Cited by CRISPR molecular detection techniques: Advances from single to multiple detection methods

Signal Amplification by the trans-Cleavage Activity of CRISPR-Cas Systems: Kinetics and Performance DOI

Wei Feng, Hongquan Zhang, X. Chris Le

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(1), P. 206 - 217

Published: Jan. 10, 2023

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTSignal Amplification by the trans-Cleavage Activity of CRISPR-Cas Systems: Kinetics and PerformanceWei FengWei FengDivision Analytical Environmental Toxicology, Department Laboratory Medicine Pathology, Faculty Dentistry, University Alberta, 10-102 Clinical Sciences Building, Edmonton, Canada T6G 2G3More Wei FengView Biographyhttps://orcid.org/0000-0003-1081-7914, Hongquan ZhangHongquan ZhangDivision ZhangView Biographyhttps://orcid.org/0000-0003-1088-9862, X. Chris Le*X. LeDivision 2G3*[email protected]More LeView Biographyhttps://orcid.org/0000-0002-7690-6701Cite this: Anal. Chem. 2023, 95, 1, 206–217Publication Date (Web):January 10, 2023Publication History Received16 October 2022Published online10 January 2023Published inissue 10 2023https://doi.org/10.1021/acs.analchem.2c04555Copyright © 2023 The Authors. Published American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoiceCC: Creative CommonsBY: Credit must be given to creatorNC: Only noncommercial uses work are permittedND: No derivatives or adaptations permittedArticle Views712Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence for article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InReddit (8 MB) Get e-AlertsSUBJECTS:Crystal cleavage,Genetics,Kinetics,Nucleic acids,Peptides proteins e-Alerts

Language: Английский

Citations

Argonaute-triggered visual and rebuilding-free foodborne pathogenic bacteria detection DOI

Yaru Li,

Jun Kou,

Han Xiao

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 454, P. 131485 - 131485

Published: April 25, 2023

Language: Английский

Citations

Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction DOI

Yanmei Zhou,

Shupu Xie,

Bo Liu

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(6), P. 3332 - 3339

Published: Jan. 30, 2023

Herein, a chemiluminescence (CL) biosensor based on CRISPR-Cas12a and cation exchange reaction was constructed to detect the biomarker microRNA-21 (miRNA-21). The rolling circle amplification (RCA) introduced convert each target RNA strand into long single-stranded DNA with repeated sequences, which acted as triggers initiate transcleavage activity of CRISPR-Cas12a. activated Cas12a could cleave biotinylated linker CuS nanoparticles (NPs) inhibit binding NPs streptavidin immobilized surface microplate, strongly reduced generation Cu2+ from between AgNO3, thus efficiently suppressed CL Cu2+-luminol-H2O2 system, giving "signal off" biosensor. With multiple amplification, detection limit developed sensor for miRNA-21 reached 16 aM. In addition, this is not only suitable professional instrument but also smartphone used tool purpose portable low-cost assay. This method be specifically quite low level in human serum samples various cancer cells, indicating its potential ultrasensitive molecular diagnostics.

Language: Английский

Citations

Functional DNA sensors integrated with nucleic acid signal amplification strategies for non-nucleic acid targets detection DOI

Yuanyuan Zhu,

Jian Wu, Qingli Zhou

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 230, P. 115282 - 115282

Published: April 1, 2023

Language: Английский

Citations

Smartphone-assisted self-powered dual-mode biosensor designed on binary 3D DNA Walkers mediated CRISPR/Cas12a system DOI

Jinyue Shi,

Peiyuan Li, Yumei Huang

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 483, P. 149231 - 149231

Published: Jan. 30, 2024

Language: Английский

Citations

CRISPR/Cas-based nucleic acid detection strategies: Trends and challenges DOI

Jian Zhou, Zhuo Li, Joshua Seun Olajide

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(4), P. e26179 - e26179

Published: Feb. 1, 2024

CRISPR/Cas systems have become integral parts of nucleic acid detection apparatus and biosensors. Various such as CRISPR/Cas9, CRISPR/Cas12, CRISPR/Cas13, CRISPR/Cas14 CRISPR/Cas3 utilize different mechanisms to detect or differentiate biological activities nucleotide sequences. Usually, CRISPR/Cas-based are combined with polymerase chain reaction, loop-mediated isothermal amplification, recombinase amplification transcriptional technologies for effective diagnostics. Premised on these, many biosensors been developed acids viral bacterial pathogens in clinical samples, well other applications life sciences including biosecurity, food safety environmental assessment. Additionally, showed better specificity compared molecular diagnostic methods. In this review, we give an overview various methods highlight some advances their development components. We also discourse operational challenges advantages disadvantages systems. Finally, important considerations offered the improvement testing.

Language: Английский

Citations

CRISPR/Cas12a-based biosensors for environmental monitoring and diagnostics DOI

Atif Khurshid Wani, Nahid Akhtar, Tahir ul Gani Mir

et al.

Environmental Technology & Innovation, Journal Year: 2024, Volume and Issue: 34, P. 103625 - 103625

Published: April 4, 2024

Contaminants, such as nucleic acids or toxic small molecules, threaten both human health and ecosystems when they infiltrate the environment. The precise highly sensitive identification of contaminants holds paramount importance across diverse domains, including safeguarding food integrity, facilitating clinical diagnostics, monitoring environmental conditions. Traditional methodologies, encompassing spectroscopy, chromatography, sequencing, metagenomics, have conventionally served pivotal roles in detection processes. Nevertheless, these methods encountered recurring challenges related to sensitivity, specificity, portability. This review focuses on groundbreaking CRISPR/Cas12-based biosensors. These biosensors leverage incredible precision programmability CRISPR/Cas system recognize specific targets. Here, we comprehensively assess fundamental mechanisms that enable detection, ranging from guide RNA design collateral cleavage. versatility CRISPR/Cas12 becomes evident through their applications. applications encompass medical safety, monitoring. transition conventional ultimately represents a significant milestone contaminant detection. By incorporating molecular biology, nanotechnology, bioinformatics, potential reshape landscape water CRIPSR-Cas diagnostics is transformative technology paves way for safer healthier future environment life.

Language: Английский

Citations

Challenges and perspectives of CRISPR-based technology for diagnostic applications DOI

Stefano Del Giovane,

Neda Bagheri, Andrea C. Di Pede

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 172, P. 117594 - 117594

Published: Feb. 8, 2024

The precision and versatility of CRISPR-based techniques, combined with the advantages nucleic acid-based nanotechnology, hold great promise in transforming landscape molecular diagnostics. While significant progress has been made, current platforms primarly focus on acid detection. To expand applicability fully leverage offered by diagnostics, ongoing efforts explore strategies to develop CRISPR sensors capable detecting a diverse range analytes beyond acids. In addition, challenges still persist adaptation for point-of-care (POC) applications, involving concerns such as portability automation, well complexities associated multiplexing. Here, we provide detailed classification comprehensive discussion facilitating conversion non-nucleic target binding into CRISPR-powered outputs an emphasis their corresponding design principles. Furthermore, second part review outlines potential solutions seamlessly integrating these user-friendly rapid tests specifically tailored (POC).

Language: Английский

Citations

A ratiometric fluorescent biosensing platform for ultrasensitive detection of Salmonella typhimurium via CRISPR/Cas12a and silver nanoclusters DOI

Long Ma, Junling Wang, Yaru Li

et al.

Journal of Hazardous Materials, Journal Year: 2022, Volume and Issue: 443, P. 130234 - 130234

Published: Oct. 27, 2022

Language: Английский

Citations

Comparison of CRISPR/Cas and Argonaute for nucleic acid tests DOI

Yaru Li, Dan Liao,

Jun Kou

et al.

Trends in biotechnology, Journal Year: 2022, Volume and Issue: 41(5), P. 595 - 599

Published: Dec. 7, 2022

Language: Английский

Citations