A digital microfluidic device integrated with electrochemical sensor and 3D matrix for detecting soluble PD-L1

Biosensors and Bioelectronics X, Journal Year: 2024, Volume and Issue: 19, P. 100490 - 100490

Published: May 17, 2024

PD1/PD-L1 checkpoint inhibitors are at the forefront of cancer immunotherapies. However, overall response rate remains only 10-30%. Even among initial responders, drug resistance often occurs, which can lead to prolonged use a futile therapy in race with fatal disease. It would be ideal closely monitor key indicators patients' immune responsiveness, such as circulating PD-L1 levels. Traditional detection methods, ELISA, limited sensitivity and rely on core lab facilities, preventing their for regular monitoring. Electrochemical sensors exist an attractive candidate point-of-care tool, yet, streamlining multiple processes single platform challenge. To overcome this challenge, work integrated electrochemical sensor arrays into digital microfluidic device combine distinct merits, so that soluble (sPD-L1) molecules rapidly detected programmed automated manner. This new featured microscale modified electrically conductive 3D matrix, detect low 1 pg/mL sPD-L1 high specificity. The also have desired repeatability obtain reproducible results different days. demonstrate functionality process more complex biofluids, we used secreted by human breast cell line culture media directly observed 2X increase signal compared control experiment. novel holds promise close monitoring level physiological fluids evaluate efficacy PD-1/PD-L1 immunotherapy.

Language: Английский

---

A post-pandemic perspective: Evolution of SARS-CoV-2 early detection

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 170, P. 117458 - 117458

Published: Nov. 24, 2023

Language: Английский

---

Clinical application of SARS-CoV-2 antibody detection and monoclonal antibody therapies against COVID-19

World Journal of Clinical Cases, Journal Year: 2023, Volume and Issue: 11(10), P. 2168 - 2180

Published: March 30, 2023

The purpose of this study was to investigate the clinical application severe acute respiratory distress syndrome coronavirus-2 (SARS-CoV-2) specific antibody detection and anti-SARS-CoV-2 monoclonal antibodies (mAbs) in treatment coronavirus infectious disease 2019 (COVID-19). dynamic changes SARS-CoV-2 during COVID-19 were studied. Immunoglobulin M (IgM) appeared earlier lasted for a short time, while immunoglobulin G (IgG) later longer. IgM tests can be used early diagnosis COVID-19, IgG late identification asymptomatic infected persons. combination testing nucleic acid testing, which complement each other, improve rate COVID-19. Monoclonal treat hospitalized critically ill patients non-hospitalized mild moderate patients. convalescent plasma, highly concentrated immunoglobulin, mAbs are examples products. Due continuous emergence mutated strains novel coronavirus, especially omicron, its immune escape ability infectivity enhanced, making effects authorized products reduced or invalid. Therefore, optimal (especially mAbs) is more effective conducive patient recovery.

Language: Английский

---

Combining hybrid nanoflowers with hybridization chain reaction for highly sensitive detection of SARS-CoV-2 nucleocapsid protein

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1279, P. 341838 - 341838

Published: Sept. 21, 2023

Language: Английский

---

A biosensor based on magnetoelastic waves for detection of antibodies in human plasma for COVID-19 serodiagnosis

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 261, P. 116456 - 116456

Published: June 5, 2024

Language: Английский

---

Carbon Nanostructured Immunosensing of Anti-SARS-CoV-2 S-Protein Antibodies

Molecules, Journal Year: 2023, Volume and Issue: 28(24), P. 8022 - 8022

Published: Dec. 9, 2023

The rampant spread and death rate of the recent coronavirus pandemic related to SARS-CoV-2 respiratory virus have underscored critical need for affordable, portable diagnostics, particularly in resource-limited settings. Moreover, efficient timely monitoring vaccine efficacy is needed prevent future widespread infections. Electrochemical immunosensing poses an effective alternative conventional molecular spectroscopic approaches, offering rapid, cost-effective, sensitive, electroanalysis disease biomarkers antibodies; however, efforts improve binding efficiency sensitivity are still being investigated. Graphene quantum dots (GQDs) particular shown promise improving device sensitivity. This study reports development a GQD-functionalized point-of-contamination leveraging selective interactions between SARS-CoV-2-specific Spike (S) Protein receptor domain (RBD) antigens IgG anti-SARS-CoV-2-specific S-protein antibodies at screen-printed carbon electrode (SPCE) surfaces. immunocomplexes formed GQD surfaces result interruption redox reactions that take place presence probe, decreasing current response. Increased active surface area, conductivity, via EDC/NHS chemistry were achieved due nanomaterial inclusion, with 5 nm, blue luminescent GQDs best results. concentration, ratio, RBD incubation time concentration optimized biosensor, inter- intra-screen-printed detection was investigated by calibration studies on multiple single electrodes. used entire provided label-free immunosensor able selectively detect anti-SARS-CoV-2 0.5 100 ng/mL IgM other excellent regression 0.9599. A LOD 2.028 found, comparable findings literature-reported values. sensor further compared non-specific antibodies. developed low-oxygen content nanomaterials, namely (i) dot (CQD), (ii) electrochemically reduced graphene oxide, (iii) black-functionalized devices. suggest improved electron transfer kinetics increased area CNs, along oxygen content, aid novel suggests possible application toward antibody production SARS-CoV-2-vaccinated patients immune responses, efficacy, lifetime meet demands POC analysis

Language: Английский

---

A Simplistic Label‐Free Electrochemical Immunosensing Approach for Rapid and Sensitive Detection of Anti‐SARS‐COV‐2 Nucleocapsid Antibodies

ChemistrySelect, Journal Year: 2024, Volume and Issue: 9(20)

Published: May 22, 2024

Abstract Rapid and precise detection of SARS‐CoV‐2 antibodies is paramount for effective outbreak monitoring vaccine efficacy assessment. While existing approaches antibody often rely on complex electrochemical immunosensing with nanomaterial functionalization targeting S‐protein antibodies, their limitations in sensitivity complexity have hindered widespread application. Here, we present a simplistic platform designed the rapid, specific IgG Nucleocapsid antibodies. Notably, this study marks only second exploration N‐protein detection. The utilizes traditional self‐assembled monolayers to establish selective bio‐affinity between gold electrode functionalized antigen. Interestingly, despite absence functionalization, developed achieves comparable sensors across wide range (0.025 1 ng/mL) an impressive limit (0.019 ng/mL). simplicity approach, relying solely immunocomplex reactions, underscores that binding efficiency may be achieved determines its affordability, specificity, high sensitivity. By eliminating need additional steps, offers streamlined solution demonstrates possibility as promising avenue application assessment particularly underdeveloped regions.

Language: Английский

---

Chemiresistors

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 295 - 319

Published: Nov. 1, 2024

---