Three-Way Junction Probe triggered CRISPR/Cas14a1 enhanced EXPonential Amplification Reaction for Sensitive Pseudomonas aeruginosa Detection DOI
Yu Chen, Yang Liu, Wen Zhang

et al.

Analytical Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 13, 2024

Pseudomonas aeruginosa ( P. , PA) is a rod-shaped Gram-negative opportunistic bacterium capable of causing nosocomial infections, such as burn wound infections and meningitis.

Language: Английский

Amplification-Free Detection of Mycobacterium Tuberculosis Using CRISPR-Cas12a and Graphene Field-Effect Transistors DOI
Weiqi Wang, Hongqiang Du, Changhao Dai

et al.

Nanoscale, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Current molecular tests for tuberculosis (TB), such as whole genome sequencing and Xpert Mycobacterium tuberculosis/rifampicin resistance assay, exhibit limited sensitivity necessitate the pre-amplification step of target DNA. This limitation greatly increases detection time poses an increased risk infection. Here, we present a graphene field-effect transistor (GFET) based on CRISPR/Cas system detecting tuberculosis. The CRISPR/Cas12a has ability to specifically recognize cleave By integrating onto FET platform utilizing its electrical amplification capability, achieve rapid sensitive without requiring sample pre-amplification, with limit (LoD) low 2.42 × 10-18 M. Cas12a-GFET devices can differentiate 30 positive cases from 56 serum samples within 5 minutes. These findings highlight immense potential in future biological analysis clinical diagnosis.

Language: Английский

Citations

1
One‐Step RAA and CRISPR‐Cas13a Method for Detecting Influenza B Virus DOI Creative Commons
Xinling Zhang, Shiyu Chen,

Juezhuo Li

et al.

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(4)

Published: April 1, 2025

ABSTRACT We developed a sensitive and specific method based on recombinase‐aided amplification (RAA) clustered regularly interspaced short palindromic repeats (CRISPR)‐CRISPR‐associated protein 13a (Cas13a). This method, named CRISPR‐based Rapid Efficient Test (CRISPRET), is designed for the early diagnosis of Influenza B (FluB) with aim shortening its transmission chain. identified conserved regions in Virus (IBV) NS gene forward reverse primers along crRNAs. then established optimised reaction system, Nucleic Acid Positive Reference Materials IBV were used to evaluate detection limit (DL) CRISPRET. Additionally, we collected 257 clinical samples, comprising 127 samples from patients infection 130 healthy individuals, subjected them dual using CRISPRET qPCR positive predictive value (PPV), negative (NPV), sensitivity specificity one primer, two primers, crRNAs establish optimise CRISPR ET. The demonstrated DL 500 copies·μL −1 when assisted by appropriate equipment. Despite requiring auxiliary equipment 30‐min reaction, ET enables nucleic acid within approximately first 5 min, achieving high (100%), (97.69%), PPV (97.69%) NPV concordance rate 98.83% qPCR. offers simple, field‐applicable, one‐step rapid IBV. It has strong potential field‐testing applications intelligent integration into existing diagnostic systems.

Language: Английский

Citations

0
Facilitating self-testing with a fast, accurate, and simplified shelf-stable colorimetric LAMP system for Mpox and SARS-CoV-2 detection DOI
Nelson Odiwuor, Junhua Li,

Ping He

et al.

Talanta, Journal Year: 2024, Volume and Issue: 283, P. 127119 - 127119

Published: Nov. 4, 2024

Language: Английский

Citations

1
Three-Way Junction Probe triggered CRISPR/Cas14a1 enhanced EXPonential Amplification Reaction for Sensitive Pseudomonas aeruginosa Detection DOI
Yu Chen, Yang Liu, Wen Zhang

et al.

Analytical Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 13, 2024

Pseudomonas aeruginosa ( P. , PA) is a rod-shaped Gram-negative opportunistic bacterium capable of causing nosocomial infections, such as burn wound infections and meningitis.

Language: Английский

Citations

1