Gradient Hydrogels Spatially Trapped Optical Cell Profiling for Quantitative Blood Cellular Osmotic Analysis DOI
Yantong Liu, Le Yu, Longfei Chen

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(3), P. 1592 - 1601

Published: March 13, 2024

The quantitative exploration of cellular osmotic responses and a thorough analysis pressure-responsive behaviors are poised to offer novel clinical insights into current research. This underscores paradigm shift in the long-standing approach colorimetric measurements triggered by red cell lysis. In this study, we engineered purpose-driven optofluidic platform facilitate goal. Specifically, creating photocurable hydrogel traps surmounts persistent challenge─optical signal interference from fluid disturbances. achievement ensures stable spatial phase cells acquisition optical signals for accurate response at single-cell level. Leveraging multigradient microfluidic system, constructed gradient developed an imaging recognition algorithm, empowering comprehensive behaviors. Notably, system has successfully precisely analyzed individual clustered within dimension. Prospective testing further substantiated its feasibility performance that it demonstrates accuracy 92% discriminating complete hemolysis values (n = 25) 100% identifying initial 25). Foreseeably, strategy should promise advance pressure-related analysis, benefiting investigation diagnosis related blood diseases, quality, drug development, etc.

Language: Английский

Portable and Flexible Hydrogel Sensor for On-Site Atrazine Assay on Agricultural Products DOI
Yuying Wang, Yue Wang, Yang Xue

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(19), P. 7772 - 7779

Published: May 3, 2024

There is growing attention focused toward the problems of ecological sustainability and food safety raised from abuse herbicides, which underscores need for development a portable reliable sensor simple, rapid, user-friendly on-site analysis herbicide residues. Herein, novel multifunctional hydrogel composite explored to serve as flexible facile efficient atrazine (ATZ) The electrode fabricated by doping graphite-phase carbon nitride (g-C3N4) into aramid nanofiber reinforced poly(vinyl alcohol) via simple solution-casting procedure. Benefiting excellent electroactivity large specific surface area solid nanoscale component, prepared capable sensitive detection ATZ with limit down 0.002 ng/mL per test time less than 1 min. After combination smartphone-controlled electrochemical analyzer, exhibited satisfactory analytical performance assay. We further demonstrated applications in evaluation residues real water soil samples well point-of-need on various agricultural products. envision that this will open new avenue next-generation tools monitoring environment

Language: Английский

Citations

26
Algal Biosensors for Detection of Potentially Toxic Pollutants and Validation by Advanced Methods: A Brief Review DOI Creative Commons
Diego Serrasol do Amaral, Luana Vaz Tholozan, Daisa Hakbart Bonemann

et al.

Chemosensors, Journal Year: 2024, Volume and Issue: 12(11), P. 235 - 235

Published: Nov. 13, 2024

The presence of potentially toxic pollutants, such as pesticides and metal ions, even at low concentrations, can significantly impact aquatic environmental health. This pollution is a globally widespread problem requires fast reliable analysis, especially for in-situ identification/quantification. Atomic absorption spectrometry plasma-based techniques have been considered the most analytical tools used to monitor ions in media other related matrices. dynamics global climate change its correlation with pollution, from anthropogenic sources, encouraged development faster monitoring these pollutants. A noteworthy alternative determining pollutants using algae-based biosensors, resulting cost reduction simplification enabling more comprehension role humans change. These which may not highest sensitivity quantification, demonstrated remarkable potential identification several field applications. Biosensors be an excellent biotechnology solution changes. Thus, this review highlights main advances developing comparing biosensors possibilities their possible applications analysis.

Language: Английский

Citations

3
Probe-free and wash-free strategy for on-site testing of chloramphenicol in food based on differential encodable microspheres separation system DOI

Chen Yang,

Guoqing He,

Shukun Zhao

et al.

Talanta, Journal Year: 2025, Volume and Issue: 295, P. 128250 - 128250

Published: May 4, 2025

Language: Английский

Citations

0
Gradient Hydrogels Spatially Trapped Optical Cell Profiling for Quantitative Blood Cellular Osmotic Analysis DOI
Yantong Liu, Le Yu, Longfei Chen

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(3), P. 1592 - 1601

Published: March 13, 2024

The quantitative exploration of cellular osmotic responses and a thorough analysis pressure-responsive behaviors are poised to offer novel clinical insights into current research. This underscores paradigm shift in the long-standing approach colorimetric measurements triggered by red cell lysis. In this study, we engineered purpose-driven optofluidic platform facilitate goal. Specifically, creating photocurable hydrogel traps surmounts persistent challenge─optical signal interference from fluid disturbances. achievement ensures stable spatial phase cells acquisition optical signals for accurate response at single-cell level. Leveraging multigradient microfluidic system, constructed gradient developed an imaging recognition algorithm, empowering comprehensive behaviors. Notably, system has successfully precisely analyzed individual clustered within dimension. Prospective testing further substantiated its feasibility performance that it demonstrates accuracy 92% discriminating complete hemolysis values (n = 25) 100% identifying initial 25). Foreseeably, strategy should promise advance pressure-related analysis, benefiting investigation diagnosis related blood diseases, quality, drug development, etc.

Language: Английский

Citations

2