
Environment International, Journal Year: 2025, Volume and Issue: 199, P. 109510 - 109510
Published: May 1, 2025
The transfer of antibiotic resistance genes (ARGs) from fertilized soil to vegetables, particularly those consumed raw, causes significant public health risks through the food chain. Black soldier fly larvae can efficiently convert animal manure into organic fertilizer with reduced resistance. This study utilized metagenomic sequencing investigate fields treated control (COF), black (BOF), and no fertilizer, aim assessing ARGs cherry radish. results indicated that BOF significantly richness abundance in both radish compared COF, reducing 13 ARG subtypes a 27.6% decrease Moreover, positive correlation was observed between mobile genetic elements (MGEs) virulence factors (VFs) ARGs, treatment resulting relative reduction 32.8% 29.1%, respectively. complexity networks involving MGEs, VFs, microbial communities 54.2%, 32.3%, lower, respectively, than COF treatment. Further analysis metagenomic-assembled genomes (MAGs) revealed co-occurrence VFs radish, indicating presence potential pathogenic antibiotic-resistant bacteria (PARB). Notably, these PARB radishes decreased by 45.6% COF. These findings underscore efficacy insect mitigating highlighting significance sustainable agricultural practices managing environmental associated ARGs.
Language: Английский