Construction of Linear Causal-regulated and Autocatalysis-driven DNA Circuits for Highly Sensitive and Specific Detection of Salmonella Typhimurium DOI
Xinru Ren, Weiqing Sun, Bowen Li

et al.

Food Control, Journal Year: 2025, Volume and Issue: 172, P. 111153 - 111153

Published: Jan. 11, 2025

Language: Английский

A novel fluorescent biosensor for sensitive detection of pathogenic bacteria based on catalytic hairpin assembly and λ-Exonuclease cleavage reaction DOI

Jincheng Han,

Shengjun Bu, Hongyu Zhou

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 406, P. 135433 - 135433

Published: Feb. 2, 2024

Language: Английский

Citations

4
A highly sensitive Lock-Cas12a biosensor for detection and imaging of miRNA-21 in breast cancer cells DOI
Jiawei Peng, Ting Liu,

Liwen Guan

et al.

Talanta, Journal Year: 2024, Volume and Issue: 273, P. 125938 - 125938

Published: March 16, 2024

Language: Английский

Citations

4
Harnessing CRISPR/Cas Systems for DNA and RNA Detection: Principles, Techniques, and Challenges DOI Creative Commons

Heyjin Son

Biosensors, Journal Year: 2024, Volume and Issue: 14(10), P. 460 - 460

Published: Sept. 26, 2024

The emergence of CRISPR/Cas systems has revolutionized the field molecular diagnostics with their high specificity and sensitivity. This review provides a comprehensive overview principles recent advancements in harnessing for detecting DNA RNA. Beginning an exploration mechanisms key Cas proteins underpinning systems, navigates detection both pathogenic non-pathogenic nucleic acids, emphasizing pivotal role CRISPR identifying diverse genetic materials. discussion extends to integration various signal-readout techniques, including fluorescence, electrochemical, colorimetric, as well imaging biosensing methods, highlighting advantages limitations practical applications. Furthermore, critical analysis challenges field, such target amplification, multiplexing, quantitative detection, underscores areas requiring further refinement. Finally, concludes insights into future directions CRISPR-based acid potential these continue driving innovation diagnostics, broad implications research, clinical practice, biotechnology.

Language: Английский

Citations

4
Ultrasensitive detection of microRNAs based on cascade amplification strategy of RCA-PER and Cas12a DOI

Chuanjing Ju,

Xue Li, Dongxia Wang

et al.

The Analyst, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Since microRNAs (miRNAs) serve as markers for early cancer diagnosis, it is crucial to develop a novel biosensor detect miRNAs quickly, sensitively and selectively. Hence, we developed fluorescence based on target miRNA-initiated rolling circle amplification (RCA) generate RCA products with multiple tandem catalytic hairpin DNA templates that trigger primer exchange reactions (PER) which extend short single-strand (ssDNA) primers into long ssDNA. Subsequently, the ssDNA activates trans-cleavage activity of clustered regularly interspaced palindromic repeats (CRISPR)/Cas12a system cleave fluorescent reporter chain, enabling ultrasensitive detection through output signal. The could quantify miRNA-141 concentrations from 100 105 pM, limit 94 fM. Therefore, biosensing strategy proposed in this study offers robust technique clinical diagnosis miRNA-141.

Language: Английский

Citations

0
Construction of Linear Causal-regulated and Autocatalysis-driven DNA Circuits for Highly Sensitive and Specific Detection of Salmonella Typhimurium DOI
Xinru Ren, Weiqing Sun, Bowen Li

et al.

Food Control, Journal Year: 2025, Volume and Issue: 172, P. 111153 - 111153

Published: Jan. 11, 2025

Language: Английский

Citations

0