A novel Recjf Exo signal amplification strategy based on bioinformatics-assisted truncated aptamer for efficient fluorescence detection of AFB1 DOI
Yiting Fan,

Khalid Amin,

Wendan Jing

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 254, P. 128061 - 128061

Published: Nov. 12, 2023

Language: Английский

DNA framework signal amplification platform-based high-throughput systemic immune monitoring DOI Creative Commons
Ye Chen, Xingyu Chen, Bowen Zhang

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Feb. 7, 2024

Abstract Systemic immune monitoring is a crucial clinical tool for disease early diagnosis, prognosis and treatment planning by quantitative analysis of cells. However, conventional using flow cytometry faces huge challenges in large-scale sample testing, especially mass health screenings, because time-consuming, technical-sensitive high-cost features. the lack high-performance detection platforms hinders development high-throughput technology. To address this bottleneck, we constructed generally applicable DNA framework signal amplification platform (DSAP) based on post-systematic evolution ligands exponential enrichment tetrahedral framework-structured probe design to achieve high-sensitive diverse cells, including CD4+, CD8+ T-lymphocytes, monocytes (down 1/100 μl). Based advanced platform, present novel immune-cell phenotyping system, DSAP, achieving 30-min one-step without cell washing subset showing comparable accuracy with while significantly reducing time cost. As proof-of-concept, DSAP demonstrates excellent diagnostic immunodeficiency staging 107 HIV patients (AUC > 0.97) within 30 min, which can be applied infection screening. Therefore, initially introduced promising open robust routes point-of-care device development.

Language: Английский

Citations

49
Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review DOI Creative Commons
Yanru Wang, Cui Zhang, Jianlong Wang

et al.

Toxins, Journal Year: 2022, Volume and Issue: 14(2), P. 73 - 73

Published: Jan. 20, 2022

Mycotoxins are secondary metabolites produced by fungal species, which pose significant risk to humans and livestock. The mycotoxins from

Language: Английский

Citations

43
A novel method for detection of ochratoxin A in foods—Co-MOFs based dual signal ratiometric electrochemical aptamer sensor coupled with DNA walker DOI

Yan Guan,

Peng-Bin Si,

Tong Yang

et al.

Food Chemistry, Journal Year: 2022, Volume and Issue: 403, P. 134316 - 134316

Published: Sept. 19, 2022

Language: Английский

Citations

42
Carbon dot- and gold nanocluster-based three-channel fluorescence array sensor: Visual detection of multiple metal ions in complex samples DOI
Shuo Wang, Gaoqiong Deng, Jian Yang

et al.

Sensors and Actuators B Chemical, Journal Year: 2022, Volume and Issue: 369, P. 132194 - 132194

Published: June 11, 2022

Language: Английский

Citations

39
Dual-signal electrochemical aptasensor involving hybridization chain reaction amplification for aflatoxin B1 detection DOI
Chen Liu, Ting Wu, Wei Zeng

et al.

Sensors and Actuators B Chemical, Journal Year: 2022, Volume and Issue: 371, P. 132494 - 132494

Published: Aug. 8, 2022

Language: Английский

Citations

39
DNAzyme-driven tripedal DNA walker for ratiometric electrochemical aptasensor ultrasensitive detection of aflatoxin B1 DOI
Jiong Liang, Yunzhe Zhang,

Zikun Li

et al.

Food Control, Journal Year: 2024, Volume and Issue: 164, P. 110573 - 110573

Published: May 9, 2024

Language: Английский

Citations

8
Signal enhancing strategies in aptasensors for the detection of small molecular contaminants by nanomaterials and nucleic acid amplification DOI
Nan Zhang, Jingrong Li, Boshi Liu

et al.

Talanta, Journal Year: 2021, Volume and Issue: 236, P. 122866 - 122866

Published: Sept. 9, 2021

Language: Английский

Citations

51
Regulation of Ru(bpy)32+ Electrochemiluminescence Based on Distance-Dependent Electron Transfer of Ferrocene for Dual-Signal Readout Detection of Aflatoxin B1 with High Sensitivity DOI
Yuye Li,

Dong Liu,

Shuyun Meng

et al.

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 94(2), P. 1294 - 1301

Published: Dec. 29, 2021

Ferrocene (Fc) is a common quencher of Ru(bpy)32+ luminescence. However, interactions between Fc and can be extremely complicated. In this work, we reported the first use to regulate electrochemiluminescence (ECL) by tuning length DNA sequence luminophore nitrogen-doped graphene quantum dots-Ru(bpy)32+-doped silica nanoparticles (SiO2@Ru-NGQDs). The ECL SiO2@Ru-NGQDs was depressed when distance less than 8 nm; stronger observed more 12 nm. switching attributed electron transfer mechanism, in which participated redox for "signal-off" ECL; favored at electrode fabricated with an Fc-labeled aptamer (Fc-apt) "signal-on" depending on sequence. Here, dual-signal readout aptasensor aflatoxin B1 (AFB1) detection developed via enhanced Fc-apt. currents were simultaneously collected as yardsticks, both decreased higher concentrations AFB1. allowed linear ranges 3 × 10–5 1 102 ng mL–1 mode 10–3 103 electrochemical mode. Our work provides insight into Ru(bpy)32+. strategy potential platform versatile design aptasensors.

Language: Английский

Citations

48
Specific and sensitive detection of CircRNA based on netlike hybridization chain reaction DOI

Jiani Dong,

Zhuoer Zeng,

Ruowei Sun

et al.

Biosensors and Bioelectronics, Journal Year: 2021, Volume and Issue: 192, P. 113508 - 113508

Published: July 16, 2021

Language: Английский

Citations

41
Dual-ratiometric electrochemical aptasensor enabled by programmable dynamic range: Application for threshold-based detection of aflatoxin B1 DOI
Yuye Li, Dong Liu,

Shuyun Meng

et al.

Biosensors and Bioelectronics, Journal Year: 2021, Volume and Issue: 195, P. 113634 - 113634

Published: Sept. 20, 2021

Language: Английский

Citations

41