Post-Vaccination Detection of SARS-CoV-2 Antibody Response with Magnetic Nanoparticle-Based Electrochemical Biosensor System

Biosensors, Journal Year: 2023, Volume and Issue: 13(9), P. 851 - 851

Published: Aug. 26, 2023

Here, we report magnetic nanoparticle-based biosensor platforms for the rapid detection of SARS-CoV-2 antibody responses in human serum. The use proposed system enabled anti-SARS-CoV-2 spike (S) and nucleocapsid (N) proteins at a concentration ng/mL both buffer real serum samples. In particular, protocol, which is considered an indicator innate immunity after vaccination or post-infection, could be useful evaluation response. We included total 48 volunteers who either had COVID-19 but were not vaccinated with CoronoVac Biontech. Briefly, this study, was planned as cohort, samples examined 3, 6, 12 months from time volunteers' showed symptoms respect to response system. Anti-S Ab anti-N detected limit 0.98 0.89 ng/mL, respectively. These data confirmed corresponding commercial electrochemiluminescence immunoassay (ECLIA) assays. Compared ECLIA, more stable obtained, especially collected over 6 months. After period, drop observed. Our findings that it platform exploring dynamics immune response, has translational potential clinic. conclusion, MNP-based together its counterparts previous studies, candidate determining natural post-vaccination well reducing workload medical personnel paving way screening studies on vaccine efficacy.

Language: Английский

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Rational Design of Trident Aptamer Scaffold for Rapid and Accurate Monitoring of 25-Hydroxyvitamin D₃ Metabolism in Living Cells

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(27), P. 10322 - 10329

Published: June 20, 2023

The level of 25-hydroxyvitamin D3 [25(OH)VD3] in human blood is considered as the best indicator vitamin D status, and its deficiency or excess can lead to various health problems. Current methods for monitoring 25(OH)VD3 metabolism living cells have limitations terms sensitivity specificity are often expensive time-consuming. To address these issues, an innovative trident scaffold-assisted aptasensor (TSA) system has been developed online quantitative complex biological environments. Through computer-aided design, TSA includes aptamer molecule recognition layer that uniformly oriented, maximizing binding site availability, enhancing sensitivity. achieved direct, highly sensitive, selective detection over a wide concentration range (17.4–12,800 nM), with limit 17.4 nM. Moreover, we evaluated efficacy biotransformation liver cancer (HepG2) normal (L-02), demonstrating potential platform drug–drug interaction studies candidate drug screening.

Language: Английский

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Dynamic Profiling and Prediction of Antibody Response to Booster Inactivated Vaccines by Microsample-driven Biosensor and Machine Learning

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 26, 2024

Knowledge on the antibody response to inactivated vaccines in third dose is crucial because it one of primary global vaccination programs. This study integrated microsampling with optical biosensors profile neutralizing antibodies (NAbs) fifteen vaccinated healthy donors, followed by application machine learning predict at given timepoints. Over a nine-month duration, and venipuncture were conducted seven individual A refined iteration fiber optic-biolayer interferometry (FO-BLI) biosensor was designed, enabling rapid multiplexed biosensing NAbs towards both wild-type Omicron variants minutes. Findings revealed strong correlation (Pearson r 0.919, specificity 100%) between levels microsamples sera. Following dose, Sera for wide-type increased 2.9-fold after days 3.3-fold within month, subsequently waning becoming undetectable three months. Considerable but incomplete escape latest omicron subvariants from booster vaccine elicited confirmed, although higher number binding (BAbs) identified another FO-BLI Significantly, highly correlated pseudovirus neutralization assay identifying capacities 0.983). Additionally, demonstrated exceptional accuracy predicting an error <5% BAbs across multiple Microsample-driven enables individuals access their results hours self-collection, while precise models could guide personalized strategies. The technology's innate adaptability positions its potential effective translation diseases prevention development.

Language: Английский

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Universal protection against SARS-CoV-2 viruses by multivalent mRNA vaccine in mice

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 8, 2023

Abstract The continual emergence of new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants challenges available SARS-CoV-2 vaccines for adequate control outbreaks. Currently, universal capable obviating the need exact strain matching between mRNA and circulating viruses are absent. In this study, we designed, manufactured, evaluated a nucleoside-modified lipid nanoparticle vaccine, aimed offering broad-spectrum protection against recent variants. Additionally, efficiency monovalent, bivalent, quadrivalent, XBB.1.5 was compared with proposed vaccine. neutralizing antibody activity wuhan-1, BA.4/5, XBB.1.5, B.1.1.529, BQ.1.1, EG.5.1 JN.1 assessed using enzyme-linked immunosorbent assay, rapid fiber-optic biolayer interferometry-based biosensor, pseudovirus neutralization test. Our results reveal that multivalent vaccine affords comprehensive previously circulating, current unidentified strains.

Language: Английский

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