Enhanced Biosynthesis of 6’-Sialyllactose in Escherichia coli via Systematic Metabolic Engineering DOI
Yue Song, Nan‐Kai Wang, Jin‐Ping Chen

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

6'-Sialyllactose (6'-SL), a sialylated oligosaccharide belonging to human milk oligosaccharides (HMOs), finds extensive applications in food, pharmaceuticals, cosmetics, and particularly infant nutrition dietary supplements. In this study, Escherichia coli K12 MG1655 was subjected metabolic engineering enhance 6'-SL biosynthesis. Initial pathway optimization involved the deletion of competing genes (nanATEK, nagAB, lacZ). Following overexpression NmCSS Pd2,6ST, an initial titer 0.98 g/L achieved. Subsequent combinatorial expression heterologous enzymes from diverse microbial sources increased yield 1.26 g/L. Through systematic promoter strength ribosome binding site (RBS) elements, further elevated 2.41 Implementation structure-guided mutagenesis on both key resulted significant improvement 4.27 Final strategies included rate-limiting CTP biosynthesis pathway. The engineered strain demonstrated production capacity 4.92 shake-flask cultures. We transferred 5-L fermenter achieved final 12.82 after 76 h cultivation, demonstrating 13-fold enhancement compared culture.

Language: Английский

The Strategy and Application of Gene Attenuation in Metabolic Engineering DOI Creative Commons
Yahui Zhang, Zhaoxia Jin, Linxia Liu

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 927 - 927

Published: April 17, 2025

Metabolic engineering has a wide range of applications, spanning key sectors such as energy, pharmaceuticals, agriculture, chemicals, and environmental sustainability. Its core focus is on precisely modulating metabolic pathways to achieve efficient, sustainable, environmentally friendly biomanufacturing processes, offering new possibilities for societal sustainable development. Gene attenuation critical technique within engineering, pivotal in optimizing fluxes improving target metabolite yields. This review article discusses gene mechanisms, the applications across various biological systems, implementation strategies. Additionally, we address potential future challenges explore its drive further advancements field.

Language: Английский

Citations

0
Intensification of 2′-Fucosyllactose biosynthesis pathway by using a novel fucosyltransferase from Bacillus cereus DOI Creative Commons

Kainuo Zhang,

Miaomiao Gao, Chang Cao

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 30, 2025

2'-Fucosyllactose (2'-FL) is an oligosaccharide that can be synthesized in the human body and known for its health-promoting prebiotic effects. The biosynthesis of 2'-FL using microorganisms has received attention recently due to increased application nutritional medical infant formulations. This work attempts new Bacillus cereus α-1,2-fucosyltransferase (FutCB) de novo synthesis Escherichia coli (E. coli). Additionally, knocking out LacZ WaaF genes alongside overexpression key gmd, manB, wcaG, manC enhances availability necessary precursors GDP-L-fucose lactose 2'-FL. use constitutive promoters achieved better control over production during fed-batch fermentation. After 64 h fermentation, modified E. strains produced 121.4 g/L with a yield 1.90 g/L/h, resulting impressive output. These results together indicate potential large-scale, high-yield form basis much more refinement done. next step will focus on maximum substrate utilization, alteration gene regulation, improvement commercial-scale synthesis.

Language: Английский

Citations

0
Enhanced Biosynthesis of 6’-Sialyllactose in Escherichia coli via Systematic Metabolic Engineering DOI
Yue Song, Nan‐Kai Wang, Jin‐Ping Chen

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

6'-Sialyllactose (6'-SL), a sialylated oligosaccharide belonging to human milk oligosaccharides (HMOs), finds extensive applications in food, pharmaceuticals, cosmetics, and particularly infant nutrition dietary supplements. In this study, Escherichia coli K12 MG1655 was subjected metabolic engineering enhance 6'-SL biosynthesis. Initial pathway optimization involved the deletion of competing genes (nanATEK, nagAB, lacZ). Following overexpression NmCSS Pd2,6ST, an initial titer 0.98 g/L achieved. Subsequent combinatorial expression heterologous enzymes from diverse microbial sources increased yield 1.26 g/L. Through systematic promoter strength ribosome binding site (RBS) elements, further elevated 2.41 Implementation structure-guided mutagenesis on both key resulted significant improvement 4.27 Final strategies included rate-limiting CTP biosynthesis pathway. The engineered strain demonstrated production capacity 4.92 shake-flask cultures. We transferred 5-L fermenter achieved final 12.82 after 76 h cultivation, demonstrating 13-fold enhancement compared culture.

Language: Английский

Citations

0