent Trichoderma afroharzianum Unearths a Key Gene in the Biosynthesis of Anti-Fungal Volatile Sesquiterpenoids

Catalysts, Journal Year: 2025, Volume and Issue: 15(4), P. 341 - 341

Published: April 1, 2025

The volatile organic compounds (VOCs) in Trichoderma afroharzianum ACCC 33109 have the biological activities of both hydrolytic enzymes and antimicrobial peptides to mitigate attack by phytopathogens spread over long distances soil. However, biosynthesis pathway anti-fungal VOCs has not been elucidated yet. In this study, we identified 15 genes (TaTS1–15) coding for putative terpene synthase with low identities (<79.54%) functionally characterized homologs through genome mining. Upon Fusarium induction, relative expression levels nine TaTS were up-regulated up 2793-fold (TaTS9). To verify contribution TaTS9 synthesis VOCs, knockout mutant strain was constructed its antagonistic activities, transcript profiles, VOC metabolomes. Heterologous Escherichia coli produced target gene product, which converted precursor farnesyl pyrophosphate (FPP) into β-cubenene (>90%) γ-amorphene. Thus, confirmed as first Trichoderma, catalyzes various sesquiterpenes activities. This study provides insight key sesquiterpenoids potential applications agriculture food industries.

Language: Английский

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Redesigning and rethinking genetic circuits: the potential of transcriptional rewiring in filamentous fungi

Current Opinion in Biotechnology, Journal Year: 2025, Volume and Issue: 93, P. 103301 - 103301

Published: April 4, 2025

Language: Английский

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A Comprehensive Review on Chemical Structures and Bioactivities of Ostropomycetidae Lichens

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(5), P. 369 - 369

Published: May 9, 2025

Lichenized fungi, recognized as an ecologically vital and pharmaceutically promising resource, hold substantial value in both environmental conservation medicinal applications. As the second largest subclass within lichen-forming fungi of Lecanoromycetes, Ostropomycetidae emerged a critical reservoir bioactive secondary metabolites. Current research has revealed that these metabolites demonstrate remarkable bioactivities, positioning them potential sources for novel pharmaceutical compounds. Despite considerable progress characterizing chemical constituents evaluating bioactivities this subclass, systematic summary discoveries remains absent. This review synthesizes lichenochemical progress, providing evaluations 202 structurally characterized compounds from lichen species over recent decades. These Ostropomycetidae-derived cover phenols, polyketides, fatty acids, terpenoids, steroids, non-ribosomal peptides, exhibit diverse including antitumor, anti-inflammatory, antibacterial, antifungal, antiviral, antioxidant, anti-angiogenic, anti-neurodegenerative diseases, antitubercular, anti-herbivore, antitrypanosomal, so on. The aim is to establish robust chemodiversity framework offer strategic guidance targeted exploration lichen-derived drug candidates biological resources lichens.

Language: Английский

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