CRISPR/Cas‐SERS Sensing Platforms: A Frontier Technology for Next‐Generation Fast, Low‐Cost, Ultra‐Micro Biosample Detection DOI
Chengxin Bao, Xiangguo Liu, Chongyang Liang

et al.

Journal of Raman Spectroscopy, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

ABSTRACT Researchers have long been interested in nucleic acid detection technology. Surface‐enhanced Raman spectroscopy (SERS) is distinguished by its high sensitivity, minimal sample volume, resistance to fluorescence interference, cost‐effectiveness, and rapidity compared conventional analysis methods. The clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein (CRISPR/Cas), a novel gene editing tool, has garnered significant interest due precise identification isothermal advantages. Integrating the CRISPR/Cas system's specific capabilities with high‐sensitivity fingerprinting properties of SERS offers sensitive, ultra‐low rapid, straightforward method for detecting new modalities. This review delineates components characteristics system, encompassing three Cas proteins (Cas9, Cas12, Cas13) technologies derived from CRISPR/Cas, namely, enzymatic reporter unlocking (SHERLOCK) DNA endonuclease‐targeted CRISPR trans reporters (DETECTR). Advancements CRISPR/Cas‐SERS‐based assays were emphasized, traditional non‐nucleic tests. Examples encompass microfluidics/microdroplet‐based CRISPR/Cas‐SERS non‐amplified based on CRISPR/Cas‐SERS, so on.

Language: Английский

Advances in Functional Nucleic Acid SERS Sensing Strategies DOI
Lin Shi, Yukang Liu, Xiaodong Li

et al.

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Functional nucleic acids constitute a distinct category of that diverge from conventional acid amplification methodologies. They are capable forming intricate hybrid structures through Hoogsteen and reverse hydrogen bonding interactions between double-stranded single-stranded DNA, thereby broadening the spectrum DNA interactions. In recent years, functional DNA/RNA-based surface-enhanced Raman spectroscopy (SERS) has emerged as potent platform ultrasensitive multiplexed detection variety analytes interest. This review aims to elucidate operational principles several in SERS detection, including DNAzymes, G-quadruplexes, aptamers, CRISPR, origami etc., alongside design methodologies practical applications sensing. Initially, an overview is summarized encompassing structural attributes sensing mechanisms inherent diverse DNA/RNA. Following this, various innovative strategies for constructing acid-based sensors illustrated detail, aimed at improving present capabilities. A comprehensive summing up then conducted on these crucial fields, such disease diagnosis, environmental monitoring, food safety with particular focus sensitivity, specificity, analytical versatility. Finally, conclusive remarks offered along exploration existing challenges prospective avenues future research this developed field.

Language: Английский

Citations

6
SATCAS: A CRISPR/Cas13a-based simultaneous amplification and testing platform for one-pot RNA detection and SNPs distinguish in clinical diagnosis DOI Creative Commons
Ting Wang,

Linlin Bai,

Guoling Wang

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 263, P. 116636 - 116636

Published: Aug. 5, 2024

The clinical diagnosis of pathogen infectious diseases increasingly requires sensitive and rapid RNA detection technologies. RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a system has shown immense potential in molecular diagnostics due to its trans-cleavage activity. However, most Cas13a-based methods require an amplicon transcription step, the multi-step open-tube operations are prone contamination, limiting their widespread application. Here, we propose ultrasensitive (single-copy range, ∼aM) (within 40 min) isothermal one-pot platform, termed SATCAS (Simultaneous Amplification Testing platform based on Cas13a). This method effectively distinguishes viable bacteria (0%-100%) under constant total bacterial conditions, demonstrating robustness universality. excels identifying single nucleotide polymorphisms (SNPs), particularly detecting 0.5% drug-resistant mutations. We validated by infections biological samples from 68 HBV, 23 EBV, 48 SARS-CoV-2 patients, achieving 100% sensitivity, 92.86% specificity, 97.06% accuracy HBV infection testing. anticipate that broad application early diagnosis, subtyping, drug resistance detection, point-of-care monitoring diseases.

Language: Английский

Citations

12
A CRISPR/Cas13a system based on a dumbbell-shaped hairpin combined with DNA-PAINT to establish the DCP-platform for highly sensitive detection of Hantaan virus RNA DOI
Jiayong Zhong, Ziyue Xu, Jiawei Peng

et al.

Talanta, Journal Year: 2025, Volume and Issue: 291, P. 127852 - 127852

Published: March 6, 2025

Language: Английский

Citations

1
Dual-sensitized heterojunction Ag2S/ZnS/NiS composites with entire visible-light region absorption for ultrasensitive photoelectrochemical detection of tobramycin DOI

Yushuang Jin,

Wanqing Yu, Qiang Chen

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 260, P. 116459 - 116459

Published: June 1, 2024

Language: Английский

Citations

3
A Crispr/Cas13a-Dumbbell Hairpin Dna-Paint Platform for Ultrasensitive Detection of Hantaan Virus Rna DOI
Jiayong Zhong, Ziyue Xu, Jiawei Peng

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
CRISPR/Cas-Based Nanobiosensor Using Plasmonic Nanomaterials to Detect Disease Biomarkers DOI Creative Commons
Jin‐Ha Choi, Jinho Yoon,

Meizi Chen

et al.

BioChip Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Language: Английский

Citations

0
Catalytic hairpin assembly assists CRISPR/Cas12a-mediated high-sensitivity detection of aflatoxin B1 DOI
Shuang Liu,

Ji Hu,

Ruifeng Zhang

et al.

Talanta, Journal Year: 2025, Volume and Issue: 293, P. 128043 - 128043

Published: April 8, 2025

Language: Английский

Citations

0
Multivalent aptamer-linked tetrahedron DNA assisted catalytic hairpin assembly for accurate SERS assay of cancer-derived exosomes in clinical blood DOI
Jingjing Zhang,

Chenlong Yan,

Lijie Xie

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 282, P. 117497 - 117497

Published: April 18, 2025

Language: Английский

Citations

0
CRISPR/Cas‐SERS Sensing Platforms: A Frontier Technology for Next‐Generation Fast, Low‐Cost, Ultra‐Micro Biosample Detection DOI
Chengxin Bao, Xiangguo Liu, Chongyang Liang

et al.

Journal of Raman Spectroscopy, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

ABSTRACT Researchers have long been interested in nucleic acid detection technology. Surface‐enhanced Raman spectroscopy (SERS) is distinguished by its high sensitivity, minimal sample volume, resistance to fluorescence interference, cost‐effectiveness, and rapidity compared conventional analysis methods. The clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein (CRISPR/Cas), a novel gene editing tool, has garnered significant interest due precise identification isothermal advantages. Integrating the CRISPR/Cas system's specific capabilities with high‐sensitivity fingerprinting properties of SERS offers sensitive, ultra‐low rapid, straightforward method for detecting new modalities. This review delineates components characteristics system, encompassing three Cas proteins (Cas9, Cas12, Cas13) technologies derived from CRISPR/Cas, namely, enzymatic reporter unlocking (SHERLOCK) DNA endonuclease‐targeted CRISPR trans reporters (DETECTR). Advancements CRISPR/Cas‐SERS‐based assays were emphasized, traditional non‐nucleic tests. Examples encompass microfluidics/microdroplet‐based CRISPR/Cas‐SERS non‐amplified based on CRISPR/Cas‐SERS, so on.

Language: Английский

Citations

0